Inhibitors of cathepsin S

ABSTRACT

The present invention provides compounds, compositions and methods for the selective inhibition of cathepsin S. In a preferred aspect, cathepsin S is selectively inhibited in the presence of at least one other cathepsin isozyme. The present invention also provides methods for treating a disease state in a subject by selectively inhibiting cathepsin S.

CROSS-REFERENCE TO RELATED APPLICATIONS

This present application claims priority to U.S. Patent Application No. 60/496,980, filed Aug. 20, 2003, the teaching of which is hereby incorporated by reference in its entirety for all purposes.

BACKGROUND OF THE INVENTION

Cysteine proteases represent an enzymatic class of proteins that catalyze the hydrolysis of peptide bonds by a nucleophilic sulfhydryl group of a cysteine residue in the active site of the enzyme. Several normal and disease processes in mammals have been associated with cysteine protease activity and include, but are not limited to: osteoporosis, osteoarthritis (Inui, T., O. Ishibashi, J Biol Chem 1997, 272(13), 8109-12; Saftig, P., E. Hunziker, et al., Adv Exp Med Biol 2000+ADs 2000, 477, 293-303; Saftig, P., E. Hunziker, et al., Proc Natl Acad Sci USA 1998, 95(23), 13453-8), periodontal diseases, Paget's disease, atherosclerosis (Jormsjo, S., D. M. Wuttge, et al., Am J Pathol 2002 161(3), 939-45), multiple sclerosis (Beck, H., G. Schwarz, et al., Eur J Immunol 2001, 31(12), 3726-36), rheumatoid arthritis (Nakagawa, T. Y., W. H. Brissette, et al., Immunity 1999, 10(2), 207-17; Hou, W. S., Z. Li, et al., Am J Pathol 2001, 159(6), 2167-77), juvenile onset diabetes, lupus, asthma (Cimerman, N., P. M. Brguljan, et al., Pflugers Arch 2001, 442(6 Suppl 1), R204-6), tissue rejection, Alzheimer's disease (Lemere, C. A., J. S. Munger, et al., Am J Pathol 1995, 146(4), 848-60), Parkinson's disease (Liu, Y., L. Fallon, et al., Cell 2002, 111(2), 209-18), neuronal degeneration, shock (Jaeschke, H., M. A. Fisher, et al., J Immunol 1998, 160(7), 3480-6), cancer (Fernandez, P. L., X. Farre, et al., Int J Cancer 2001, 95(1), 51-5), malaria (Malhotra, P., P. V. Dasaradhi, et al., Mol Microbiol 2002, 45(5), 1245-54), Chagas (Eakin, A. E., A. A. Mills, et al., J Biol Chem 1992, 267(11), 7411-20), leishmaniasis, shistosomiasis, and African trypanosomiasis (Caffrey, C. R., S. Scory, et al., Curr Drug Targets 2000, 1(2), 155-62; Lalmanach, G., A. Boulange, et al., Biol Chem 2002, 383(5), 739-49).

Cathepsins are a subclass of cysteine protease that belong to the enzyme classification EC 3.4.22 (Barrett, A. J., N. D. Rawlings, et al., Handbook of proteolytic enzymes. London, Academic Press). Cathepsins play a major role in lysosomal, endosomal, and extracellular protein degradation and have thus been implicated in many disease processes. For example, Cathepsin B [EC 3.4.22.1] has been postulated to play a role in tumor metastasis (Berquin, I. M. and B. F. Sloane Adv Exp Med Biol 1996, 389, 281-94).

Cathepsin S [EC 3.4.22.27] is largely expressed in professional antigen presenting cells such as macrophages and dendritic cells. Cathepsin S has been shown to be required for proper MHC class II antigen presentation (Shi, G. P., J. A. Villadangos, et al., Immunity 1999, 10(2) 197-206). As a result of its non-redundant role in MHC class II antigen presentation, cathepsin S has been associated with inflammation, arthritis, and atherosclerosis. The selective expression of cathepsin K [EC 3.4.22.38] in osteoclasts coupled with the ability of cathepsin K to degrade type I collagen suggests that it plays a role in normal and pathogenic bone remodeling (Bromme, D., K. Okamoto, et al., J Biol Chem 1996, 271(4), 2126-32). There is a need in the art for compounds and methods that selectively inhibit specific cysteine proteases for treating several pathogenic disorders in mammals. The present invention satisfies these and other needs.

SUMMARY OF THE INVENTION

The present invention provides compounds, compositions and methods for the selective inhibition of cathepsin S. The compounds of the present invention are selective for cathepsin S in the presence of other cathepsin isozymes. In a preferred embodiment, the compounds of the present invention are selective for cathepsin S in the presence of cathepsin K, L, B, or combinations thereof. The present invention also provides methods for treating a disease state in a subject by selectively inhibiting cathepsin S in the presence of other cathepsin isozymes. In a preferred aspect, cathepsin S is selectively inhibited in the presence of cathepsin K, L, B, or combinations thereof.

In one aspect, the present invention provides compounds of Formula I:

-   -   or a pharmaceutically acceptable salt or prodrug thereof,         wherein:     -   Q is a heterocycle selected from the group consisting of         azetidinyl, pyrrolidinyl, piperidyl, morpholinyl,         thiomorpholinyl, piperazinyl and indolinyl substituted with 0-2         R^(Q), wherein Q is connected to —C(═O)— via a ring nitrogen         atom; and NR²⁵R²⁶;     -   each R^(Q) is independently a member selected from the group         consisting of OH, F, Cl, —S(═O)₂CH₃—, acetyl, ═O, C₁-C₆ alkyl,         C₁-C₆ alkoxy, CF₃, OCF₃ and NR¹⁰R¹¹;     -   A is a member selected from the group consisting of —O—CR¹R²—,         —NH—CR¹R²—, —CR³R⁴—O—, and —CR³R⁴—CR¹R²—;     -   each of R¹ and R³ is independently a member selected from the         group consisting of H, a C₁-C₆ alkoxy, a C₁-C₆ alkyl substituted         with 0-2 R^(1a), wherein said C₁-C₆ alkyl may optionally contain         a heteroatom selected from the group consisting of —O—, —S—,         —S(═O)— and —S(═O)₂—; a C₂-C₆ alkenyl, a C₃-C₆ alkynyl, a C₃-C₇         cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl         substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³, a         5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-3 R¹³;     -   each R^(1a) is independently a member selected from the group         consisting of a C₆-C₁₀ aryl substituted with 0-3 R¹³, a 5- to         6-membered monocyclic or 8- to 10-membered bicyclic heteroaryl         containing 1 to 4 heteroatoms each independently a member         selected from the group consisting of N, O and S, wherein said         heteroaryl is substituted with 0-3 R¹³, a C₃-C₈ cycloalkyl         substituted with 0-2 R^(Q), a C₇-C₁₁ bicycloalkyl substituted         with 0-2 R^(Q), and a C₁-C₃ perfluoroalkyl;     -   each of R² and R⁴ is independently a member selected from the         group consisting of H, F, OH, C₁-C₆ alkyl and C₁-C₆ alkoxy;     -   R⁵ is a member selected from the group consisting of H,         C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, phenyl substituted with 0-2 R¹³, and a         5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-2 R¹³,         C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-2 R²¹, wherein         said C₁-C₆ alkyl may optionally contain a heteroatom selected         from the group consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and         —NR²²—;     -   each of R⁶, R⁷, R⁸ and R⁹ is independently a member selected         from the group consisting of H and C₁-C₆ alkyl;     -   alternatively, R⁵ and R⁷ are taken together to form a C₅-C₇         cycloalkyl, wherein a methylene of said C₅-C₇ cycloalkyl may         optionally be replaced with a heteroatom selected from the group         of —O—, —S—, —S(═O)—, and —S(═O)₂—;     -   each R¹⁰ is independently a member selected from the group         consisting of H, C₁-C₄ alkyl, (C₁-C₄ alkyl)-C(═O)— and (C₁-C₄         alkyl)-S(═O)₂—;     -   each R¹¹ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl;     -   each R¹² is independently a member selected from the group         consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3         R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms         each independently a member selected from the group consisting         of N, O and S, wherein said 5- to 6-membered heteroaryl is         substituted with 0-3 R¹³, and a C₁-C₆ alkyl substituted with 0-1         R¹⁹;     -   each R¹³ is independently a member selected from the group         consisting of H, OH, F, Cl, Br, CN, NO₂, COOR¹⁷, C(═O)NR¹⁷R¹⁸,         S(═O)₂NR¹⁷R¹⁸, acetyl, —SCH₃, —S(═O)CH₃, —S(═O)₂CH₃, —NR¹⁰R¹¹,         C₁-C₆ alkoxy, C₁-C₃ perfluoroalkyl, C₁-C₃ perfluoroalkoxy and a         C₁-C₆ alkyl;     -   each R¹⁴ is independently a member selected from the group         consisting of H, C₃-C₇ cycloalkyl, C₁-C₄ alkyl substituted with         0-1 R¹⁹, and a phenyl substituted with 0-3 R¹³;     -   each R¹⁵ is independently a member selected from the group         consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3         R¹³, and a C₁-C₆ alkyl substituted with 0-1 R¹⁹;     -   each R¹⁶ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl;     -   alternatively, R¹⁵ and R¹⁶ on the same N atom are taken together         to form a C₅-C₇ heterocycle containing 1-2 heteroatoms each         independently a member selected from the group consisting of N,         O and S;     -   each of R¹⁷ and R¹⁸ is independently a member selected from the         group consisting of H, C₁-C₄ alkyl and C₃-C₆ cycloalkyl;     -   each R¹⁹ is independently a member selected from the group         consisting of H, C₃-C₇ cycloalkyl, a phenyl substituted with 0-3         R¹³ and a 5- to 6-membered heteroaryl containing 1 to 4         heteroatoms each independently a member selected from the group         consisting of N, O and S, wherein said 5- to 6-membered         heteroaryl is substituted with 0-3 R¹³;     -   Ar is a member selected from the group consisting of phenyl         substituted with 0-3 R²⁰, and a 5- to 10-membered heteroaryl         containing 1 to 4 heteroatoms each independently a member         selected from the group consisting of N, O and S; wherein said         heteroaryl is substituted with 0-3 R²⁰;     -   each R²⁰ is independently a member selected from the group         consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃,         S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, —S(═O)₂NH(C═O)CH₃, C(═O)NR¹⁷R¹⁸,         CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃ and OCF₂H;     -   alternatively, R²⁰ and R⁹ are taken together to form a 5- to         7-membered heterocyclic ring containing 1-2 heteroatoms each         independently a member selected from the group consisting of N,         O and S; wherein said 5 to 7 membered heterocyclic ring is         ortho-fused to Ar; wherein said 5- to 7-membered heterocyclic         ring may be optionally substituted with 0-2 R²⁴;     -   each R²¹ is independently a member selected from the group         consisting of H, OH, F, Cl, CN, NO₂, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶,         NR²²R²³, C₁-C₃ perfluoroalkoxy, C₁-C₄ alkoxy, C₂-C₄ alkenyl,         C₂-C₄ alkynyl, phenyl substituted with 0-3 R¹³, a 5- to         6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-3 R¹³,         C₃-C₈ heterocycle containing 1 to 2 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heterocycle is substituted with 0-2 R¹³         and is saturated or partially unsaturated, and C₃-C₈ cycloalkyl;

-   R²² is independently a member selected from the group consisting of     H, ^(t)BOC, Cbz, C₃-C₈ cycloalkyl, (C₁-C₆ alkyl)-C(═O)—, (C₁-C₆     alkyl)-S(═O)₂—, a C₁-C₆ alkyl substituted with 0-1 R¹⁹, a phenyl     substituted with 0-3 R¹³ and a 5- to 6-membered heteroaryl     containing 1 to 4 heteroatoms each independently a member selected     from the group consisting of N, O and S, wherein said 5- to     6-membered heteroaryl is substituted with 0-3 R¹³;     -   each R²³ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl.     -   each R²⁴ is independently a member selected from the group         consisting of C₁-C₄ alkyl, F, Cl and C₁-C₄ alkoxy, CF₃ and OCF₃;     -   alternatively, two R²⁴ may be combined to form C₃-C₆ cycloalkyl.     -   each of R²⁵ and R²⁶ is independently a member selected from the         group consisting of C₁-C₆ alkyl, wherein said C₁-C₆ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and —NR₂₂—.

In a second aspect, the present invention provides a pharmaceutical composition comprising a compound of Formula I, as described above, and a pharmaceutically acceptable excipient.

In a third aspect, the present invention provides a method of selectively inhibiting the cathepsin S activity in a mammal in need thereof, comprising administering to the mammal a therapeutically effective amount of a compound of Formula I, as described above, or a pharmaceutically acceptable salt or prodrug thereof.

These and other aspects, objects and embodiments will become more apparent when read with the accompanying figure and detailed description which follows.

BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 depicts MHC II antigen presentation.

DETAILED DESCRIPTION OF THE INVENTION

I. Definitions

Unless defined otherwise, all technical and scientific terms used herein generally have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Generally, the nomenclature used herein and the laboratory procedures for organic and analytical chemistry are those well known and commonly employed in the art.

As used in this disclosure, the following abbreviations and terms have the defined meaning, unless expressly modified in the context in which the term is used: Ac acetyl Bn benzyl Boc t-butoxycarbonyl Cbz or Z benzyloxycarbonyl DCC N,N′-dicyclohexylcarbodiimide DCM dichoromethane DIBAL diisobutylaluminum hydride DIC N,N′-diisopropylcarbodiimide DIEA or DIPEA diisopropylethylamine DMAP 4-(dimethylamino)pyridine DMF dimethylformamide DMSO dimethyl sulfoxide EDC or EDCI 1-ethyl-3-(dimethylaminopropyl)-carbodiimide Fmoc 9-fluorenylmethoxycarbonyl HATU O-(7-azabenzoatriazol-1-yl)-N,N,N′,N′- tetramethyluronium hexafluorophosphate HOBt 1-hydroxybenzotriazole KHMDS potassium hexamethyldisilazide LAH lithium aluminum hydride LDA lithium diisopropylamide LHMDS lithium hexamethyldisilazide m-CPBA m-chloroperbenzoic acid MW microwave NaHMDS sodium hexamethyldisilazide PG protecting group PTSA p-toluenesulfonic acid Py pyridine RT or rt room temperature TEA triethylamine Tf trifluoromethanesulfonyl TFA trifluoroacetic acid THF tetrahydrofuran Tol p-tolyl

The term “lower” referred to above and hereinafter in connection with organic radicals or compounds respectively defines a compound or radical which can be branched or unbranched with up to and including 7, preferably up to and including 4 and (as unbranched) one or two carbon atoms.

The term “perfluoro” referred to above and hereinafter in connection with organic radicals or compounds respectively, defines a compound or radical which has at least two available hydrogens substituted with fluorine. For example, perfluorophenyl refers to 1,2,3,4,5-pentafluorophenyl, perfluoromethane refers to 1,1,1-trifluoromethyl, and perfluoromethoxy refers to 1,1,1-trifluoromethoxy.

An alkyl group is branched or unbranched and contains 1 to 7 carbon atoms, preferably 1-4 carbon atoms. Alkyl represents, for example, methyl, ethyl, propyl, butyl, isopropyl or isobutyl.

Alkenyl represents either straight chain or branched alkenyl of 2 to 7 carbon atoms, preferably 2-4 carbon atoms, e.g. as vinyl, propenyl, isopropenyl, butenyl, isobutenyl or butadienyl.

Alkynyl represents either straight chain or branched alkynyl of 2 to 7 carbon atoms, preferably 2-4 carbon atoms, e.g. as acetylenyl, propynyl, isoprpropynyl, butynyl or isobutynyl.

Alkyl, alkenyl or alkynyl can be substituted by up to 3 substituents selected from alkoxy, aryl, heterocyclyl, hydroxy, halogen, cyano, optionally substituted amino, or optionally substituted amino-oxy or trifluoromethyl.

Alkylene represents either straight chain or branched alkylene of 1 to 7 carbon atoms, i.e. a divalent hydrocarbon radical of 1 to 7 carbon atoms; for instance, straight chain alkylene being the bivalent radical of Formula —(CH₂)_(n), where n is 1, 2, 3, 4, 5, 6 or 7. Preferably alkylene represents straight chain alkylene of 1 to 4 carbon atoms, e.g. a methylene, ethylene, propylene or butylene chain, or the methylene, ethylene, propylene or butylene chain mono-substituted by C₁-C₃-alkyl (preferably methyl) or disubstituted on the same or different carbon atoms by C₁-C₃-alkyl (preferably methyl), the total number of carbon atoms being up to and including 7.

An alkoxy (or alkyloxy) group preferably contains 1-7 carbon atoms, more preferably 1-6 carbon atoms, and represents for example ethoxy, propoxy, isopropoxy, isobutoxy, preferably methoxy. Alkoxy includes cycloalkyloxy and cycloalkyl-alkyloxy.

Halogen (halo) preferably represents chloro or fluoro, but may also be bromo or iodo.

Aryl represents monocyclic, bicyclic or tricyclic aryl, for example, phenyl or phenyl mono-, di- or tri-substituted by one, two or three radicals selected from alkyl, alkoxy, aryl, hydroxy, halogen, cyano, amino, amino-alkyl, trifluoromethyl, alkylenedioxy and oxy-C₂-C₃-alkylene; all of which are optionally further substituted, for instance as hereinbefore defined; or 1- or 2-naphthyl; or 1- or 2-phenanthrenyl. Alkylenedioxy is a divalent substitute attached to two adjacent carbon atoms of phenyl, e.g. methylenedioxy or ethylenedioxy. Oxy-C₂-C₃-alkylene is also a divalent substituent attached to two adjacent carbon atoms of phenyl, e.g. oxyethylene or oxypropylene. An example for oxy-C₂-C₃-alkylene-phenyl is 2,3-dihydrobenzofuran-5-yl.

Preferred as aryl is naphthyl, phenyl or phenyl mono- or disubstituted by alkoxy, phenyl, halogen, alkyl or trifluoromethyl, especially phenyl or phenyl-mono- or disubstituted by alkoxy, halogen or trifluoromethyl, and in particular phenyl.

Examples of substituted phenyl groups as R are, e.g. 4-chlorophen-1-yl, 3,4-dichlorophen-1-yl, 4-methoxyphen-1-yl, 4-methylphen-1-yl, 4-aminomethylphen-1-yl, 4-methoxyethylaminomethylphen-1-yl, 4-hydroxyethylaminomethylphen-1-yl, 4-hydroxyethyl-(methyl)-aminomethylphen-1-yl, 3-aminomethylphen-1-yl, 4-N-acetylaminomethylphen-1-yl, 4-aminophen-1-yl, 3-aminophen-1-yl, 2-aminophen-1-yl, 4-phenyl-phen-1-yl, 4-(imidazol-1-yl)-phen-yl, 4-(imidazol-1-ylmethyl)-phen-1-yl, 4-(morpholin-1-yl)-phen-1-yl, 4-(morpholin-1-ylmethyl)-phen-1-yl, 4-(2-methoxyethylaminomethyl)-phen-1-yl and 4-(pyrrolidin-1-ylmethyl)-phen-1-yl, 4-(thiophenyl)-phen-1-yl, 4-(3-thiophenyl)-phen-1-yl, 4-(4-methylpiperazin-1-yl)-phen-1-yl, and 4-(piperidinyl)-phenyl and 4-(pyridinyl)-phenyl optionally substituted in the heterocyclic ring.

Benzyl represents a phenyl-CH₂— group. Substituted benzyl means a benzyl group in which the phenyl ring is substituted with one or more ring system substituents. Representative benzyl groups include 4-bromobenzyl, 4-methoxybenzyl, 2,4-dimethoxybenzyl, and the like.

Heteroaryl represents monocyclic or bicyclic heteroaryl, for example pyridyl, indolyl, indazolyl, quinoxalinyl, quinolinyl, isoquinolinyl, benzothienyl, benzofuranyl, benzopyranyl, benzothiopyranyl, furanyl, pyrrolyl, thiazolyl, benzothiazolyl, oxazolyl, isoxazolyl, triazolyl, tetrazolyl, pyrazolyl, imidazolyl, thienyl, or any other radicals substituted, especially mono- or di-substituted, by e.g. alkyl, nitro or halogen. Pyridyl represents 2-, 3- or 4-pyridyl, advantageously 2- or 3-pyridyl. Thienyl represents 2- or 3-thienyl. Quinolinyl represents preferably 2-, 3- or 4-quinolinyl. Isoquinolinyl represents preferably 1-, 3- or 4-isoquinolinyl. Benzopyranyl, benzothiopyranyl represents preferably 3-benzopyranyl or 3-benzothiopyranyl, respectively. Thiazolyl represents preferably 2- or 4-thiazolyl, and most preferred, 4-thiazolyl. Triazolyl is preferably 1-, 2- or 5-(1,2,4-triazolyl). Tetrazolyl is preferably 5-tetrazolyl.

Preferably, heteroaryl is pyridyl, indolyl, quinolinyl, pyrrolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, pyrazolyl, imidazolyl, thienyl, furanyl, benzothiazolyl, benzofuranyl, isoquinolinyl, benzothienyl, oxazolyl, indazolyl, or any of the radicals substituted, especially mono- or di-substituted.

Biaryl may preferably be, e.g., biphenyl, namely 2, 3 or 4-biphenyl, preferably, 4-biphenyl, each optionally substituted by, e.g., alkyl, alkoxy, halogen, trifluoromethyl or cyano, or heterocyclic-carbocyclic biaryl, preferably, e.g., thienylphenyl, pyrrolylphenyl and pyrazolylphenyl.

Cycloalkyl represents a saturated cyclic hydrocarbon optionally substituted by alkyl which contains 3 to 10 ring carbons and is advantageously cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl optionally substituted by alkyl.

Amino can be optionally substituted by, e.g., alkyl.

Carbocyclic represents a saturated or partially unsaturated cyclic hydrocarbon with 5 to 7 ring members, wherein 1 to 2 ring members can optionally be replaced with one of the following groups: —O—, —S—, —S(═O)—, —S(═O)₂— and —NR—, wherein R is a radical of the present invention.

Heterocyclyl represents a saturated cyclic hydrocarbon containing one or more, preferably 1 or 2, hetero atoms selected from O, N or S, and from 3 to 10, preferably 5 to 8, ring atoms; for example, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyrrolyl, piperidinyl, piperazinyl or morpholino; all of which can be optionally substituted, for instance as hereinbefore defined for aryl.

Pharmaceutically acceptable salts of the acidic compounds of the present invention are salts formed with bases, namely cationic salts such as alkali and alkaline earth metal salts, such as sodium, lithium, potassium, calcium, magnesium, as well as ammonium salts, such as ammonium, trimethyl-ammonium, diethylammonium, and tris-(hydroxymethyl)-methyl-ammonium salts.

Similarly acid addition salts, such as of mineral acids, organic carboxylic and organic sulfonic acids, e.g., hydrochloric acid, methanesulfonic acid, maleic acid, are also possible provided a basic group, such as pyridyl, constitutes part of the structure.

“Treat”, “treating” and “treatment” refer to a method of alleviating or abating a disease and/or its attendant symptoms.

“Inhibition”, “inhibits” and “inhibitor” refer to a compound that prohibits, or a method of prohibiting, a specific action or function.

“Inhibition constant”, K_(i), is the dissociation constant of the enzyme-inhibitor complex, or the reciprocal of the binding affinity of the inhibitor to the enzyme. For classical inhibition the value of K_(i) is much greater than the enzyme concentration and the K_(i) can be measured by monitoring the rate of reaction for a competitive substrate at multiple inhibitor concentrations. The inhibited rates are then fit by nonlinear regression to the following equation: ${v_{i}/v_{o}} = \frac{K_{m} + \lbrack S\rbrack}{{K_{m}\left( {1 + {\lbrack I\rbrack/K_{i}}} \right)} + \lbrack S\rbrack}$ where v_(o) is the initial rate of substrate processing in the absence of inhibitor, v_(i) is the initial rate of substrate processing at a concentration [I] of inhibitor, K_(m) is the steady state Michaelis constant (Fersht, A. Structure and Mechanism in Protein Science. New York, W. H. Freeman and Company, 1999), and [S] is the concentration of competitive substrate.

The assumption being made for the classical inhibition described above is that the free inhibitor concentration is equal to the total inhibitor concentration. For inhibitors that have K_(i)'s that are approximately equal to the enzyme concentration [E], the assumption that the free inhibitor concentration is equal to the total inhibitor concentration is no longer valid and an alternative equation has to be fit for determination of the apparent inhibition constant, K_(i) ^(app) using described methods (Kuzmic, P., K. C. Elrod, et al., Anal Biochem 2000, 286(1), 45-50): ${v_{i}/v_{o}} = {\frac{\lbrack E\rbrack - \lbrack I\rbrack - K_{i}^{app} + {{SQRT}\left( {\left( {\lbrack E\rbrack - {\lbrack I\rbrack K_{i}^{app}}} \right)^{2} + {{4\lbrack E\rbrack}K_{i}^{app}}} \right)}}{2\lbrack E\rbrack}.}$ The inhibition constant, K_(i), can be determined from the apparent inhibition constant, K_(i) ^(app), for competitive inhibitors by using the following relationship: $K_{i} = {\frac{K_{i}^{app}}{1 + {\lbrack S\rbrack/K_{m}}}.}$

Polycyclic ring systems in which any two adjacent rings have two (e.g., only two), adjacent atoms in common are said to be “ortho-fused”. Such ring systems have n common sides and 2n common atoms.

“Therapeutically effective amount” refers to that amount of the compound being administered sufficient to prevent development of or alleviate to some extent one or more of the symptoms of the condition or disorder being treated.

“Composition” as used herein is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. By “pharmaceutically acceptable” it is meant the carrier, diluent or excipient must be compatible with the other ingredients of the Formulation and deleterious to the recipient thereof.

“Subject” refers to animals such as mammals, including, but not limited to, primates (e.g., humans), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice and the like. In certain aspects, the subject is a human.

II. General

Cathepsin S is a cysteine protease that has been associated with several normal and disease processes in mammals. Specifically, cathepsin S has been directly associated with inflammation, arthritis, and atherosclerosis, as a result of its role in MHC class II antigen presentation. In a preferred aspect, the present invention provides compounds that inhibit the activity of cathepsin S. The present invention also provides methods for treating several disease states in mammals by inhibiting the activity of cathepsin S. In a more preferred aspect, the compounds of the present invention selectively inhibit cathepsin S in the presence of at least one cathepsin isozyme.

III. Compounds

A. Preparation of Compounds

In one embodiment, the arylaminoethylamines 1A (Scheme 1) used in the present invention can be prepared by a decarboxylative ring opening of oxazolidin-2-one with an aromatic amine as described in E. Altman et al., J. Med Chem. 2002, 45, 2352-54 and references cited therein.

Another synthetic route to the diamines used in the present invention is illustrated in Scheme 2.

A N-protected amino acid can be reduced using either a BH₃ method or NaBH₄ reduction of the corresponding mixed anhydride [see R. C. Larock A guide to functional group preparations pp. 548-552, Wiley-VCH, 1989] to obtain 2A (Scheme 2). One can then oxidize the alcohol to the aldehyde and reductively aminate the resulting aldehyde with an amine to afford 2B. This intermediate can then be deprotected using the appropriate reagents for the PG, such as TFA for Boc.

Synthetic approaches to indolines used in this invention are widely described in the literature and well know to one skilled in the art. Typical methods include, but are not limited to, the methods disclosed in the following references: (a) G. W. Gribble et al., Synthesis 1977, 859; (b) A. Smith et al., Chem. Commun. 1965, 427; (c) G. W. Gribble et al., J. Am. Chem. Soc. 1974, 96, 7812; (d) J. G. Berger Synthesis 1974, 508; (e) L. J. Dolby et al., J. Heterocycl. Chem. 1966, 3, 124; (f) W. A. Remers et al., J. Org. Chem. 1971, 36, 279; (g) S. O'Brien et al., J. Chem. Soc. 1960, 4609; (h) Y. Kikugawa et al., Synthesis 1978, 477.

Synthetic approaches to non-commercially available α- and β-amino acids used in this invention are widely described in the literature and well know to one skilled in the art. Suitable methods include, but are not limited to, those disclosed in the following references: (a) D. J. Ager et al., Current opinion in drug discovery & development 2001, 4, 800-807; (b) R. O. Duthaler Tetrahedron 1994, 50, 1539-1650; (c) M. J. O'Donnell Aldrichimica Acta 2001, 34, 3-15; (d) K. B. Sharpless et al., J. Am. Chem. Soc. 1998, 120, 1207-17; (e) E. Juaristi et al., Aldrichimica Acta 1994, 27, 3-11; (f) D. C. Cole Tetrahedron 1994, 50, 9517-9582 and references cited therein.

Compounds of the present invention in which A is —NH—CR¹R² in Formula I, can be made via the route shown in Scheme 3. Polystyrene aldehyde (PAL) resin was reductively aminated with a monoaryl diamine (NH₂CH₂CH₂NR⁹Ar) to obtain the resin 3A (Scheme 3). This material was acylated with an N-protected amino acid using standard conditions [as described in A. R. Chamberlin, Chem. Rev. 1997, 97, 2243-2266] and the product was then deprotected with piperidine to furnish 3B. After acylation with QCOCl under standard coupling condition, cleavage from resin using TFA furnished the urea 3C,

-   -   ii) TFA/DCM/H₂O.

An illustration of the compounds of the present invention in which A=—CR³H—O— in Formula I, is given in Scheme 4.

Methods for the synthesis of monosubstituted succinate derivatives are known in the art and are disclosed in a number of references including (a) D. A. Evans et al., J. Org. Chem. 1999, 64, 6411; (b) D. W. C. MacMillan et al., J. Am. Chem. Soc. 2001, 123, 2912; (c) S. Azam et al., J. Chem. Soc. Perkin Trans. 1 1996, 621; (d) A. Abell et al., Org. Lett. 2002, 4, 3663; (e) R. J. Cherney et al., Bioorg. Med. Chem. Lett. 2003, 13, 1297; (f) G. Shapiro et al., Tetrahedron Lett. 1992, 33, 2447; (g) N. J. S. Harmat et al., Tetrahedron Lett. 2000, 41, 1261. A representative procedure is outlined in Scheme 5 where acylation of an oxazolidinone chiral auxiliary with an acid chloride provides structure 5A. Alkylation of the corresponding enolate with t-butyl bromoacetate followed by LiOH/H₂O₂ mediated cleavage of the chiral auxiliary gives rise to the enantiomerically pure monosubstituted succinic acid monoester 5C.

Syn-2,3-disubstituted succinate derivatives can be accessed using the chemistry illustrated in Scheme 6, adapted from (a) M. J. Crimmin et al., Synlett 1993, 137; (b) C. Xue et al., J. Org. Chem. 2002, 67, 865 incorporated herein by references. Intermediate 5C was subjected to enolate formation using a 2.2 equivalent of a strong base followed by quenching with 1.5 equivalent of R³X (wherein X=OTf, I, Br and the like), providing exclusively the syn diastereomer 6A after chromatography.

Anti-2,3-disubstituted succinate derivatives can be obtained via selective inversion at the C-3 carbon center described by M. J. Crimmin et al., Synlett 1993, 137.

Alternatively, racemic succinic acid esters can be converted to enantiomerically enriched succinic acids via an enzyme catalyzed kinetic resolution, according to the procedures described by (a) H. Oikiwa et al., Tetrahedron Lett. 1996, 37, 6169; (b) B. Wirz et al., Tetrahedron: Asymmetry 1997, 8, 187 and references cited therein.

The compounds of the present invention in which A=—CHR³—CHR¹— in Formula I, can be prepared as illustrated in Scheme 7.

An illustration of the compounds of the present invention in which A=—O—CR³H— in formula I, is given in Scheme 8.

B. Preferred Compounds

Compounds that inhibit the cathepsin S activity can be found in U.S. Provisional Application Nos. 60/457,848 and 60/457,595, both filed Mar. 24, 2003, and 60/478,625 filed Jun. 13, 2003. The contents of each of the foregoing applications are incorporated herein by reference.

In one aspect, the present invention provides a compound of Formula I:

-   -   or a pharmaceutically acceptable salt or prodrug thereof,         wherein:     -   Q is a heterocycle selected from the group consisting of         azetidinyl, pyrrolidinyl, piperidyl, morpholinyl,         thiomorpholinyl, piperazinyl and indolinyl substituted with 0-2         R^(Q), wherein Q is connected to —C(═O)— via a ring nitrogen         atom; and NR²⁵R²⁶;     -   each R^(Q) is independently a member selected from the group         consisting of OH, F, Cl, —S(═O)₂CH₃—, acetyl, ═O, C₁-C₆ alkyl,         C₁-C₆ alkoxy, CF₃, OCF₃ and NR¹⁰R¹¹;     -   A is a member selected from the group consisting of —O—CR¹R²—,         —NH—CR¹R²—, —CR³R⁴—O—, and —CR³R⁴—CR¹R²—;     -   each of R¹ and R³ is independently a member selected from the         group consisting of H, a C₁-C₆ alkoxy, a C₁-C₆ alkyl substituted         with 0-2 R^(1a), wherein said C₁-C₆ alkyl may optionally contain         a heteroatom selected from the group consisting of —O—, —S—,         —S(═O)— and —S(═O)₂—; a C₂-C₆ alkenyl, a C₃-C₆ alkynyl, a C₃-C₇         cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl         substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³, a         5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-3 R¹³;     -   each R^(1a) is independently a member selected from the group         consisting of a C₆-C₁₀ aryl substituted with 0-3 R¹³, a 5- to         6-membered monocyclic or 8- to 10-membered bicyclic heteroaryl         containing 1 to 4 heteroatoms each independently a member         selected from the group consisting of N, O and S, wherein said         heteroaryl is substituted with 0-3 R¹³, a C₃-C₈ cycloalkyl         substituted with 0-2 R^(Q), a C₇-C₁₁ bicycloalkyl substituted         with 0-2 R^(Q), and a C₁-C₃ perfluoroalkyl;     -   each of R² and R⁴ is independently a member selected from the         group consisting of H, F, OH, C₁-C₆ alkyl and C₁-C₆ alkoxy;     -   R⁵ is a member selected from the group consisting of H,         C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, phenyl substituted with 0-2 R¹³, and a         5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-2 R¹³,         C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-2 R²¹, wherein         said C₁-C₆ alkyl may optionally contain a heteroatom selected         from the group consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and         —NR²²—;     -   each of R⁶, R⁷, R⁸ and R⁹ is independently a member selected         from the group consisting of H and C₁-C₆ alkyl;     -   alternatively, R⁵ and R⁷ are taken together to form a C₅-C₇         cycloalkyl, wherein a methylene of said C₅-C₇ cycloalkyl may         optionally be replaced with a heteroatom selected from the group         of —O—, —S—, —S(═O)—, and —S(═O)₂—;     -   each R¹⁰ is independently a member selected from the group         consisting of H, C₁-C₄ alkyl, (C₁-C₄ alkyl)-C(═O)— and (C₁-C₄         alkyl)-S(═O)₂—;     -   each R¹¹ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl;     -   each R¹² is independently a member selected from the group         consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3         R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms         each independently a member selected from the group consisting         of N, O and S, wherein said 5- to 6-membered heteroaryl is         substituted with 0-3 R¹³, and a C₁-C₆ alkyl substituted with 0-1         R¹⁹;     -   each R¹³ is independently a member selected from the group         consisting of H, OH, F, Cl, Br, CN, NO₂, COOR¹⁷, C(═O)NR¹⁷R¹⁸,         S(═O)₂NR¹⁷R¹⁸, acetyl, —SCH₃, —S(═O)CH₃, —S(═O)₂CH₃, —NR¹⁰R¹¹,         C₁-C₆ alkoxy, C₁-C₃ perfluoroalkyl, C₁-C₃ perfluoroalkoxy and a         C₁-C₆ alkyl;     -   each R¹⁴ is independently a member selected from the group         consisting of H, C₃-C₇ cycloalkyl, C₁-C₄ alkyl substituted with         0-1 R¹⁹, and a phenyl substituted with 0-3 R¹³;     -   each R¹⁵ is independently a member selected from the group         consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3         R¹³, and a C₁-C₆ alkyl substituted with 0-1 R¹⁹;     -   each R¹⁶ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl;     -   alternatively, R¹⁵ and R¹⁶ on the same N atom are taken together         to form a C₅-C₇ heterocycle containing 1-2 heteroatoms each         independently a member selected from the group consisting of N,         O and S;     -   each of R¹⁷ and R¹⁸ is independently a member selected from the         group consisting of H, C₁-C₄ alkyl and C₃-C₆ cycloalkyl;     -   each R¹⁹ is independently a member selected from the group         consisting of H, C₃-C₇ cycloalkyl, a phenyl substituted with 0-3         R¹³ and a 5- to 6-membered heteroaryl containing 1 to 4         heteroatoms each independently a member selected from the group         consisting of N, O and S, wherein said 5- to 6-membered         heteroaryl is substituted with 0-3 R¹³;     -   Ar is a member selected from the group consisting of phenyl         substituted with 0-3 R²⁰, and a 5- to 10-membered heteroaryl         containing 1 to 4 heteroatoms each independently a member         selected from the group consisting of N, O and S; wherein said         heteroaryl is substituted with 0-3 R²⁰;     -   each R²⁰ is independently a member selected from the group         consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃,         S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, —S(═O)₂NH(C═O)CH₃, C(═O)NR¹⁷R¹⁸,         CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃ and OCF₂H;     -   alternatively, R²⁰ and R⁹ are taken together to form a 5- to         7-membered heterocyclic ring containing 1-2 heteroatoms each         independently a member selected from the group consisting of N,         O and S; wherein said 5 to 7 membered heterocyclic ring is         ortho-fused to Ar; wherein said 5- to 7-membered heterocyclic         ring may be optionally substituted with 0-2 R²⁴;     -   each R²¹ is independently a member selected from the group         consisting of H, OH, F, Cl, CN, NO₂, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶,         NR²²R²³, C₁-C₃ perfluoroalkoxy, C₁-C₄ alkoxy, C₂-C₄ alkenyl,         C₂-C₄ alkynyl, phenyl substituted with 0-3 R¹³, a 5- to         6-membered heteroaryl containing 1 to 4 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heteroaryl is substituted with 0-3 R¹³,         C₃-C₈ heterocycle containing 1 to 2 heteroatoms each         independently a member selected from the group consisting of N,         O and S, wherein said heterocycle is substituted with 0-2 R¹³         and is saturated or partially unsaturated, and C₃-C₈ cycloalkyl;     -   R²² is independently a member selected from the group consisting         of H, ^(t)BOC, Cbz, C₃-C₈ cycloalkyl, (C₁-C₆ alkyl)-C(═O)—,         (C₁-C₆ alkyl)-S(═O)₂—, a C₁-C₆ alkyl substituted with 0-1 R¹⁹, a         phenyl substituted with 0-3 R¹³ and a 5- to 6-membered         heteroaryl containing 1 to 4 heteroatoms each independently a         member selected from the group consisting of N, O and S, wherein         said 5- to 6-membered heteroaryl is substituted with 0-3 R¹³;     -   each R²³ is independently a member selected from the group         consisting of H and C₁-C₄ alkyl.     -   each R²⁴ is independently a member selected from the group         consisting of C₁-C₄ alkyl, F, Cl and C₁-C₄ alkoxy, CF₃ and OCF₃;     -   alternatively, two R²⁴ may be combined to form C₃-C₆ cycloalkyl.     -   each of R²⁵ and R²⁶ is independently a member selected from the         group consisting of C₁-C₆ alkyl, wherein said C₁-C₆ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and —NR₂₂—.

Compounds of the present invention are cathepsin S inhibitors. In particularly preferred aspects, the cathepsin S inhibitors are non-inhibitory toward cathepsin K, L, B, or combinations thereof.

In a preferred aspect, the present invention provides a compound according to Formula Ia:

-   -   wherein:     -   R¹ is independently a member selected from the group consisting         of H, C₁-C₆ alkyl substituted with 0-1 R^(1a), wherein said         C₁-C₆ alkyl may optionally contain a heteroatom selected from         the group consisting of —O—, —S—, and —S(═O)₂, a C₂-C₆ alkenyl,         a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁         bicycloalkyl substituted with 0-2 R^(Q); phenyl substituted with         0-3 R¹³, a 5- to 6-membered heteroaryl containing 1 to 4         heteroatoms each independently a member selected from the group         consisting of N, O and S, wherein said heteroaryl is substituted         with 0-3 R¹³;     -   R⁴ is a member selected from the group consisting of H, F, OH         and C₁-C₆ alkyl.

In another preferred aspect, the present invention provides a compound according to Formula Ib:

-   -   wherein:     -   R¹ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄         alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S— and —S(═O)₂—;     -   R^(1a) is a member selected from the group consisting of a         phenyl substituted with 0-3 R¹³, a C₃-C₇ cycloalkyl substituted         with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2         R^(Q).

In yet another preferred aspect, the present invention provides a compound according to Formula Ic:

-   -   wherein:     -   R³ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄         alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S— and —S(═O)₂—; a C₃-C₇ cycloalkyl         substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl         substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³;     -   R^(1a) is a member selected from the group consisting of a         phenyl substituted with 0-3 R¹³, a C₃-C₇ cycloalkyl substituted         with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2         R^(Q).

In still yet another preferred aspect, the present invention provides a compound according to Formula Id:

-   -   wherein:     -   R¹ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄         alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S—, and —S(═O)₂;     -   R^(1a) is a member selected from the group consisting of a         phenyl substituted with 0-3 R¹³, C₃-C₇ cycloalkyl substituted         with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2         R^(Q);     -   R⁵ is a member selected from the group consisting of H, phenyl         substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl         substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S—, —S(═O)₂—;     -   each R²¹ is independently a member selected from the group         consisting of H, OH, F, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, phenyl         substituted with 0-3 R¹³, and C₃-C₇ cycloalkyl;     -   Ar is a member selected from the group consisting of phenyl         substituted with 0-3 R²⁰;     -   each R²⁰ is independently a member selected from the group         consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃,         S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, C(═O)NR¹⁷R¹⁸, CO₂R¹⁷, C(═NH)NH₂,         C₁-C₆ alkyl, CF₃, OCF₃;     -   alternatively, R²⁰ and R⁹ are taken together to form a 5         membered heterocyclic ring containing 1 nitrogen, wherein said 5         membered heterocyclic ring is ortho-fused to Ar; wherein said         5-membered heterocyclic ring may be optionally substituted with         0-2 R²⁴.

In another aspect, the present invention provides a compound according to Formula Ie:

-   -   wherein:     -   R² is H;     -   R³ are each independently selected from the group consisting of         H, C₁-C₆ alkyl, C₁-C₄ alkyl substituted with 1 R^(1a),     -   R^(1a) is a member selected from the group consisting of a C₃-C₇         cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl         substituted with 0-2 R^(Q);     -   R⁵ is a member selected from the group consisting of H, phenyl         substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl         substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may         optionally contain a heteroatom selected from the group         consisting of —O—, —S—, —S(═O)₂—;     -   each R²¹ is independently a member selected from the group         consisting of H, OH, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, phenyl         substituted with 0-3 R¹³, and C₃-C₈ cycloalkyl;     -   Ar is a member selected from the group consisting of phenyl         substituted with 0-3 R²⁰,     -   each R²⁰ is independently a member selected from the group         consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃,         S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, —S(═O)₂NH(C═O)CH₃, C(═O)NR¹⁷R¹⁸,         CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃ and OCF₂H;     -   alternatively, R²⁰ and R⁹ are taken together to form a         5-membered heterocyclic ring containing 1 nitrogen; wherein said         5-membered heterocyclic ring is ortho-fused to Ar; wherein said         5-membered heterocyclic ring may be optionally substituted with         0-2 R²⁴.

Q preferably has the following structures:

Preferred compounds of Formula I are set forth below:

-   1. Morpholine-4-carboxylic acid     (S)-2-cyclohexyl-1-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl     ester; -   2. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; -   3. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[2-(4-fluoro-phenylamino)-ethylcarbamoyl]-ethyl}-amide; -   4. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[2-(4-methoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide; -   5. [2-(4-Fluoro-phenylamino)-ethyl]-carbamic acid     1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   6.     2-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   7.     [4-(R)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-tetrahydro-furan-3-(R)-yl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   8.     2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   9.     3-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   10.     [1-(R)-Benzyloxymethyl-2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   11. Morpholine-4-carboxylic acid     2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylcarbamoyl]-ethyl     ester; -   12. Morpholine-4-carboxylic acid     2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylcarbamoyl]-ethyl     ester; -   13.     2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; -   14.     2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   15.     2-(R)-Cyclopentylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   16.     2-(R)-Cyclopentylmethyl-3-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   17. Morpholine-4-carboxylic acid     {2-benzylsulfanyl-1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide; -   18. Morpholine-4-carboxylic acid     {1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-2-phenylmethanesulfonyl-ethyl}-amide; -   19.     (R)-2-Cyclohexylmethyl-N-[2-(4-methoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   20.     2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; -   21.     2-(R)-(2-Cyclohexyl-ethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   22.     2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   23.     2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   24.     2-(R)-Cyclohexylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   25. 5,5-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic acid     [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide; -   26. 4,4-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-pentanoic acid     [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide; -   27.     2-(R)-Cyclopentylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; -   28.     2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   29.     2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(R)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   30. 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-5-phenyl-pentanoic acid     {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide; -   31.     2-(R)-Cyclopentylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   32.     N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-phenethyl-butyramide; -   33.     2-(R)-(2-Cyclopentyl-ethyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   34.     N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide; -   35.     2-(S)-Cyclohexyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   36.     2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   37.     2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   38.     4-Morpholin-4-yl-4-oxo-2-(R)-phenethyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   39.     4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; -   40.     4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; -   41.     2-(S)-(4-Fluoro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   42.     2-(S)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   43.     2-(R)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   44.     4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; -   45.     2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; -   46.     2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; -   47. 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-pent-4-enoic acid     {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide; -   48.     2-(S)-(4-Chloro-phenyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; -   49. (R)-5,5-Dimethyl-2-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic acid     [2-(5-methyl-isoxazol-3-ylamino)-ethyl]-amide; -   50.     2-(R)-Cyclohexylmethyl-4-(cis-2,6-dimethyl-morpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; -   51.     2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-4-thiomorpholin-4-yl-butyramide; -   52.     4-(4-Acetyl-piperazin-1-yl)-2-(R)-cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; -   53.     2-(S)-(4-Methoxy-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   54.     2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   55.     N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   56.     -{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-(4-trifluoromethyl-phenyl)-butyramide; -   57.     N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-p-tolyl-butyramide; -   58.     2-(R)-Cyclohexylmethyl-4-(1,1-dioxo-thiomorpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; -   59.     2-(R)-(3-Ethyl-3-hydroxy-cyclohexylmethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   60.     N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   61.     N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide; -   62.     2-(S)-(4-Fluoro-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   63.     2-(S)-(4-Methoxy-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; -   64.     2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(6-methoxy-pyridin-3-ylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   65.     N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(R)-spiro[2.5]oct-6-ylmethyl-butyramide; -   66.     N-[2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   67.     2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(3-methanesulfonyl-phenylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   68.     N-[1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   69.     N-[1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   70.     N-[1-(S)-Cyclopropyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -   71.     2-(R)-(4-methanesulfonyl-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; -   72. (S,S)-Morpholine-4-carboxylic acid     {2-cyclohexyl-1-[2-(4-methoxy-phenylamino)-1-methyl-ethylcarbamoyl]-ethyl}-amide; -   73. Morpholine-4-carboxylic acid     {2-cyclopentyl-1-(S)-[2-(4-methoxy-phenylamino)-1-(S)-methyl-ethylcarbamoyl]-ethyl}-amide; -   74. Morpholine-4-carboxylic acid     (2-cyclohexyl-1-(S)-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide; -   75. Morpholine-4-carboxylic acid (2-cyclohexyl-1     (S)-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide; -   76. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[2-methyl-1-(S)-(pyridin-3-ylaminomethyl)-propylcarbamoyl]-ethyl}-amide; -   77. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[1-(S)-methyl-2-(5-methyl-isoxazol-3-ylamino)-ethylcarbamoyl]-ethyl}-amide; -   78. Morpholine-4-carboxylic acid     {1-(S)-[2-(benzothiazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide; -   79. Morpholine-4-carboxylic acid     {1-(S)-[2-(benzooxazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide; -   80. (S,S)-Morpholine-4-carboxylic acid     {2-cyclohexyl-1-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethylcarbamoyl]-ethyl}-amide; -   81. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; -   82. Morpholine-4-carboxylic acid     {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-spirocyclopropyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; -   83. [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   84. [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic     acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl ester; -   85. [2-(4-Difluoromethoxy-phenylamino)-1-(S)-methyl-ethyl]-carbamic     acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl ester; -   86.     {2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   87.     {3-Phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   88. {1-(S)-[(4-Trifluoromethoxy-phenylamino)-methyl]-butyl}-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   89. [2-(4-Acetylsulfamoyl-phenylamino)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   90.     [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   91. [1-(S)-Methyl-2-(5-methyl-isoxazol-3-ylamino)-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   92.     (S,S)-[1-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl-methyl)-propyl]-carbamic     acid 1-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   93.     [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid     1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl     ester; -   94.     (S,S)-[2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethyl]-carbamic     acid 2-(4-acetyl-piperazin-1-yl)-1-cyclohexylmethyl-2-oxo-ethyl     ester; -   95.     [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid     1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl     ester; -   96.     [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl ester; -   97.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid     1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl     ester; -   98.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 2-(4-acetyl-piperazin-1-yl)-1-(S)-cyclohexylmethyl-2-oxo-ethyl     ester; -   99.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid     1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl     ester; -   100.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl ester; -   101.     [2-(5-Fluoro-3,3-spiro-cylopropyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   102.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-oxo-2-piperidin-1-yl-ethyl ester; -   103.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-oxo-2-pyrrolidin-1-yl-ethyl ester; -   104.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 2-cyclohexyl-1-(S)-dimethylcarbamoyl-ethyl ester; -   105.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid     1-(S)-cyclohexylmethyl-2-(1,1-dioxo-1λ⁶-thiomorpholin-4-yl)-2-oxo-ethyl     ester; -   106.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 2-cyclohexyl-1-(S)-[(2-methoxy-ethyl)-methyl-carbamoyl]-ethyl     ester; -   107.     [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic     acid 2-azetidin-1-yl-1-(S)-cyclohexylmethyl-2-oxo-ethyl ester; -   108.     [1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-carbamic     acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; -   109.     [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic     acid 2-morpholin-4-yl-2-oxo-1-(R,S)-phenyl-ethyl ester; -   110.     [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic     acid 2-morpholin-4-yl-2-oxo-1-(R)-phenylmethanesulfonylmethyl-ethyl     ester; -   111.     [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic     acid 1-(S)-cyclohexyl-2-morpholin-4-yl-2-oxo-ethyl ester.

Compounds of the present invention are either obtained in the free form, or as a salt thereof if salt forming groups are present, or as esters if ester forming groups are present.

Compounds of the present invention that have acidic groups can be converted into salts with pharmaceutically acceptable bases, e.g., an aqueous alkali metal hydroxide, advantageously in the presence of an ethereal or alcoholic solvent, such as a lower alkanol. Resulting salts can be converted into the free compounds, e.g., by treatment with acids. These, or other salts can also be used for purification of the compounds obtained. Ammonium salts are obtained by reaction with the appropriate amine, e.g., diethylamine, and the like.

In certain aspects, compounds of the present invention having basic groups can be converted into acid addition salts, especially pharmaceutically acceptable salts. These are formed, for example, with inorganic acids, such as mineral acids, for example, sulfuric acid, a phosphoric or hydrohalic acid, or with organic carboxylic acids, such as (C₁-C₄) alkane carboxylic acids which, for example, are unsubstituted or substituted by halogen, for example, acetic acid, such as saturated or unsaturated dicarboxylic acids, for example, oxalic, succinic, maleic or fumaric acid, such as hydroxycarboxylic acids, for example, glycolic, lactic, malic, tartaric or citric acid, such as amino acids, for example, aspartic or glutamic acid, or with organic sulfonic acids, such as (C₁-C₄)-alkylsuflonic acids (for example, methanesulfonic acid) or arylsulfonic acids which are unsubstituted or substituted (for example, by halogen). Preferred are salts formed with hydrochloric acid, methanesulfonic acid and maleic acid.

In view of the close relationship between the free compounds and the compounds in the form of their salts or esters, whenever a compound is referred to in this context, a corresponding salt or ester is also intended, provided such is possible or appropriate under the circumstances.

The compounds, including their salts, can also be obtained in the form of their hydrates, or include other solvents used for their crystallization.

The compounds of the present invention that comprise free hydroxyl groups may also exist in the form of pharmaceutically acceptable, physiologically cleavable esters, and as such are included within the scope of the invention. Such pharmaceutically acceptable esters are preferably prodrug ester derivatives, such being convertible by solvolysis or cleavage under physiological conditions to the corresponding compounds of the present invention which comprise free hydroxyl groups. Suitable pharmaceutically acceptable prodrug esters are those derived from a carboxylic acid, a carbonic acid monoester or a carbamic acid, preferably esters derived from an optionally substituted lower alkanoic acid or an arylcarboxylic acid.

As will be apparent to one of skill in the art, certain compounds of the present invention possess asymmetric carbon atoms (optical centers) or double bonds; the racemates, diastereomers, enantiomers, geometric isomers and individual isomers are all intended to be encompassed within the scope of the present invention.

The present invention provides compounds which inhibit cathepsin S selectively. In certain preferred aspects, the present invention provides compounds which selectively inhibit cathepsin S in the presence of cathepsin isozymes, such as cathepsin A, B, C, D, E, F, G, H, K, L, M, O, P, Q, R, V, W and X. In a more preferred aspect, the present invention provides compounds which selectively inhibit cathepsin S in the presence of cathepsin K, L, B, or combinations thereof.

Compounds of the present invention useful for treating cathepsin S dependent conditions, preferably have cathepsin S inhibition constants less than 10 μM. More preferably, compounds of the present invention useful for treating cathepsin S dependent conditions have cathepsin S inhibition constants of less than 1.0 μM. Most preferably, compounds of the present invention useful for treating cathepsin S dependent conditions have cathepsin S inhibition constants of less than 0.1 μM.

In a preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of a cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 10 times greater than their cathepsin S inhibition constant. In a more preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 100 times greater than their cathepsin S inhibition constant. In a most preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 1000 times greater than their cathepsin S inhibition constant.

IV. Compositions

The pharmaceutical compositions according to the invention are those suitable for enteral, such as oral or rectal, transdermal, topical, and parenteral administration to mammals, including humans, to inhibit cathepsin S activity, and for the treatment of cathepsin S dependent disorders, in particular chronic neuropathic pain (see, WO 03/020287), Alzheimer's disease and certain autoimmune disorders, including, but not limited to, juvenile onset diabetes, multiple sclerosis, pemphigus vulgaris, Graves' disease, myasthenia gravis, systemic lupus erythemotasus, rheumatoid arthritis and Hashimoto's thyroiditis; allergic disorders, including, but not limited to, asthma; and allogeneic immune responses, including, but not limited to, rejection of organ transplants or tissue grafts.

More particularly, the pharmaceutical compositions comprise an effective cathepsin S inhibiting amount of a compound of the present invention.

The pharmacologically active compounds of the present invention are useful in the manufacture of pharmaceutical compositions comprising an effective amount thereof in conjunction or mixture with excipients or carriers suitable for either enteral or parenteral application.

Preferred are tablets and gelatin capsules comprising the active ingredient together with a) diluents, e.g., lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and/or glycine; b) lubricants, e.g., silica, talcum, stearic acid, its magnesium or calcium salt and/or polyethyleneglycol; for tablets also c) binders, e.g., magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and or polyvinylpyrrolidone; if desired d) disintegrants, e.g., starches, agar, alginic acid or its sodium salt, or effervescent mixtures; and/or e) absorbents, colorants, flavors and sweeteners. Injectable compositions are preferably aqueous isotonic solutions or suspensions, and suppositories are preferably prepared from fatty emulsions or suspensions. The compositions may be sterilized and/or contain adjuvants, such as preserving, stabilizing, wetting or emulsifying agents, solution promoters, salts for regulating the osmotic pressure and/or buffers. In addition, they may also contain other therapeutically valuable substances. The compositions are prepared according to conventional mixing, granulating or coating methods, respectively, and contain about 0.1 to 75%, preferably about 1 to 50%, of the active ingredient.

Tablets may be either film coated or enteric coated according to methods known in the art.

Suitable formulations for transdermal application include an effective amount of a compound of the present invention with carrier. Preferred carriers include absorbable pharmacologically acceptable solvents to assist passage through the skin of the host. For example, transdermal devices are in the form of a bandage comprising a backing member, a reservoir containing the compound optionally with carriers, optionally a rate controlling barrier to deliver the compound to the skin of the host at a controlled and predetermined rate over a prolonged period of time, and means to secure the device to the skin. Matrix transdermal formulations may also be used.

Suitable formulations for topical application, e.g., to the skin and eyes, are preferably aqueous solutions, ointments, creams or gels well-known in the art. Such may contain solubilizers, stabilizers, tonicity enhancing agents, buffers and preservatives.

The pharmaceutical formulations contain an effective cathepsin S inhibiting amount of a compound of the present invention as defined above, either alone or in combination with another therapeutic agent.

In conjunction with another active ingredient, a compound of the present invention may be administered either simultaneously, before or after the other active ingredient, either separately by the same or different route of administration or together in the same pharmaceutical formulation.

The dosage of active compound administered is dependent on the species of warm-blooded animal (mammal), the body weight, age and individual condition, and on the form of administration. A unit dosage for oral administration to a mammal of about 50 to 70 kg may contain between about 5 and 500 mg of the active ingredient.

In a preferred aspect, the pharmaceutical composition of the present invention provides a compound according to Formula I.

In one aspect of the present invention, compositions of the present invention that comprise compounds of the present invention and pharmaceutically acceptable excipients, selectively inhibit cathepsin S in the presence of other cathepsin isozymes. In a more preferred aspect, the present invention provides compositions which selectively inhibit cathepsin S in the presence of cathepsin K, L, B, or combinations thereof.

In another aspect of the present invention, compositions of the present invention useful for treating cathepsin S dependent conditions, preferably have cathepsin S inhibition constants less than 10 μM. More preferably, compositions of the present invention useful for treating cathepsin S dependent conditions have cathepsin S inhibition constants of less than 1.0 μM. Most preferably, compositions of the present invention useful for treating cathepsin S dependent conditions have cathepsin S inhibition constants of less than 0.1 μM.

In a preferred aspect, compositions of the present invention utilize compounds that selectively inhibit cathepsin S in the presence of a cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 10 times greater than their cathepsin S inhibition constant. In a more preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 100 times greater than their cathepsin S inhibition constant. In a most preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 1000 times greater than their cathepsin S inhibition constant.

V. Methods

In view of their activity as inhibitors of cathepsin S, compounds of the present invention are particularly useful in mammals as agents for treatment and prophylaxis of diseases and medical conditions involving elevated levels of cathepsin S. For example, the compounds of the present invention are useful in treating Alzheimer's disease and certain autoimmune disorders, including, but not limited to juvenile onset diabetes, multiple sclerosis, pemphigus vulgaris, Graves' disease, myasthenia gravis, systemic lupus erythemotasus, rheumatoid arthritis and Hashimoto's thyroiditis; allergic disorders, including, but not limited to asthma; and allogeneic immune responses, including, but not limited to, rejection of organ transplants or tissue grafts.

Beneficial effects are evaluated in vitro and in vivo pharmacological tests generally known in the art, and as illustrated herein.

The above cited properties are demonstrable in vitro and in vivo tests, using advantageously mammals, e.g., rats, mice, dogs, rabbits, monkeys or isolated organs and tissues, as well as mammalian enzyme preparations, either natural or prepared by, e.g., recombinant technology. Compounds of the present invention can be applied in vitro in the form of solutions, e.g., preferably aqueous solutions or suspensions, and in vivo either enterally or parenterally, preferably orally, e.g., as a suspension or in aqueous solution, or as a solid capsule formulation. The dosage in vitro may range between about 10⁻⁵ molar and 10⁻⁹ molar concentrations. The dosage in vivo may range, depending on the route of administration, between about 0.1 and 100 mg/kg.

The antiarthritic efficacy of the compounds of the present invention for the treatment of rheumatoid arthritis can be determined using models such as, or similar to, the rat model of adjuvant arthritis, as described previously (R. E. Esser, et al., J. Rheumatology 1993, 20, 1176). The efficacy of the compounds of the present invention for the treatment of osteoarthritis can be determined using models such as, or similar to, the rabbit partial lateral meniscectomy model, as described previously (Colombo et al., Arth. Rheum. 1993, 26, 875-886). The efficacy of the compounds in the model can be quantified using histological scoring methods, as described previously (O'Byrne et al., Inflamm. Res. 1995, 44, S 177-S118).

The present invention also relates to methods of using compounds of the present invention and their pharmaceutically acceptable salts, or pharmaceutical compositions thereof, in mammals for inhibiting cathepsin S, and for the treatment of cathepsin S dependent conditions, such as the cathepsin S dependent conditions described herein, e.g., inflammation, rheumatoid arthritis and osteoarthritis.

In a preferred aspect, the present invention relates to a method of treating rheumatoid arthritis, osteoarthritis, and inflammation (and other diseases as identified above) in mammals comprising administering to a mammal in need thereof, a correspondingly effective amount of a compound of the present invention.

In a preferred aspect, the method of the present invention provides a compound according to Formula I.

Methods of the present invention useful for treating cathepsin S dependent conditions, preferably use compounds that have cathepsin S inhibition constants less than 10 μM. More preferably, methods of the present invention useful for treating cathepsin S dependent conditions use compounds that have cathepsin S inhibition constants of less than 1.0 μM. Most preferably, methods of the present invention useful for treating cathepsin S dependent conditions use compounds that have cathepsin S inhibition constants of less than 0.1 μM.

Moreover, the present invention relates to a method of selectively inhibiting cathepsin S activity in a mammal which comprises administering to a mammal in need thereof, an effective cathepsin S inhibiting amount of a compound of the present invention. In a preferred aspect, the methods of the present invention use compounds that selectively inhibit cathepsin S in the presence of a cathepsin isozyme, such as cathepsin A, B, C, D, E, F, G, H, K, L, M, O, P, Q, R, V, W and X. In a more preferred aspect, methods of the present invention use compounds that selectively inhibit cathepsin Sin the presence of cathepsin K, L, B, or combinations thereof.

In a preferred aspect, methods of the present invention use compounds that selectively inhibit cathepsin S in the presence of a cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 10 times greater than their cathepsin S inhibition constant. In a more preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 100 times greater than their cathepsin S inhibition constant. In a most preferred aspect, compounds of the present invention that selectively inhibit cathepsin S in the presence of cathepsin isozyme, have a cathepsin isozyme inhibition constant at least 1000 times greater than their cathepsin S inhibition constant.

VI. EXAMPLES

A. Compounds

General Procedure. All solvents stated as anhydrous were purchased that way from the manufacturer and used as received. All other purchased reagents were used as received. Unless otherwise stated, all reactions were carried out under a positive pressure of nitrogen. Silica gel chromatography was performed using pre-packed cartridges and an instrument for making a linear solvent gradient along with automated fraction collection. ¹H NMR spectral data were reported as follows: chemical shift on the δ scale (using residual protio solvent as the internal standard), multiplicity (s=singlet, d=doublet, t=triplet, q=quartet, m=multiplet), integration and coupling constant in hertz. ¹³C spectra were recorded as APT experiments and were reported in ppm with residual solvent for internal standard.

Reference 1. Synthesis of (S)-2-(4-Methoxy-phenylamino)-1-methyl Ethyl Amine

Step A: Preparation of (S)-2-(tert-Butoxycarbonylamino)-propionaldehyde. (S)-(−)-2-(tert-Butoxycarbonylamino)-1-propanol (523 mg, 2.98 mmol, 1.0 equiv.) was dissolved in 45 mL methylene chloride in a 100 mL r.b. flask with a magnetic stir bar. To this clear homogeneous solution, Dess-Martin periodinane (1.523 g, 3.591 mmol, 1.2 equiv.) was added in one portion and the cloudy white reaction mixture was allowed to stir at room temperature for 2 h. Thin-layer chromotography monitored the reaction to completion. The reaction mixture was diluted with 100 mL ethyl acetate. Sodium bisulfite solution (2 M, 20 mL) was added to the reaction mixture and the organic layer was separated. The aqueous layer was washed with 3×30 mL EtOAc. The combined organic layers were washed with 50 mL 1 M NaOH, followed by saturated NaCl (30 mL) and dried over MgSO₄. Filtration and rotary evaporation produced the desired product as a yellow oil (475 mg, 92% yield, R_(f)=0.63, 1:1 hexanes/ethyl acetate).

Step B: Preparation of [2-(4-methoxy-phenylamino)-(1S)-methyl-ethyl]-carbamic Acid tert-butyl Ester

(S)-2-(tert-Butoxycarbonylamino)-propionaldehyde (473 mg, 2.74 mmol) and p-anisidine (1.031 g, 8.371 mmol, 3.0 equiv.) was dissolved in 45 mL of MeOH at 0° C. in a 100 mL r.b. flask with a magnetic stir bar. Optionally, acetic acid (469 μL, 8.21 mmol, 3.0 equiv.) can be added via syringe to assist in the reaction. To the stirring dark colored solution was added sodium cyanoborohydride (326 mg, 5.82 mmol, 1.89 equiv.). Gas evolution and disappearance of color were observed. The reaction was allowed to slowly warm to room temperature with stirring over 30 minutes and the reaction was monitored by LC/MS. At the completion of the reaction, the mixture was quenched with 1 M NaOH, and extracted 3×50 mL ethyl acetate. The resulting organics were washed with 50 mL saturated NaHCO₃, 40 mL saturated NaCl, and dried over MgSO₄. Evaporation of ethyl acetate provided 728 mg of a brown oil. Purification by automated ISCO chromatography provided a clear oil of [2-(4-methoxy-phenylamino)-(1S)-methyl-ethyl]-carbamic acid tert-butyl ester (583 mg, 2.079 mmol, 76% yield). HPLC-MS calcd. for C₁₅H₂₄N₂O₃ (M+H⁺) 281.2, found 281.5. ¹H NMR (CDCl₃, 400 MHz) δ 1.21 (d, 6H, J=6.6 Hz), 1.47 (s, 9H), 3.05 (dd, 1H, J=12.2, 7.3 Hz), 3.13 (dd, 1H, J=12.2, 4.6 Hz), 3.76 (s, 3H), 3.93 (broad s, 1H), 4.62 (broad s, 1H), 6.60 (d, 2H, J=6.8 Hz), 6.80 (2H, d, J=6.8 Hz).

Step C: [2-(4-Methoxy-phenylamino)-(1S)-methyl-ethyl]-carbamic acid tert-butyl ester (383 mg, 1.37 mmol) was added to 10 mL of a trifluroacetic acid solution (10 v/v % in methylene chloride) at room temperature in a 25 mL r.b. flask with a magnetic stirbar. The reaction turns dark purple/black in color after 5 minutes. The reaction is allowed to stir at room temperature until the reaction is judged complete by HPLC/MS. The solvent is removed by evaporation and to provide 2-(4-Methoxy-phenylamino)-(1S)-methyl-ethyl-ammonium; trifluoro-acetate salt as a brown oil (394 mg, 1.34 mmol, 98% yield) and used directly in the next reaction. HPLC-MS calcd. for C₁₀H₁₆N₂O (M+H⁺) 181.1, found 181.5.

Reference 2. (R)-3-Benzyloxy-N¹-(4-methoxy-phenyl)-propane-1,2-diamine

Step A: N-Boc-OBn-Serine (750 mg, 2.54 mmol), p-anisidine (344 mg, 2.79 mmol) and HOBt (377 mg, 2.79 mmol) were charged to a 50 mL roundbottom flask and treated with CH₂Cl₂ (6 mL). The reaction was then treated with EDCI (535 mg, 2.79 mmol) and allowed to stir for 2 hours. The reaction was then diluted with ethyl acetate and extracted twice with water, twice with 1 M HCl and twice with 1 M NaOH. The organics were then dried over MgSO₄ and the solvent was removed to afford 450 mg (44%) of a white solid: ¹H NMR (CDCl₃, 400 MHz) δ 1.49 (s, 9H), 3.63-3.72 (m, 1H), 3.81 (s, 3H), 4.00-4.08 (m, 1H), 4.47-4.50 (m, 1H), 4.55-4.70 (m, 2H), 5.45-5.60 (m, 1H), 6.87 (d, 2H, J=8.8), 7.30-7.41 (m, 7H), 8.20-8.33 (m, 1H); HPLC-MS calcd. for C₂₂H₂₈N₂O₅ (M+H⁺) 401.2, found 401.4.

Step B: The product from Step A (400 mg, 1.00 mmol) was added to an ice cold solution of borane (1 M) in THF. The cooling bath was removed and the reaction was allowed to stir for 24 h at which point the excess reagent was quenched using 5% NaHSO₄. The reaction was diluted with ethyl acetate and extracted twice with 1 M NaOH. The organics were dried over MgSO₄ and the solvent was removed. The resulting residue contained material that was missing the Boc group and some material that still had it (by HPLC-MS). The oil was treated with MeOH (2 mL) and 4 M HCl (2 mL) and stirred for 3 hours. The solvent was then removed and the reaction was partitioned between ethyl acetate and 1 M NaOH. The aqueous phase was extracted twice more with ethyl acetate and the combined organics were dried over MgSO₄ and the solvent was removed.

Reference 3. Synthesis of(S)—N1-(4-trifluoromethoxy-phenyl)-propane-1,2-diamine Step A: (S)-2-(benzylcarbonylamino)-propionaldehyde.

(S)-2-(benzylcarbonylamino)-propanol (5 g, 23.9 mmol) was dissolved in CH₂Cl₂ (200 mL) and treated with Dess-Martin periodinane (12.26 g, 1.1 eq). The mixture was stirred for 2 hours, then quenched with sodium thiosulphate, and the solvent removed in vacuo. The residue was then separated between sodium hydroxide (1M, 500 mL) and ethyl acetate (500 mL). The organics were washed with brine, dried (MgSO₄) and evaporated in vacuo to yield a clear oil which was used immediately in the next step without further purification.

Step B: [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic Acid Benzyl Ester

(S)-2-(benzylcarbonylamino)-propionaldehyde was dissolved in methanol (300 mL). Acetic acid (4 mL, 2.9 eq) was added and the mixture treated with 4-trifluoromethoxy aniline (9.6 mL, 3 eq) and stirred for 15 minutes then sodium cyanoborohydride (4.36 g, 2.9 eq) was added with some effervescence. The mixture was stirred for 3 hours, and then the solvent reduced in vacuo. This was then separated between hydrochloric acid (1M, 500 mL×2) and ethyl acetate (500 mL). The organics were washed with sodium bicarbonate (500 mL), brine(500 mL), dried (MgSO₄) and evaporated in vacuo to give a clear oil which was purified by silica gel chromatography eluted with a gradient of 0-100% ethyl acetate/hexane.

Step C: (S)—N1-(4-Trifluoromethoxy-phenyl)-propane-1,2-diamine

[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic acid benzyl ester (23.9 mmol) was dissolved in ethanol (200 mL) then placed under nitrogen. 10% Palladium on carbon was added (0.5 g) and the mixture was stirred under hydrogen (atmospheric pressure) overnight. When reaction was complete, the mixture was filtered through celite. The celite was washed with ethanol (5×50 ml) then evaporated in vacuo to give a brown oil (4.03 g, 17.21 mmol, 72% yield over 3 steps).

Reference 4. Synthesis of 2,2-dimethyl-5-fluoroindoline

Step A: A solution of N-Boc-4-fluoroaniline (9.02 g, 42.7 mmol) in THF (112 mL) was cooled to −60° C. using a cryocool instrument. The solution was treated with 1.7 M t-BuLi in pentane (63 mL, 106.7 mmol) dropwise. After the first equivalent of base was consumed, a yellow solution formed. The reaction was allowed to warm to −20° C. and was stirred at that temperature for 2.5 hours. The reaction was then treated with a solution of methallyl bromide (5.67 g, 42.7 mmol) in THF (35 mL) dropwise and stirred for an additional 1.5 hours at −20° C. The reaction was then quenched by addition of water. After coming to room temperature, the reaction was treated with ethyl acetate and extracted with water and brine, dried over MgSO₄ and filtered. The solvent was then removed and the residue was purified on silica gel using a gradient of 0-25% ethyl acetate in hexane to afford 11.3 g (80% yield) of [4-Fluoro-2-(2-methyl-allyl)-phenyl]-carbamic acid tert-butyl ester as a white solid; ¹H NMR (CDCl₃, 400 MHz) δ 1.50 (s, 9H), 1.72 (s, 3H), 3.28 (s, 2H), 4.71 (s, 1H), 4.92 (s, 1H), 6.32-6.50 (m, 1H), 6.86 (dd, 1H, J₁, =3.0, J₂=9.1), 6.93 (ddd, 1H, J₁, =3.0, J₂=8.5, J₃=11.5), 7.65-7.82 (m, 1H); HPLC-MS calcd. for C₁₅H₂₀FNO₂ (M+H⁺-tBu) 210.1, found 210.3.

Step B: A sample of [4-Fluoro-2-(2-methyl-allyl)-phenyl]-carbamic acid tert-butyl ester (1.10 g, 4.14 mmol) was treated with anisole (5 mL), dichloromethane (5 mL) and trifluoroacetic acid (5 mL) and stirred for 4 hours. The solvent was removed and the reaction was transferred to a microwave reaction vial using methanesulfonic acid (3 mL). The reaction was heated to 170° C. for 10 minutes. The reaction was cooled to room temperature and quenched into excess stirring 1 M NaOH. The aqueous phase was extracted twice with ethyl acetate and the combined organics were dried over MgSO₄ and filtered. The resulting oil was purified on silica gel using a gradient of 0-70% t-butyl ethyl ether and hexane to afford 450 mg (66% yield) of 2,2-dimethyl-5-fluoroindoline; ¹H NMR (CDCl₃, 400 MHz) δ 1.08 (s, 6H), 2.58 (s, 2H), 6.24 (dd, 1H, J₁, =4.4, J₂=8.4), 6.43-6.48 (m, 1H), 6.53-6.56 (m, 1H); HPLC-MS calcd. for C₁₀H₁₂FN (M+H⁺) 166.1, found 166.4.

Reference 5. Synthesis of 3,3-dimethyl-5-fluoroindoline

According to the procedure described in S. Coulton et al. WO9925709 with the following modifications. N-(4-Fluoro-phenyl)-N-(2-methyl-allyl)-acetamide (5 grams, 24.12 mmol) was added to a microwave tube with aluminum trichloride (7 grams, 52.4 mmol). The tube was capped and heated to 150° C. for 20 minutes under microwave. The slurry was worked up with water and ethyl acetate, the organic layer was extracted with 3 washes of saturated sodium bicarbonate solution and the organic layer was dried over magnesium sulfate. The solution was then filtered and rotary evaporated to yield pure 1-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethanone in quantitative yield. This was converted to the free indoline by suspending the entire 5 grams of product in 20 mL of 6 M HCl and heating in a microwave to 200° C. for 10 minutes. The resulting 5-Fluoro-3,3-dimethyl-2,3-dihydro-1H-indole crystallized on cooling as the hydrochloride salt in quantitative yield. This material was identical to the previously reported compound.

Reference 6. Synthesis of (S)-[1-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-carbamic Acid Benzyl Ester

Step A: (S)-cyclopropyl glycine was prepared according to a modified procedure from that reported in D. J. Bayston et al. U.S. Pat. No. 6,191,306. A sample of (R)-phenethyl-(S)-cyclopropyl glycine (16.8 g, 76.7 mmol) was treated with THF (200 mL), water (100 mL) and 10% Pd/C (4.76 g). To the stirring mixture was added formic acid (17 mL) and the reaction was stirred overnight. The catalyst was then removed by filtration through a pad of celite and the solvent was removed by rotary evaporation. The material was co-evaporated with methanol several times and dried under vacuum to afford 4.75 g (54% yield) of the desired material as a solid which was used without further purification.

The material from the previous step (4.75 g, 41 mmol) was dissolved in 130 mL of 1 N NaOH and treated with benzyl chloroformate (5.92 g, 49.5 mmol) with vigorous stirring. The reaction was stirred overnight and then extracted with dichloromethane twice. The organics were discarded and the aqueous phase was acidified with conc. HCl and extracted with dichloromethane three times. The combined organics were dried over MgSO₄ and the solvent was removed to afford 7.38 g (72% yield) of the (S)-benzyloxycarbonylamino-cyclopropyl-acetic acid as a white solid.

Step B: A solution of (S)-benzyloxycarbonylamino-cyclopropyl-acetic acid (3.2 g, 12.8 mmol) in THF (20 mL) was cooled in an ice/water bath and treated with a 1 M solution of BH₃ in THF (16.7 mL, 16.7 mmol). The reaction was stirred for 4 hours and then treated with 1 M HCl until the bubbling ceased. The reaction was stirred overnight and the organic solvent was removed by rotary evaporation. The residue was treated with ethyl acetate and transferred to a separatory funnel. The aqueous phase was discarded and the organics were washed twice with 1 M NaOH, dried over MgSO₄ and the solvent was removed. The residue was purified on silica gel using a gradient of 0-100% ethyl acetate in hexane to afford 1.5 g (50% yield) of (S)-(1-Cyclopropyl-2-hydroxy-ethyl)-carbamic acid benzyl ester as a white solid; ¹H NMR (CDCl₃, 400 MHz) δ 0.26-0.37 (m, 1H), 0.34-0.44 (m, 1H), 0.47-0.61 (m, 2H), 0.83-0.94 (m, 1H), 2.95-3.04 (m, 1H), 3.70 (dd, 1H, J₁=5.8, J₂=11.1), 3.79-3.88 (m, 1H), 5.00-5.12 (m, 1H), 5.10 (s, 2H), 7.29-7.31 (m, 5H); HPLC-MS calcd. for C₁₃H₁₇NO₃ (M+H⁺) 236.1, found 236.3.

Step C: (S)-[1-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-carbamic acid benzyl ester was prepared in 67% yield an analogous manner to reference 3 except that the alcohol from the previous step and 1 equivalent of 3,3-dimethyl-5-fluoroindoline (WO 9925709) were used as coupling partners; HPLC-MS calcd. for C₂₃H₂₇FN₂O₂ (M+H⁺) 383.2, found 383.4.

Reference 7. Synthesis of 5-fluoro-3,3-spirocyclopropyl-indoline

Step A: A solution of 5-fluoroisatin (5 g, 30.2 mmol) in DMF (60 mL) was cooled in an ice/water bath and treated with sodium hydride (1.44 g, 60.6 mmol) portionwise. The reaction was stirred for 15 minutes after the addition of the last portion and then treated with p-methoxybenzyl chloride (5.32 g, 45.3 mmol) and allowed to stir for 1 hour. The reaction was then quenched by slow addition of excess methanol. After bubbling had stopped, the reaction was poured into water (100 mL) and extracted twice with ethyl acetate. The organics were combined, dried over MgSO₄ and the solvent was removed. The residue was purified by silica gel chromatography using a gradient of 0-100% ethyl acetate in hexane to afford 7.1 g (82%) of 5-Fluoro-1-(4-methoxy-benzyl)-1H-indole-2,3-dione; ¹H NMR (CDCl₃, 400 MHz) δ 3.79 (s, 3H), 4.86 (s, 2H), 6.75 (dd, 1H, J₁=3.6, J₂=8.6), 6.84-6.90 (m, 2H), 7.19 (ddd, 1H, J₁=J₂=8.6, J₃=3.6), 7.22-7.27 (m, 1H), 7.26-7.31 (m, 2H); HPLC-MS calcd. for C₁₆H₁₂FNO₃ (M+H⁺) 286.1, found 286.3.

Step B: A solution of 5-fluoro-1-(4-methoxy-benzyl)-1H-indole-2,3-dione (7.1 g, 24.9 mmol) in hydrazine hydrate (35 mL) and ethanol (15 mL) was refluxed overnight, diluted with water and extracted twice with ethyl acetate. The combined organics were dried over Na₂SO₄, filtered and concentrated. The residue was purified by silica gel chromatography using a gradient of 0-100% ethyl acetate in hexane to afford 6.1 g (90%) of 5-fluoro-1-(4-methoxy-benzyl)-1,3-dihydro-indol-2-one; ¹H NMR (CDCl₃, 400 MHz) δ 3.59 (s, 2H), 3.77 (s, 3H), 4.83 (s, 2H), 6.63 (dd, 1H, J₁=4.2, J₂=8.6), 6.82-6.91 (m, 3H), 6.96-7.01 (m, 1H), 7.19-7.23 (m, 1H), 7.27-7.31 (m, 1H); HPLC-MS calcd. for C₁₆H₁₄FNO₂ (M+H⁺) 272.1, found 272.3.

Step C: A solution of 5-fluoro-1-(4-methoxy-benzyl)-1,3-dihydro-indol-2-one (6.12 g, 22.6 mmol) in DMF (65 mL) was cooled in an ice/water bath and treated with dibromoethane (6.35 g, 33.8 mmol) followed by sodium hydride (1.09 g, 45 mmol) portionwise. After stirring at 0° C. for 1 hour, the reaction was cooled to −78° C. and treated with excess methanol. After bubbling had stopped, the reaction was poured into water (100 mL) and extracted twice with ethyl acetate. The organics were combined, dried over Na₂SO₄ and the solvent was removed. The residue was purified by silica gel chromatography using a gradient of 0-100% ethyl acetate in hexane to afford 4.1 g (61%) of 5-fluoro-1-(4-methoxy-benzyl)-siprocyclopropyloxindole; ¹H NMR (CDCl₃, 400 MHz) δ 1.54 (dd, 2H, J₁=4.0, J₂=7.8), 1.83 (dd, 2H, J₁=4.3, J₂=8.1), 3.77 (s, 3H), 4.91 (s, 2H), 6.57 (dd, 1H, J₁=2.5, J₂=8.0), 6.69 (dd, 1H, J₁=4.2, J₂=8.5), 6.81 (dd, 1H, J₁=2.5, J₂=9.3), 6.83-6.87 (m, 2H), 7.22-7.25 (m, 2H); HPLC-MS calcd. for C₁₈H₁₆FNO₂ (M+H⁺) 298.1, found 298.3.

Step D: A solution of 5-fluoro-1-(4-methoxy-benzyl)-siprocyclopropyloxindole (3.38 g, 11.4 mmol) in TFA (20 mL) was stirred at 60° C. overnight. The solvent was then removed and the reaction was diluted with ethyl acetate and washed with saturated aqueous NaHCO₃ until the washings were neutral. The organic phase was then washe with brine, dried over Na₂SO₄ and the solvent was removed. The residue was purified by silica gel chromatography using a gradient of 0-100% ethyl acetate in hexane to afford 1.94 g (96%) of 5-fluoro-siprocyclopropyloxindole; ¹H NMR (MeOD, 400 MHz) δ 1.76-1.86 (m, 4H), 6.91-6.94 (m, 1H), 7.07-7.11 (m, 2H); HPLC-MS calcd. for C₁₀H₈FNO (M+H⁺) 178.2, found 178.3.

Step E: A sample of 5-fluoro-siprocyclopropyloxindole (172 mg, 97 μmol) was cooled in an ice/water bath and treated with a 1.0 M solution of LAH (1.94 ml, 1.9 mmol). The reaction was stirred at room temperature for 15 minutes and then at 50° C. for 3 hours and finally was cooled back down with an ice/water bath. The reaction was treated with 1 M NaOH (1.9 mL) followed by water (1.9 mL). The reaction was filtered over celite and dried over MgSO₄. After filtration, the solvent was removed and the crude material of 5-fluoro-siprocyclopropylindoline was used without purification.

In addition, synthesis of other 3,3-spiro-cycloalkylindolines are also described in (1) Jackson, A. H. et al. Tetrahedron (1968), 24(1), 403-13; (2) Jansen, A. B. A. et al. Tetrahedron (1965), 21(6), 1327-31; (3) Bermudez, J. et al. J. Med. Chem. (1990), 33(7), 1929-32; (4) Nishio, T. et al. Helv. Chim. Acta (1990), 73(6), 1719-23; (5) Nishio, T. et al. J. Chem. Soc., Perkin Trans 1 (1991), (1), 141-3; (6) Kucerovy, A. et al. Synth. Commun. (1992), 22(5), 729-33; (7) Kato, M. et al. Chem. Pharm. Bull.(1995), 43(8), 1351-7.

Reference 8. Synthesis of 2,2,5-trifluoroindoline

Step A: 5-Fluoro-1H-indole-2,3-dione (956 mg, 5.79 mmol, 1 eq) was added as a solution in dry DMF to a stirred slurry of sodium hydride (278 mg, 11.6 mmol, 2 eq) in dry DMF drop wise over 15 minutes under an inert atmosphere with adequate pressure release to accommodate H₂ evolution. The resulting mixture was stirred for 1 hour and p-methoxybenzyl chloride was added via syringe to the reaction. The solution was then stirred ca 2 hours and worked up by addition of water followed by extraction into ethyl acetate. The organic layer was washed twice with water and then dried over MgSO₄. Column chromatography with ethyl acetate/hexane afforded 5-Fluoro-1-(4-methoxy-benzyl)-1H-indole-2,3-dione as a red solid (1.3 g, 80% yield). ¹H NMR (CDCl₃) δ (ppm): 7.3-7.24 (m, 3H), 7.20 (td, J=8.7, 2.7 Hz, 1H), 6.9-6.86 (m, 2H), 6.76 (dd, J=8.6, 3.6 Hz, 1H), 3.81 (s, 2H), 3.78 (s, 3H). LC/MS=286.1 (M+1).

Step B: The product from step A (200 mg, 0.701 mmol, 1 eq) was dissolved in 10 mL of dry DCM and placed under and inert atmosphere. DAST (339 mg, 2.103 mmol, 3 eq) was added via syringe and the reaction was stirred overnight. The reaction was worked up by addition of saturated aqueous sodium bicarbonate and the organic layer was dried over MgSO₄, filtered, and rotary evaporated to dryness. The resulting crude material was purified by flash chromatography using ethyl acetate/hexane as a solvent system. ¹H NMR (CDCl₃) δ (ppm): 7.3-7.28 (m, 1H), 7.22 (d, J=8.7 Hz, 2H), 7.09 (td, J=8.7, 1.3 Hz, 1H), 6.87 (d, J=8.7 Hz, 2H), 6.73 (m, 1H), 4.83 (s, 2H), 3.79 (s, 3H). LC/MS=308.1(M+1).

Step C: The product from step B (1.178 g, 3.83 mmol, 1 eq) was dissolved in 75 mL of dry THF and placed under an inert atmosphere. LiAlH₄ (291 mg, 7.66 mmol, 2 eq) was added as a solid under a positive pressure of N₂ at −78° C. The reaction was allowed to stir at this temperature for 30 min and then allowed to warm to room temp over a period of 6 hours. The reaction was worked up by addition of water dropwise followed by 4 equivalents of aqueous KOH. The slurry was diluted with 500 mL of water and extracted with 2×200 mL portions of ethyl acetate. The organic layers were combined, dried over MgSO₄, filtered, and rotary evaporated to dryness. The resulting crude material was purified by flash chromatography using ethyl acetate/hexane as a solvent system yielding 320 mg of pure material (28%). ¹H NMR (CD₃OD) δ (ppm): 7.21 (d, J=8.8 Hz, 2H), 7.06 (dd, J=8.2, 1.3 Hz, 1H), 6.89 (m, 1H), 6.84 (d, J=8.7 Hz, 2H), 6.77 (dd, J=8.6, 4.3 Hz, 1H), 4.83 (s, 2H), 3.73 (s, 3H), 3.12 (s, 2H). LC/MS=294.1(M+1).

Step D: The product from step C (50 mg, 0.1704 mmol, 1 eq) was taken up in 1 mL of TFA. The solution was placed in a microwave tube, sealed, and heated to 175° C. for 5 minutes. The resulting black solution was neutralized with saturated sodium bicarbonate and extracted with 2×50 mL portions of ethyl acetate. The organic layers were dried over MgSO₄, filtered, and rotary evaporated to dryness. The resulting solid was dissolved in a 50:50 mix of DMSO/MeOH and purified by prep HPLC. Yield 23.8 mg of white solid (81%). ¹H NMR (DMSO D₆) δ (ppm): 10.41 (s, 1H), 7.13 (dd, J=8.6, 2.4 Hz, 1H), 7.01 (td, J=8.6, 2.7 Hz, 1H), 6.8 (dd, J=8.5, 4.5 Hz, 1H), 3.5 (s, 2H).

Example 1 Morpholine-4-carboxylic Acid (S)-2-cyclohexyl-1-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl Ester

To a stirring suspension of L-cyclohexylalanine (4.00 g, 23.4 mmol) in 0.5M H₂SO₄ (120 mL) at 0° C. was slowly added dropwise an aqueous solution of NaNO₂ (12.1 g in 40 mL H₂O). Addition was complete after approximately 1 h, at which point the solution was allowed to warm to room temperature. After 16 h, the reaction mixture was extracted with ether (3×100 mL), and the combined organic extracts were washed with 1M NaHSO₄ (1×200 mL) and brine (1×100 mL) and then dried over anhydrous Na₂SO₄. The solvent was removed in vacuo, and the crude product was recrystallized from Et₂O/pentane (10 mL/100 mL) to afford 2.1 g (52% yield) of (S)-cyclohexyl lactic acid as fine white needles.

To a stirring suspension of (S)-cyclohexyl lactic acid (558 mg, 3.42 mmol) in CH₂Cl₂ was added 2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylamine (616 mg, 3.42 mmol), HATU (1.429 g, 3.76 mmol), and DIEA (1.79 mL, 10.3 mmol). The reaction mixture was stirred at room temperature for several hours until the starting material had disappeared by LCMS. Ethyl acetate (100 mL) was added and the solution was washed with 1M NaHSO₄ (2×100 mL), sat'd aq NaHCO₃ (2×100 mL) and brine (1×100 mL). The solvent was removed in vacuo and the crude material was purified by silica gel chromatography (hexanes/EtOAc) to afford 320 mg of 3-Cyclohexyl-N-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethyl]-2-(S)-hydroxy-propionamide as a white powder.

To a stirring solution of 3-Cyclohexyl-N-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethyl]-2-(S)-hydroxy-propionamide (263 mg, 0.79 mmol) in CH₂Cl₂ (2.0 mL) was added pyridine (0.1 mL) and 4-nitrophenyl chloroformate (202 mg, 1.01 mmol). The reaction was stirred at room temperature overnight at which point the starting material had disappeared by LCMS. The crude material was purified by silica gel chromatography to afford the corresponding mixed carbonate as a white powder.

The resulting carbonate (50 mg, 0.094 mmol) was dissolved in CH₂Cl₂ (1.0 mL), excess morpholine (0.1 mL) was added and the reaction was stirred at room temperature for several until the starting material had disappeared by LCMS. The solvent was removed in vacuo and the crude material was purified by reverse phase HPLC. HPLC-MS calcd. for C₂₄H₃₄FN₃O₄ (M+H⁺) 448.25, found 448.5.

Example 2 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide

The title compound was synthesized according to the procedure outlined in Example 4 starting from 2-(5-Fluoro-2,3-dihydro-indol-1-yl)-ethylamine.

HPLC-MS calcd. for C₂₄H₃₅FN₄O₃ (M+H⁺) 447.27, found 447.5.

Example 3 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[2-(4-fluoro-phenylamino)-ethylcarbamoyl]-ethyl}-amide

The title compound was synthesized according to the procedure outlined in Example 4 starting from N1-(4-fluorophenyl)-ethane-1,2-diamine.

HPLC-MS calcd. for C₂₂H₃₃FN₄O₃ (M+H⁺) 421.25, found 421.5.

Example 4 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[2-(4-methoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide

Step A: An aldehyde-functionalized polystyene resin (“Pal-Resin”, 16.76 g @1.05 mmol/g, 17.6 mmol) was swelled in DMF (50 ml) for 10 min. N1-(4-Methoxy-phenyl)-ethane-1,2-diamine (5.85 g, 35 mmol, prepared according Scheme 1) in DMF (150 mL) was added followed by acetic acid (8.1 mL, 8 eq), and the mixture was agitated for 1 hour at room temperature. Sodium triacetoxyborohydride (11.2 g, 52.8 mmol eq.) is then added and the mixture was shaken for 16 hours at room temperature. The reductively aminated resin was then filtered and washed (DMF×3, equal mixture of methanol/dichloromethane×4, Acetonitrile×3).

Step B: The resin (17.6 mmol) is swelled in dimethylformamide (50 mL) and a solution of Fmoc-CHA-OH(20.45 g, 3 eq), HOBt (8.08 g, 3 eq) and DIC (4.58 mL, 3 eq) was added. The mixture was shaken for 3 hours then washed (dimethylformamide×3, equal mixture of methanol/dichloromethane×4, Acetonitrile×3).

Synthesis of final products is performed on an Argonaut Quest 210 with automated washing module performing washes automatically.

Step C: Fmoc Deprotection.

The resin (250 mg, 0.163 mmol) is weighed into reaction vessels followed by a stirrer bar then treated with piperidine in dimethylformamide (4 mL of a 20% solution) and the mixture agitated for one hour. The resin is then washed (3×dimethylformamide, 3×dichloromethane).

Step D: Urea Formation.

A solution morpholine carbonyl chloride (3 eq, 0.490 mmol) dissolved in dichloromethane (10 mL) was added to the resin (0.163 mmol). DIEA (3 eq, 0.490 mmol) was added, and the reaction is agitated for three hours then washed with dimethylformamide four times, dichloromethane four times, then dried with nitrogen.

Step E: Cleavage.

The resin is treated with a mixture of trifluoroacetic acid, dichloromethane and water (45:45:10, 10 mL). It is agitated for one hour then retreated, agitated for five minutes, then washed one more time and filtered into vials. The solvent is evaporated in vacuo then purified using a Waters mass directed LCMS system (7.5 min method, gradient 10-90% acetonitrile/water with 0.35% trifluoroacetic acid). The compounds are then analyzed then concentrated to a solid via lyophilization.

HPLC-MS calcd. for C₂₃H₃₆FN₄O₄ (M+H⁺) 433.27, found 433.5.

Example 5 [2-(4-Fluoro-phenylamino)-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

To a stirring suspension of L-cyclohexylalanine (4.00 g, 23.4 mmol) in 0.5M H₂SO₄ (120 mL) at 0° C. was slowly added dropwise an aqueous solution of NaNO₂ (12.1 g in 40 mL H₂O). Addition was complete after approximately 1 h, at which point the solution was allowed to warm to room temperature. After 16 h, the reaction mixture was extracted with ether (3×100 mL), and the combined organic extracts were washed with 1M NaHSO₄ (1×200 mL) and brine (1×100 mL) and then dried over anhydrous Na₂SO₄. The solvent was removed in vacuo, and the crude product was recrystallized from Et₂O/pentane (10 mL/100 mL) to afford 2.1 g (52% yield) of (S)-cyclohexyl lactic acid as fine white needles.

To a stirring solution of (S)-cyclohexyl lactic acid (300 mg, 1.74 mmol), morpholine (0.15 mL, 1.74 mmol), and DIEA (0.91 mL, 5.23 mmol) in CH₂Cl₂ (3 mL) was added HATU (728 mmol, 1.92 mmol) and the reaction mixture was stirred at room temperature overnight. EtOAc (100 mL) was added and the solution was washed with sat'd NaHCO₃ (2×100 mL), brine (1×100 mL), dried over Na₂SO₄, and concentrated in vacuo to afford the corresponding amide as a colorless oil which was used without purification.

The resulting amide (1.74 mmol) was dissolved in pyridine (5 mL) and 4-nitrophenyl chloroformate (405 mg, 2.21 mmol) was added. The reaction mixture was stirred at 70° C. for 4 h at which point the starting material had disappeared by LCMS. The reaction was then cooled to room temperature, EtOAC (100 mL) was added, the organic layer was washed with 1M NaHSO₄, and dried over Na₂SO₄. The crude material was purified by silica gel chromatography (hexanes/EtOAc) to afford 550 mg (78% yield, over two steps) of the nitrophenyl carbonate as a white powder.

To a stirring suspension of N1-(4-fluorophenyl)-ethane-1,2-diamine.2HCl (110 mg, 0.48 mmol) and DIEA (0.34 mL, 1.93 mmol) in THF (2.0 mL) was added nitrophenyl carbonate obtained in the last step (196 mg, 0.48 mmol). The reaction mixture was stirred at room temperature, and after 18 h the starting material had disappeared by LCMS. Solvent was evaporated and the crude material was purified by silica gel chromatography (Hexanes/EtOAc) followed by a second purification by reverse phase HPLC to afford the title compound of example 5 as a white powder (55 mg, 27% yield).

HPLC-MS calcd. for C₂₂H₃₂FN₃O₄ (M+H⁺) 422.24, found 422.5.

Example 6 2-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

(R)-2-(Cyclohexylmethyl)succinic acid-1-methyl ester (470 mg, 2.06 mmol) (Acros Organics) was treated with morpholine (350 mg, 4.00 mmol, 2 eq.) and HATU (745 mg, 2.26 mmol). The reagents were dissolved in dry dichloromethane (5 mL) and treated with diisopropylethyl-amine (1 mL, 5.741 mmol). The reaction was allowed to stir overnight. The reaction was monitored by LC/MS and the reaction directly purified by prep-LC/MS. Product was obtained as a clear oil (460 mg, 1.54 mmol, 75%). This resulting product (460 mg, 1.54 mmol) was dissolved in a 2:1 mixture of MeOH (10 mL) and H₂O (5 mL) and placed in a 0° C. ice bath. Lithium hydroxide (45 mg, 1.87 mmol, 1.2 eq.) was added in one portion and allowed to stir for 8 hours, slowly warming to 23° C. After the reaction was completed, methanol was removed by evaporation. Ethyl acetate (75 mL) was added and the solution was extracted with 1M HCl (50 mL). The aqueous phase was extracted 2×75 mL of ethyl acetate and the combined organic phases were washed with saturated sodium bicarbonate (50 mL), saturated sodium chloride (50 mL) dried over magnesium sulfate, filtered and evaporated to provide 220 mg of product as an yellow oil (0.77 mmol, 50% yield). A portion of the resulting product (220 mg, 0.77 mmol), N1-(4-Fluoro-phenyl)-ethane-1,2-diamine (193 mg, 0.85 mmol, 1.1 eq. prepared according to Scheme 1) and HATU (280 mg, 0.85 mmol, 1.1 eq.) were dissolved in dry dichloromethane (4 mL) and treated with diisopropylethylamine (400 μL, 5.741 mmol). The reaction mixture was allowed to stir overnight and monitored by LC/MS. The reaction mixture was directly purified by prep-LC/MS and provided 56 mg (0.10 mmol, 13%) of the title compound as a white solid: ¹H NMR (CDCl₃, 400 MHz) δ 0.74-1.96 (m, 13H), 2.42-2.98 (m, 3H), 3.21-3.98 (m, 12H), 7.11-7.36 (m, 4H); HPLC-MS calcd. for C₂₃H₃₄FN₃O₃ (M+H⁺) 420.5, found 420.5.

Example 7 [4-(R)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-tetrahydro-furan-3-(R)-yl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

Step A: Synthesis of 1-(4-(R)-Azido-tetrahydro-furan-3-(R)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole and 1-(4-(R)-Azido-tetrahydro-furan-3-(S)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole.

A sample of 3-(R)-azido-4-(R)-hydroxytetrahydrofuran (232 mg, 1.8 mmol) was dissolved in dichloromethane (10 mL), cooled in an ice/water bath and treated with the Dess-Martin periodinane (917 mg, 2.2 mmol). The reaction was allowed to warm to room temperature and stirred for 1 hour, at which point TLC analysis indicated that the reaction was over. The resulting solution of the ketone was then treated with a solution of 5-fluoro-3,3-dimethylindoline (328 mg, 2.0 mmol) and acetic acid (148 mg, 2.7 mmol) in methanol (10 mL) and THF (5 mL). The reaction was then treated with sodium cyanoborohydride (170 mg, 2.7 mmol) and stirred overnight. The volatiles were then removed in vacuo and the reaction was picked up in ethyl acetate and extracted with 1 M NaOH. The organics were dried over Na₂SO₄ and the solvent was removed. The residue was purified by silica gel chromatography using a linear gradient of 0-50% ethyl acetate in hexane to afford 155 mg (31%) of the trans isomer and 50 mg (10%) of the trans isomer.

1-(4-(R)-Azido-tetrahydro-furan-3-(R)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole: ¹H NMR (400 MHz, CDCl₃) δ 6.77 (m, 2H), 6.38 (dd, J=6.0, 4.0 Hz, 1H), 4.31 (m, 1H), 4.09 (dd, J=10.0, 5.8 Hz, 1H), 4.03 (m, 3H), 3.83 (dd, J=10.0, 3.2 Hz, 1H), 3.45 (d, J=8.2 Hz, 1H), 3.26 (d, J=8.2 Hz, 1H), 1.32 (s, 3H), 1.31 (s, 3H); HPLC-MS calcd. for C₁₄H₁₇FN₄O₂ (M+H⁺) 277.3, found 277.4.

1-(4-(R)-Azido-tetrahydro-furan-3-(S)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole: ¹H NMR (400 MHz, CDCl₃) δ 6.77 (m, 2H), 6.44 (dd, J=8.5, 4.0 Hz, 1H), 4.11 (m, 2H), 4.02 (m, 3H), 3.75 (dd, J=8.6, 2.6 Hz, 1H), 3.23 (d, J=8.3 Hz, 1H), 3.07 (d, J=8.4 Hz, 1H), 1.29 (s, 3H), 1.27 (s, 3H); HPLC-MS calcd. for C₁₄H₁₇FN₄O₂ (M+H⁺) 277.3, found 277.4.

Step B. A sample of 1-(4-(R)-Azido-tetrahydro-furan-3-(R)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole (55 mg, 0.20 mmol) was treated with methanol (5 mL) and PtO₂ (2.4 mg, 0.01 mmol). A stream of hydrogen was bubbled though the reaction for 5 minutes and the reaction was stirred under a balloon pressure of hydrogen for 3 hours. The atmosphere in the reaction was switched back to nitrogen and the reaction was filtered through a bed of celite. The solvent was removed and the residue was removed and the resulting material was dried on the high vac for an hour. The reaction was then treated with isopropanol (10 mL) and (S)-carbonic acid 1-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester 4-nitro-phenyl ester (81 mg, 0.20 mmol) and diisopropylethylamine (38 mg, 0.3 mmol). The reaction was stirred at room temperature for 24 hours and then at 60° C. for 4 hours. The volatiles were then removed in vacuo and the reaction was picked up in ethyl acetate and extracted with 1 M HCl. The organics were dried over Na₂SO₄ and the solvent was removed. The residue was purified by silica gel chromatography using a linear gradient of 0-100% ethyl acetate in hexane to afford 64 mg (62%) of material; ¹H NMR (400 MHz, CDCl₃) δ 7.16 (d, J=8.0 Hz, 1H), 6.72 (dd, J=8.4, 2.6 Hz, 1H), 6.66 (ddd, J=8.9, 8.9, 2.7 Hz, 1H), 6.39 (dd, J=8.6, 4.1 Hz, 1H), 5.08 (dd, J=10.3, 3.0 Hz, 1H), 4.44 (m, 1H), 4.03 (m, 2H), 3.70 (m, 2H), 3.44-3.64 (m, 7H), 3.32 (d, J=6.6 Hz, 1H), 3.29 (m, 1H), 3.24 (d, J=8.4 Hz, 1H), 1.55 (m, 8H), 1.25-1.37 (m, 2H), 1.29 (s, 3H), 1.27 (s, 3H), 1.12 (m, 2H), 0.78-0.93 (m, 1H); HPLC-MS calcd. for C₂₈H₄₀FN₃O₅ (M+H⁺) 518.6, found 518.6.

A similar sequence of reactions converted 1-(4-(R)-Azido-tetrahydro-furan-3-(S)-yl)-5-fluoro-3,3-dimethyl-2,3-dihydro-1H-indole to the corresponding [4-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-tetrahydro-furan-3-(R)-yl]-carbamic acid (S)-1-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester: HPLC-MS calcd. for C₂₈H₄₀FN₃O₅ (M+H⁺) 518.6, found 518.6.

Example 8 2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

The title compound was synthesized according to the procedure described in Example 6 as a off-white solid: HPLC-MS calcd. for C₂₆H₃₈FN₃O₃ (M+H⁺) 460.3, found 460.5.

Example 9 3-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

Step A: (R)-2-(Cyclohexylmethyl)succinic acid-1-methyl ester (211 mg, 0.93 mmol) from Acros Organics was treated with (500 μL, 2.87 mmol, 3.1 eq.) of diisopropylethylamine, HATU (360 mg, 1.096 mmol, 1.2 eq.) and (N-(4-Fluoro-phenyl)-ethane-1,2-diamine) (210 mg, 0.93 mmol, 1 eq.). The reagents were dissolved in dry dichloromethane (5 mL). The reaction was allowed to stir for 5 hours and monitored by LC/MS. Volatiles were removed and the reaction directly purified by automated normal-phase chromatography (0-100% ethyl acetate in hexanes gradient). (R)-2-cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-succinamic acid methyl ester was obtained as clear oil (220 mg, 0.60 mmol, 65%).

Step B: (R)-2-cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-succinamic acid methyl ester (220 mg, 0.60 mmol, 1.0 eq.) was dissolved in a mixture of MeOH (4.5 mL) and H₂O (3 mL) and placed in a 0° C. ice bath. Lithium hydroxide (30 mg, 1.25 mmol, 2.1 eq.) was added in one portion and allowed to stir for 8 hours, slowly warming to 23° C. After the reaction was judged complete by LC/MS, methanol was removed by evaporation. Ethyl acetate (75 mL) was added to the resulting solution and was extracted with 1 M HCl (50 mL). The aqueous phase was extracted 2×75 mL of ethyl acetate and the combined organic phases were washed with saturated sodium bicarbonate (50 mL), saturated sodium chloride (50 mL) dried over magnesium sulfate, filtered and evaporated to provide 183 mg of (R)-2-cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-succinamic acid an yellow oil (0.52 mmol, 86%) and was used directly in the following reaction.

Step C: (R)-2-cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-succinamic acid (220 mg, 0.52 mmol) was treated with morpholine (110 μL, 1.26 mmol, 2.4 eq.) and HATU (238 mg, 0.72 mmol, 1.4 eq.). The reagents were dissolved in dry dichloromethane (4 mL) and treated with diisopropylethylamine (315 μL, 1.81 mmol, 3.5 eq.). The reaction was judged to completion by LC/MS, volatiles were evaporated and the reaction was purified by prep-LC/MS and provided 131 mg (0.25 mmol, 40%) of a off-white solid: HPLC-MS calcd. for C₂₃H₃₄FN₃O₃ (M+H⁺) 420.3, found 420.5.

Example 10 [1-(R)-Benzyloxymethyl-2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

The title compound was synthesized according to the procedure outlined in Example 5 starting from 1-(R)-benzyloxymethyl-2(5-fluoro-2,3-dihydro-indol-1-yl)-ethylamine.

¹H-NMR (CD₃OD) δ 7.35(m, 5H), 6.78 (m, 1H), 6.66(m, 1H), 6.42(m, 1H), 5.28(m, 1H), 4.52(m, 2H), 3.99(m, 1H), 3.60(m, 11H), 3.43(m, 1H), 3.14(m, 2H), 2.87 (m, 2H), 1.80 (m, 1H), 1.67(m, 5H), 1.42(m, 2H), 1.18(m, 3H), 0.92(m, 2H). HPLC-MS calcd. for C₃₂H₄₂FN₃O₅ (M+H⁺) 568.31, found 568.6.

Example 11 [2-(5-Fluoro-2,3-dihydro-indol-1-yl)-1-(R)-hydroxymethyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

The title compound of Example 10 (50 mg, 0.088 mmol) was dissolved in a minimum amount of methanol (approx. 1-2 mL) and a catalytic amount of 10% Pd/C was added. Air was purged from the reaction vessel and H₂ gas was introduced via a balloon. The reaction mixture was stirred for several hours under a H₂ atmosphere, after which point the starting material had disappeared by LCMS. The Pd/C was filtered and the crude material was purified by reverse-phase HPLC to afford the title compound (30 mg, 71% yield).

¹H-NMR (CD₃OD) δ 6.78 (m, 1H), 6.71(m, 1H), 6.49(m, 1H), 5.28(m, 1H), 3.88(m, 1H), 3.62(m, 9H), 3.47(m, 3H), 3.12(m, 2H), 2.89 (m, 2H), 1.80 (m, 1H), 1.67(m, 5H), 1.45(m, 2H), 1.19(m, 3H), 0.92(m, 2H). HPLC-MS calcd. for C₂₅H₃₆FN₃O₅ (M+H⁺) 478.26, found 478.5.

Example 12 Morpholine-4-carboxylic Acid 2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylcarbamoyl]-ethyl Ester

The title compound was synthesized according to the procedure outlined in Example 1 starting from 2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylamine.

HPLC-MS calcd. for C₂₅H₃₆FN₃O₄ (M+H⁺) 462.27, found 462.2.

Example 13 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide

The title compound was synthesized according to the procedure described in Example 6 as a off-white solid: HPLC-MS calcd. for C₂₄H₃₄F₃N₃O₄ (M+H⁺) 486.3, found 486.4.

Example 14 2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

The title compound was synthesized according to the procedure described in Example 6 as a off-white solid: HPLC-MS calcd. for C₂₅H₃₆F₃N₃O₄ (M+H⁺) 500.3, found 500.4.

Example 15 2-(R)-Cyclopentylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

The title compound was synthesized according to the procedure described in Example 21 as a light brown solid: HPLC-MS calcd. for C₂₄H₃₄F₃N₃O₄ (M+H⁺) 486.3, found 486.4.

Example 16 2-(R)-Cyclopentylmethyl-3-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

Step A: 4-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-3-(R)-cyclopentylmethyl-4-oxo-butyric acid tert-butyl ester (620 mg, 1.49 mmol, 1.0 eq.) was dissolved in THF (35 mL) and cooled in a 0° C. ice-water bath. Hydrogen peroxide (31 w/w %) (654 μL, 5.97 mmol, 4.0 eq.) and LiOH (72 mg, 2.98 mmol, 2.0 eq.) in water (7.5 mL) was added to the reaction mixture. The reaction stirred at 0° C. and judged to completion by LC/MS. Saturated sodium sulfite (18 mL) and sodium bicarbonate (18 mL) is added to the reaction. THF was evaporated by rotary evaporation and extracted the aqueous layer with CH₂Cl₂ (3×75 mL) to remove the chiral auxiliary. The aqueous layer was acidified at 0° C. with 6M HCl to pH ˜1 and extracted with ethyl acetate (4×75 mL). The combined organic extracts were dried over MgSO₄, filtered and evaporated to provide 281 mg (1.10 mmol, 73%) of 2-(R)-Cyclopentylmethyl-succinic acid 4-tert-butyl ester as clear oil and used directly in Step B. HPLC-MS calcd. for C₁₄H₂₄O₄ (M+Na⁺) 279.3, found 279.3.

Step B: 2-(R)-Cyclopentylmethyl-succinic acid 4-tert-butyl ester (138 mg, 0.54 mmol, 1.0 eq.) was dissolved in dry THF (3 mL) and cooled to −78° C. in an acetone/dry ice bath. Lithium diisopropylamide (2.0M in THF, 600 μL, 1.20 mmol, 2.2 eq.) was added to the reaction and allowed to stir at −78° C. for 1 hour. Methyl iodide (40 μL, 0.65 mmol, 1.2 eq.) was added and the reaction stirred at −78° C. for 2 hours. MeOH (2 mL) was added at −78° C. to quench the reaction. THF and MeOH were removed by rotary evaporation and dissolved in ethyl acetate (30 mL). The organic layer was washed with 1M HCl (20 mL), saturated NaHCO₃ (20 mL), and brine. Dried organic layer over magnesium sulfate, filtered and evaporated to provide 130 mg (0.51, 96%) of a clear oil. The reaction provides a 3:1 mixture of starting material and desired product, 2-(R)-Cyclopentylmethyl-3-(R)-methyl-succinic acid 4-tert-butyl ester. The material is used directly. HPLC-MS calcd. for C₁₅H₂₆O₄ (M+Na⁺) 293.3, found 293.3.

Step C: 3-(R)-Cyclopentylmethyl-2-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-succinamic acid tert-butyl ester was prepared according to example 21. The product was isolated and used directly in the next reaction. HPLC-MS calcd. for C₂₅H₃₇F₃N₂O₄ (M+H⁺) 487.3, found 487.4.

Step D: 3-(R)-Cyclopentylmethyl-2-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-succinamic acid was prepared according to example 21. The product was isolated and used directly in the next reaction. HPLC-MS calcd. for C₂₁H₂₉F₃N₂O₄ 431.2 (M+H⁺), found 431.4.

Step E: The title compound 2-(R)-Cyclopentylmethyl-3-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide was prepared according to example 21. The product was isolated by mass-directed HPLC to provide 12 mg of a white solid after evaporation and lyophilization (0.020 mmol, 3.6% over 3 steps). HPLC-MS calcd. for C₂₅H₃₆F₃N₃O₄ (M+H⁺) 500.3, found 500.5.

Example 17 Morpholine-4-carboxylic Acid {2-benzylsulfanyl-1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide

Step A: 2-(R)-Amino-3-benzylsulfanyl-propionic acid (1.21 g, 5.75 mmol, 1.0 eq.) was suspended in acetonitrile (18 mL) and water (1.5 mL) and treated with Et₃N and allowed to stir at 23° C. for 20 minutes. Added morpholine carbonyl chloride via syringe and allowed to stir for 3 hours and monitored by LC/MS. Upon completion, the reaction was diluted with ethyl acetate (150 mL) and extracted with 1M HCl (50 mL), saturated NaHCO₃ (50 mL) and brine. Dried organic layer over magnesium sulfate, filtered and evaporated to provide 2.80 g of 3-(R)-Benzylsulfanyl-2-[(morpholine-4-carbonyl)-amino]-propionic acid as a light yellow oil that was used directly in the next step. HPLC-MS calcd. for C₁₅H₂₀N₂O₄S (M+H⁺) 325.1, found 325.3.

Step B: 3-Benzylsulfanyl-2-(R)-[(morpholine-4-carbonyl)-amino]-propionic acid (182 mg, 0.56 mmol, 1.2 eq.), N¹-(4-Trifluoromethoxy-phenyl)-(S)-propane-1,2-diamine from Scheme 1a (110 mg, 0.47 mmol, 1.0 eq.), and HATU (185 mg, 0.56 mmol, 1.2 eq.) were dissolved in CH₂Cl₂ (2 mL) at room temperature. DIPEA (245 μL, 1.41 mmol, 3.0 eq.) was added via syringe and the resulting mixture was monitored by LC/MS to completion. After the reaction was complete, the solvent was evaporated and directly purified by mass-directed HPLC and provided 22 mg (0.03 mmol, 5%) of a off-white solid (mono-TFA salt): HPLC-MS calcd. for C₂₅H₃₁F₃N₄O₄S (M+H⁺) 541.2, found 541.3.

Example 18 Morpholine-4-carboxylic Acid {1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-2-phenylmethanesulfonyl-ethyl}-amide

Step A: 3-Benzylsulfanyl-2-(R)-[(morpholine-4-carbonyl)-amino]-propionic acid (780 mg, 2.41 mmol, 1.0 eq.) was dissolved in CH₂Cl₂ and placed in a 0° C. ice-water bath. Added mCPBA (77%) (1.62 g, 7.22 mmol, 3 eq.) in one portion and allowed to stir until completion by LC/MS. Reaction quench with dimethyl sulfide (5 mL) and the solvent was evaporated. Direct purification by mass-directly HPLC provided 2-(R)-[(Morpholine-4-carbonyl)-amino]-3-phenylmethanesulfonyl-propionic acid as a clear oil (73 mg, 0.21 mmol, 9%): HPLC-MS calcd. for C₁₅H₂₀N₂O₆S (M+H⁺) 357.1, found 357.3.

Step B: 2-(R)-[(Morpholine-4-carbonyl)-amino]-3-phenylmethanesulfonyl-propionic acid (73 mg, 0.21 mmol, 1.0 eq.), N¹-(4-Trifluoromethoxy-phenyl)-(S)-propane-1,2-diamine from Scheme 1a (48 mg, 0.21 mmol, 1.0 eq.), EDC (59 mg, 0.31 mmol, 1.5 eq.), and HOBT (38 mg, 0.25 mmol, 1.2 eq.) were dissolved in CH₂Cl₂ at room temperature. N-methylmorpholine (225 μL, 2.05 mmol, 10 eq.) was added via syringe and the reaction was monitored to completion by LC/MS. The solvent was evaporated and the reaction was purified by mass-directed HPLC and provided 44 mg (0.06 mmol, 31%) of a off-white solid as a mono-TFA salt: HPLC-MS calcd. for C₂₅H₃₁F₃N₄O₆S (M+H⁺) 573.2, found 573.3.

Example 19 (R)-2-Cyclohexylmethyl-N-[2-(4-methoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

C₂₄H₃₇N₃O₄; HPLC-MS: 432.5 (M+H⁺).

Example 20 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide

Compound was synthesized in a similar fashion to Example 6 using an appropriate diamine. HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₄ (M+H⁺) 528.3, found 528.6.

Example 21 2-(R)-(2-Cyclohexyl-ethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

Step A: 4-Cyclohexyl-butyric acid (3.4 g, 20.0 mmol, 1.0 eq.) was dissolved in dry CH₂Cl₂ (10 mL) and cooled to 0° C. in an ice water bath. A few drops of DMF was added (˜100 μL) followed by slow addition of thionyl chloride (2.07 mL, 20.0 mmol, 1 eq.) via syringe. The reaction mixture was warmed to room temperature and stirred for 2 hours. Solvent was evaporated and the resulting 4-cyclohexyl-butyryl chloride used directly in Step B without further purification.

Step B: Performed as described in Evans, D. A., et al. Tetrahedron 1988, 44, 5525 using 4-cyclohexyl-butyryl chloride. (S)-4-Benzyl-3-(4-cyclohexyl-butyryl)-oxazolidin-2-one (5.65 g, 17.15 mmol, 88%) was isolated as a white solid. HPLC-MS calcd. for C₂₀H₂₇NO₃ (M+Na⁺) 352.3, found 352.1.

Step C: Performed as described in Evans, D. A., et al. J. Org. Chem. 1999, 64, 6411 using (S)-4-benzyl-3-(4-cyclohexyl-butyryl)-oxazolidin-2-one. 3-(R)-(4-(S)-Benzyl-2-oxo-oxazolidine-3-carbonyl)-5-cyclohexyl-pentanoic acid tert-butyl ester (2.5 g, 5.63 mmol, 84%) was isolated as a clear oil and >20:1 mixture of diastereomers: ¹H NMR (CDCl₃, 400 MHz) δ 0.66-0.78 (m, 2H), 0.96-1.14 (m, 6H), 1.30 (s, 9H), 1.32-1.56 (m, 7H), 2.34 (dd, 1H, J=16.8, 4.0 Hz), 2.61 (dd, 1H, J=12.4, 6.0 Hz), 2.66 (dd, 1H, J=16.8, 10.4 Hz), 3.21 (dd, 1H, J=13.6, 3.2 Hz), 3.96-3.21 (m, 3H), 4.55 (m, 1H), 7.13-7.23 (m, 5H).

Step D: 3-(R)-(4-(S)-Benzyl-2-oxo-oxazolidine-3-carbonyl)-5-cyclohexyl-pentanoic acid tert-butyl ester (2.01 g, 4.53 mmol, 1.0 eq) was treated with a 45:50:5 CH₂Cl₂:TFA:H₂O solution. The reaction was monitored by LC/MS and complete after 1 hour. The solution was evaporated and provided a quantitative yield of 3-(R)-(4-(S)-Benzyl-2-oxo-oxazolidine-3-carbonyl)-5-cyclohexyl-pentanoic acid as a yellow oil. ¹H NMR (CDCl₃, 400 MHz) δ 0.80-0.91 (m, 2H), 1.09-1.27 (m, 6H), 1.44-1.53 (m, 1H), 1.61-1.73 (m, 6H), 2.12 (s, 2H), 2.35 (dd, 1H, J=17.2, 4.0 Hz), 2.49 (dd, 1H, J=13.6, 9.6 Hz), 2.71 (dd, 1H, J=17.6, 10.8 Hz), 3.03 (dd, 1H, J=13.6, 3.2 Hz), 3.87-3.97 (m, 3H), 4.43 (m, 1H), 6.90-7.10 (m, 5H).

Step E: 3-(R)-(4-(S)-Benzyl-2-oxo-oxazolidine-3-carbonyl)-5-cyclohexyl-pentanoic acid (1.76 g, 4.53 mmol, 1.0 eq.) dissolved in DMF (10 mL) and treated with HATU (1.3 g, 5.0 mmol, 1.1 eq.), morpholine (680 μL, 7.77 mmol, 1.7 eq.) and DIPEA (870 μL, 5.0 mmol, 1.1 eq.). Alternatively an additional equivalent of morpholine can be used in the reaction and CH₂Cl₂ can be used as the reaction solvent. The reaction is monitored by LC/MS. The reaction mixture is diluted with ethyl acetate (50 mL) and extracted with 0.5 M HCl (2×10 mL), saturated NaHCO₃, and brine. The organic layer is dried over MgSO₄, filtered and evaporated. The crude mixture is used in Step F directly or purified by normal-phase silica chromatography in a 20-50% ethyl acetate in hexanes gradient to provide 1-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-2-(R)-(2-cyclohexyl-ethyl)-4-morpholin-4-yl-butane-1,4-dione as a white solid (1.91 g, 4.18 mmol, 92%): ¹H NMR (CDCl₃, 400 MHz) δ 0.80-0.91 (m, 2H), 1.09-1.27 (m, 6H), 1.44-1.53 (m, 1H), 1.61-1.73 (m, 6H), 2.12 (s, 2H), 2.35 (dd, 1H, J=17.2, 4.0 Hz), 2.49 (dd, 1H, J=13.6, 9.6 Hz), 2.71 (dd, 1H, J=17.6, 10.8 Hz), 3.03 (dd, 1H, J=13.6, 3.2 Hz), 3.48-3.72 (m, 8H), 3.87-3.97 (m, 3H), 4.43 (m, 1H), 6.90-7.10 (m, 5H), 8.02 (s, 1H).

Step F: The chiral auxiliary was removed in an identical manner to Step A of Example 16. 2-(R)-(2-Cyclohexyl-ethyl)-4-morpholin-4-yl-4-oxo-butyric acid was isolated as a white solid (1.0 g, 3.36 mmol, 80%). HPLC-MS calcd. for C₁₆H₂₇NO₄ (M+H⁺) 298.2, found 298.1.

Step G: 2-(R)-(2-Cyclohexyl-ethyl)-4-morpholin-4-yl-4-oxo-butyric acid (98 mg, 0.33 mmol, 1.0 eq.) dissolved in DMF (2 mL) and treated with HATU (137 mg, 0.36 mmol, 1.1 eq.), (S)—N¹-(4-Trifluoromethoxy-phenyl)-propane-1,2-diamine (85 mg, 0.36 mmol, 1.1 eq.) and DIPEA (63 μL, 0.36 mmol, 1.1 eq.). Alternatively CH₂Cl₂ can be used as the reaction solvent based on starting material solubility. The reaction is monitored by LC/MS. The reaction mixture is diluted with ethyl acetate (20 mL) and extracted with 0.5 M HCl (2×10 mL), saturated NaHCO₃, and brine. The organic layer is dried over MgSO₄, filtered and evaporated. Alternatively, the crude reaction can be directly purified by mass-directed HPLC. Mass-directed HPLC provides the title compound as a white solid after evaporation and lyophilization: ¹H NMR (CD₃OD, 400 MHz) δ 0.74-0.82 (m, 2H), 1.08-1.65 (m, 13H), 1.21(d, J=6.8 Hz, 3H), 2.38(dd, J=4.8, 15.6 Hz, 1H), 2.65(m, 1H), 2.74(dd, J=9.6, 15.6 Hz, 1H), 3.10(m, 2H), 3.54-3.66(m, 8H), 4.11 (m, 1H), 6.45(m, 2H), 6.97(m, 2H). HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.2.

Example 22 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

The compound was synthesized in an analogous fashion to Example 6. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.6.

Example 23 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

The compound was synthesized in an analogous fashion to Example 6. HPLC-MS calcd. for C₃₂H₄₂F₃N₃O₄ (M+H⁺) 590.3, found 590.6.

Example 24 2-(R)-Cyclohexylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

The compound was synthesized in an analogous fashion to Example 6. HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₄ (M+H⁺) 528.3, found 528.6.

Example 25 5,5-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic Acid [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide

5,5-Dimethyl-hex-2-enoic acid was prepared using a modified procedure from Chatterjee, A. K. et al. J. Am. Chem. Soc. 2003, 125(37), 11360-11370. 4,4-Dimethyl-1-pentene (5.0 mL, 34.77 mmol, 1.5 eq.) and acrylic acid (1.54 mL, 22.51 mmol, 1.0 eq.) were added to a solution of ruthenium catalyst ([1,3-bis(2,4,6-trimethylphenyl)-2-imidazolidinylidene]dichloro[[2-(1-methylethoxy-)phenyl]methylene-]ruthenium, 282 mg, 0.45 mmol, 2 mol %) in CH₂Cl₂ and heated to reflux for 12 hours under nitrogen atmosphere. The resulting brown solution was diluted with CH₂Cl₂ and washed with 1M NaOH (3×15 mL). The organic phase was acidified with 4 M HCl (20 mL) and extracted with EtOAc (3×25 mL). The combined organics were washed with brine, dried over MgSO₄, filtered and evaporated to provide intermediate 5,5-Dimethyl-hex-2-enoic acid as a light brown oil (3.16 g, 22.21 mmol, 99%): ¹H NMR (CDCl₃, 400 MHz) δ 0.94 (s, 9H), 2.11 (d, 2H, J=8.0 Hz), 5.82 (d, 1H, J=16.0 Hz), 7.12 (dt, 1H, J=16.0, 8.0 Hz).

5,5-Dimethyl-hex-2-enoic acid was reduced to 5,5-dimethyl-hexanoic acid by the procedure described in example 26, step B.

5,5-Dimethyl-hexanoic acid was converted to the title compounds using the procedures that described in Example 21.

¹H NMR (CD₃OD, 400 MHz) δ 0.80 (s, 9H), 1.18(m, 2H), 1.21(d, J=6.8 Hz, 3H), 1.43-1.52(m, 2H), 2.40(dd, J=4.8, 16 Hz, 1H), 2.61(m, 1H), 2.76(dd, J=9.6, 15.6 Hz, 1H), 3.11(m, 2H), 3.52-3.65(m, 8H), 4.10(m, 1H), 6.64(m, 2H), 6.98(m, 2H). HPLC-MS calcd. for C₂₄H₃₆F₃N₃O₄ (M+H⁺) 488.3, found 488.2.

Example 26 4,4-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-pentanoic Acid [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide

Step A: Methyl diethylphosphonoacetate (13.35 mL, 73.6 mmol, 1.05 eq.) was dissolved in THF (30 mL) and cooled to −78° C. in an acetone-dry ice bath. n-BuLi (1.6M in hexanes) (45.8 mL, 73.2 mmol, 1.05 eq.) was added slowly over 20 min. Trimethylacetaldehyde (6.0 g, 69.6 mmol, 1.0 eq.), was added to the reaction and allowed to stir at −78° C. for 20 minutes and warmed to room temperature and stirred overnight. Water (30 mL) was added to quench and extracted with ethyl ether (3×100 mL). The combined organics were washed with brine and dried over MgSO₄, filtered and evaporated to provide 4,4-Dimethyl-pent-2-enoic acid methyl ester, a colorless liquid (8.52 g, 60.0 mmol, 86%) and was used directly in the next reaction. ¹H NMR (CDCl₃, 400 MHz) δ 0.89 (s, 9H), 3.64 (s, 3H), 5.64 (d, 1H, J=16.0 Hz), 6.88 (d, 1H, J=16.0 Hz).

Step B: 4,4-Dimethyl-pent-2-enoic acid methyl ester (8.52 g, 60.0 mmol) was dissolved in MeOH (30 mL) and ethyl acetate (30 mL). Palladium on carbon (10 wt %) (˜50 mg) was added to the reaction and was placed under a H₂ balloon (1 atm). The reaction was allowed to stir for 12 hours, flushed with nitrogen and filtered over a pad of celite. Evaporated to provide 4,4-dimethyl-pentanoic acid methyl ester (7.0 g, 48.5 mmol, 81%) as a colorless oil: ¹H NMR (CDCl₃, 400 MHz) δ 0.89 (s, 9H), 1.53-1.57 (m, 2H), 2.26-2.30 (m, 2H), 3.67 (s, 3H).

Step C: 4,4-Dimethyl-pentanoic acid methyl ester (7.0 g, 48.5 mmol, 1.0 eq.) was treated with a solution of NaOH (4.0 g, 100 mmol, 2.1 eq.) in water (5 mL). The homogeneous solution was allowed to stir for 4-5 hours. The reaction was diluted with CH₂Cl₂ and the aqueous layer was extracted with CH₂Cl₂ (50 mL×3). The aqueous layer was acidified with 6M HCl to pH ˜1.5 and extracted with ethyl acetate (50 mL×3). The combined ethyl acetate fractions were dried over MgSO₄, filtered and evaporated to provide 4,4-dimethyl-pentanoic acid (5.35 g, 41.1 mmol, 85%) as a colorless oil: ¹H NMR (CDCl₃, 400 MHz) δ 0.89 (s, 9H), 1.53-1.57 (m, 2H), 2.26-2.30 (m, 2H).

4,4-dimethyl-pentanoic acid was converted to the title compounds using the procedures that described in Example 21.

¹H NMR (CD₃OD, 400 MHz) δ 0.90 (s, 9H), 1.18(d, J=6.8 Hz 3H), 1.20-1.29(m, 2H), 1.77(dd, J=9.2, 14 Hz, 1H), 2.39(dd, J=6.4, 15.6 Hz, 1H), 2.61(dd, J=8.4, 15.6 Hz, 1H), 2.82(m, 1H), 2.99-3.05(m, 1H), 3.21(dd, J=6.8, 12.8 Hz, 1H), 3.50-3.65(m, 8H), 4.04(m, 1H), 6.63-6.66(m, 2H), 6.96-6.99(m, 2H). HPLC-MS calcd. for C₂₃H₃₄F₃N₃O₄ (M+H⁺) 474.3, found 474.2.

Example 27 2-(R)-Cyclopentylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.5.

Example 28 2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 6. HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₆S (M+H⁺) 592.3, found 592.5.

Example 29 2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(R)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound was isolated as a minor diastereomers from the synthesis of Example 28. HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₆S (M+H⁺) 592.3, found 592.5.

Example 30 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-5-phenyl-pentanoic Acid {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide

Compound was synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₉H₃₈F₃N₃O₄ (M+H⁺) 550.3, found 550.5.

Example 31 2-(R)-Cyclopentylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 0.96 (d, J=6.8 Hz, 3H), 0.98(d, J=6.8 Hz, 3H), 1.03-1.93(m, 12H), 2.39(dd, J=4.4, 15.2 Hz, 1H), 2.70-2.76(m, 1H), 3.11(dd, J=8.4, 12.8 Hz, 1H), 3.26(dd, J=4.0, 12.4 Hz, 1H), 3.52-3.66(m, 8H), 3.87(m, 1H), 6.59-6.63(m, 2H), 6.98-7.00(m, 2H). HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.2.

Example 32 N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-phenethyl-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 0.97 (d, J=6.8 Hz, 3H), 1.0(d, J=6.8 Hz, 3H), 1.70-1.94(m, 3H), 2.43(dd, J=3.2, 14.8 Hz, 1H), 2.55-2.60(m, 2H), 2.80(m, 1H), 3.14(dd, J=9.2, 13.2 Hz, 1H), 3.28(m, 1H), 3.51-3.63(m, 8H), 3.93(m, 1H), 4.61(bs, 1H), 6.60(m, 2H0, 6.95(m, 2H), 7.05-7.18(m, 5H). HPLC-MS calcd. for C₂₈H₃₆F₃N₃O₄ (M+H⁺) 536.3, found 536.5.

Example 33 2-(R)-(2-Cyclopentyl-ethyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 25 using allylcyclopentene.

¹H NMR (CD₃OD, 400 MHz) δ 0.97 (d, J=6.8 Hz, 3H), 1.0(d, J=6.8 Hz, 3H), 1.30-1.69(m, 12H), 1.90(m, 1H), 2.38(m, 1H), 2.75-2.82(m, 2H), 3.11(dd, J=8.4, 12.4 Hz, 1H), 3.26(dd, J=4.4, 12.4 Hz, 1H), 3.54-3.67(m, 8H), 3.89(m, 1H), 6.59-6.65(m, 2H), 6.97-7.00(m, 2H). HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₄ (M+H⁺) 528.3, found 528.5.

Example 34 N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₆H₃₂F₃N₃O₄ (M+H⁺) 508.2, found 508.4.

Example 35 2-(S)-Cyclohexyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.4.

Example 36 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

Compound is synthesized in accordance with Example 25. ¹H NMR (CD₃OD, 400 MHz) δ 1.13(t, J=6.8 Hz, 3H), 1.14-1.84(m, 14H), 2.55(dd, J=3.6, 14.8 Hz, 1H), 2.87-2.95(m, 2H), 3.31(d, J=6.8 Hz, 3.69-3.81(m, 8H), 4.05(m, 1H), 6.82-6.85(m, 2H), 7.15-7.17(m, 2H. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₄ (M+H⁺) 514.3, found 514.3.

Example 37 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

Compound is synthesized in accordance with Example 25. ¹H NMR (CD₃OD, 400 MHz) δ 1.15(m, 2H), 1.47-2.10(m, 14H), 5.57(m, 1H), 2.70-2.99(m, 3H), 3.12-3.35(m, 2H), 3.69-3.84(m, 8H), 4.20(m, 1H), 6.80-6.82(m, 2H), 7.14-7.16(m, 2H), 7.31-7.40(m, 5H). HPLC-MS calcd. for C₃₂H₄₂F₃N₃O₄ (M+H⁺) 590.3, found 590.3.

Example 38 4-Morpholin-4-yl-4-oxo-2-(R)-phenethyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 0.98(t, J=7.2 Hz, 3H), 1.45-1.90(m, 4H), 2.40-2.50(m, 1H), 2.58-2.62(m, 2H), 2.80-2.82(m, 2H), 3.19(d, J=6.8 Hz, 2H), 3.51-3.65(m, 8H), 3.95(m, 1H), 6.68-6.70(m, 2H), 6.98-6.99(m, 2H), 7.00-7.21(m, 5H). HPLC-MS calcd. for C₂₇H₃₄F₃N₃O₄ (M+H⁺) 522.3, found 522.2.

Example 39 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 0.81(t, J=7.2 Hz, 3H), 1.20-1.43(m, 4H), 2.52(dd, J=4.8, 16 Hz, 1H), 2.85-2.95(m, 2H), 3.11(dd, J=10, 16 Hz, 1H), 3.38-3.50(m, 8H), 3.86(m, 1H), 3.92(dd, J=4.8, 10.4 Hz, 1H), 6.34-6.36(m, 2H), 6.78-6.80(m, 2H), 7.15-7.26(m, 5H). HPLC-MS calcd. for C₂₆H₃₂F₃N₃O₄ (M+H⁺) 508.3, found 508.2.

Example 40 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 1.76-1.90(m, 2H), 2.58-2.65(m, 2H), 2.72-2.77(m, 1H), 3.00-3.10(m, 2H), 3.24-3.30(m, 1), 3.49-3.66(m, 8H), 3.97(m, 1H), 4.09(dd, J=4.8, 10.4 Hz, 1H), 5.43-6.46(m, 2H), 6.87-6.89(m, 2H), 7.18-7.40(m, 10H). HPLC-MS calcd. for C₃₁H₃₄F₃N₃O₄ (M+H⁺) 570.3, found 570.2.

Example 41 2-(S)-(4-Fluoro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₆H₃₁F₄N₃O₄ (M+H⁺) 526.2, found 526.5.

Example 42 2-(S)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₆H₃₁ClF₃N₃O₄ (M+H⁺) 542.2, found 542.5.

Example 43 2-(R)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is isolated as a minor side product from Example 42 synthesis. HPLC-MS calcd. for C₂₆H₃₁ClF₃N₃O₄ (M+H⁺) 542.2, found 542.5.

Example 44 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-[1-(s)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₉H₃₀F₃N₃O₄ (M+H⁺) 542.2, found 542.5.

Example 45 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide

Compound is synthesized in accordance with Example 6. HPLC-MS calcd. for C₃₀H₃₈F₃N₃O₄ (M+H⁺) 562.3, found 562.5.

Example 46 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide

Compound is synthesized in accordance with Example 25. ¹H NMR (CD₃OD, 400 MHz) δ 1.15-1.64(m, 2H), 1.42-1.85(m, 12H), 2.44(dd, J=3.6, 10.8 Hz, 1H), 2.83-2.92(m, 2H), 3.55-3.65(m 9H), 5.28(t, J=7.2 Hz, 1H), 6.77-6.80(m, 2H), 7.12-7.14(m, 2H), 7.39-7.53(m, 5H). HPLC-MS calcd. for C₃₀H₃₈F₃N₃O₄ (M+H⁺) 562.3, found 562.5.

Example 47 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-pent-4-enoic Acid {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 0.97(d, J=6.8 Hz, 3H), 0.98(d, J=6.8 Hz, 3H), 1.88-1.93(m, 1H), 2.15-2.32(m, 2H), 2.40(dd, J=4, 16 Hz, 1H), 2.73-2.82(m, 1H), 2.85-2.90(m, 1H), 31.0-3.13(m, 1H), 3.24-3.31(m, 1H), 3.51-3.67(m, 8H), 3.90(m, 1H), 4.97-5.08(m, 2H), 5.74-5.81(m, 1H), 6.61-6.63(m, 2H), 6.98-7.01(m, 2H). HPLC-MS calcd. for C₂₃H₃₂F₃N₃O₄ (M+H⁺) 472.3, found 472.2.

Example 48 2-(S)-(4-Chloro-phenyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide

Compound is synthesized in accordance with Example 21. ¹H NMR (CD₃OD, 400 MHz) δ 2.63(dd, J=4.4, 15.6 Hz, 1H), 3.26(dd, J=10.0, 16 Hz, 1H), 3.42-3.69(m, 9H), 4.14-4.21(m, 1H), 5.13(dd, J=6, 8.8 Hz, 1H), 6.50-6.53(m, 2H), 6.97-7.00(m, 2H), 7.27-7.52(m, 10H). HPLC-MS calcd. for C₂₉H₂₉ClF₃N₃O₄ (M+H⁺) 576.2, found 576.1.

Example 49 (R)-5,5-Dimethyl-2-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic Acid [2-(5-methyl-isoxazol-3-ylamino)-ethyl]-amide

C₂₀H₃₄N₄O₄; HPLC-MS: 395.5 (M+H⁺).

Example 50 2-(R)-Cyclohexylmethyl-4-(cis-2,6-dimethyl-morpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide

Compound is synthesized in accordance with Example 6, using cis-2,6-dimethylmorpholine. ¹H NMR (CD₃OD, 400 MHz) δ 0.79-0.85(m, 2H), 1.06-1.21(m, 14H), 1.42-1.82(m, 6H), 2.22-2.40(m, 2H), 2.61-2.76(m, 3H), 3.01-3.13(m, 2H), 3.36-3.55(m, 2H), 3.77-3.81(m, 1H), 4.05(m, 1H), 4.26-4.29(m, 1H), 6.62-6.65(m, 2H), 6.95-6.97(m, 2H). HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₄ (M+H⁺) 528.3, found 528.6.

Example 51 2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-4-thiomorpholin-4-yl-butyramide

Compound is synthesized in accordance with Example 6, using thiomorpholine. ¹H NMR (CD₃OD, 400 MHz) δ 0.81-0.85(m, 2H), 1.08-1.23(m, 5H), 1.19(d, J=6.8 Hz, 3H), 1.42-1.83(m, 6H), 2.35(dd, J=4.4, 15.6 Hz, 1H), 2.50-2.76(m, 6H), 3.09(dd, J=5.6, 12.4 Hz, 1H), 3.20(dd, J=7.6, 12.8 Hz, 1H), 3.66-3.85(m, 4H), 4.01-4.07(m, 1H), 6.73-6.75(m, 2H), 7.01-7.03(m, 2H). HPLC-MS calcd. for C₂₅H₃₆F₃N₃O₄ (M+H⁺) 516.2, found 516.5.

Example 52 4-(4-Acetyl-piperazin-1-yl)-2-(R)-cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide

Compound is synthesized in accordance with Example 6, using l-acetyl-piperazine. ¹H NMR (CD₃OD, 400 MHz) δ 00.74-0.78(m, 2H), 0.98-1.16(m, 5H), 1.11(d, J=6.8 Hz, 3H), 1.36-1.76(m, 6H), 2.01(s, 3H), 2.30-2.35(m, 1H), 2.59-2.72(m, 2H), 2.98(dd, J=6.0, 12.8 Hz, 1H), 3.07-3.09(m, 2H), 3.35-3.54(m, 8H), 3.96-4.01(m, 1H), 6.59-6.61(m, 2H), 6.90-6.92(m, 2H). HPLC-MS calcd. for C₂₇H₃₉F₃N₃O₄ (M+H⁺) 541.3, found 541.6.

Example 53 2-(S)-(4-Methoxy-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₇H₃₄F₃N₃O₅ (M+H⁺) 538.3, found 538.5.

Example 54 2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

HPLC-MS for C₂₈H₄₂FN₃O₃ (M+1)=488.4.

Example 55 N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₇H₃₁F₆N₃O₄ (M+H⁺) 576.2, found 576.5.

Example 56 N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-(4-trifluoromethyl-phenyl)-butyramide

Compound is synthesized as a minor side product from the synthesis of Example 55. HPLC-MS calcd. for C₂₇H₃₁F₆N₃O₄ (M+H⁺) 576.2, found 576.5.

Example 57 N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-p-tolyl-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₇H₃₄F₃N₃O₄ (M+H⁺) 522.3, found 522.5.

Example 58 2-(R)-Cyclohexylmethyl-4-(1,1-dioxo-thiomorpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide

The compound was synthesized in a similar fashion to Example 6. An OXONE oxidation was employed title compound of Example 51 to furnish the sulfone. A procedure was used as described in McCarthy, J. R. et al. Org. Synth., CV9, 446. ¹H NMR (CD₃OD, 400 MHz) δ 0.73-0.79(m, 2H), 1.00-1.19(m, 7H), 1.10(d, J=6.8 Hz), 1.34-1.75(m, 6H), 2.34-2.37(m, 1H), 2.64-2.71(m, 2H), 2.94-3.08(m, 7H), 3.79-4.01(m, 5H), 6.53-6.56(m, 2H), 6.87-6.89(m, 2H). HPLC-MS calcd. for C₂₅H₃₆F₃N₃O₅S (M+H⁺) 548.2, found 548.2.

Example 59 2-(R)-(3-Ethyl-3-hydroxy-cyclohexylmethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

¹H NMR (CD₃OD, 400 MHz) δ 0.70(t, J=7.6 Hz, 3H), 0.95-1.57(m, 13H), 1.12(d, J=6.8 Hz, 3H), 2.30(dd, J=4.4, 15.6 Hz, 1H), 2.58-2.64(m, 1H), 2.72(m, 1H), 3.00(dd, J=5.2, 12.4 Hz, 1H), 3.07(dd, J=7.6, 12.4 Hz, 1H), 3.38-3.57(m, 8H), 4.02-4.04(m, 1H), 6.58-6.61(m, 2H), 6.91-6.93(m, 2H). HPLC-MS calcd. for C₂₇H₄₀F₃N₃O₅ (M+H⁺) 544.3, found 544.2.

Example 60 N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₅H₂₇F₆N₃O₄ (M+H⁺) 548.2, found 548.4.

Example 61 N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₄H₂₈F₃N₃O₄ (M+H⁺) 480.2, found 480.4.

Example 62 2-(S)-(4-Fluoro-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₄H₂₇F₄N₃O₄ (M+H⁺) 498.2, found 498.4.

Example 63 2-(S)-(4-Methoxy-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₂₅H₃₀F₃N₃O₅ (M+H⁺) 510.2, found 510.4.

Example 64 2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(6-methoxy-pyridin-3-ylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Step A: 2-(S)-(4-Fluoro-phenyl)-4-morpholin-4-yl-4-oxo-butyric acid (2.00 g, 7.11 mmol, 1.0 eq.), 2-(S)-Amino-3-methyl-butan-1-ol (1.59 g, 7.33 mmol, 1.03 eq.) and HATU (2.41 g, 7.33 mmol, 1.03 mmol) were dissolved in CH₂Cl₂ and stirred at room temperature. Diisopropylethylamine (3.9 mL, 21.98 mmol, 3.1 eq.) was added via syringe and the reaction is monitored by LC/MS. Upon completion, there reaction was evaporated and is diluted with ethyl acetate (100 mL) and extracted with 0.5M HCl (2×30 mL), saturated NaHCO₃, and brine. The organic layer is dried over MgSO₄, filtered and evaporated. Column chromatography (0-100%) EtOAc in hexane gradient provided 2-(S)-(4-Fluoro-phenyl)-N-(1-(S)-hydroxymethyl-2-methyl-propyl)-4-morpholin-4-yl-4-oxo-butyramide as a clear oil (1.06 g, 2.89 mmol, 41%). HPLC-MS calcd. for C₁₉H₂₇FN₂O₄ (M+H⁺) 367.2, found 367.4; TLC (1:1 hexane/EtOAc) R_(f)=0.15.

Step B: 2-(S)-(4-Fluoro-phenyl)-N-(1-(S)-hydroxymethyl-2-methyl-propyl)-4-morpholin-4-yl-4-oxo-butyramide (2.31 g, 6.30 mmol, 1.0 eq.) was dissolved in CH₂Cl₂ (32 mL) and stirred at room temperature. Dess-Martin Periodinane (2.67 g, 6.30 mmol, 1.0 eq.) was added in one portion and the reaction is monitored by LC/MS and TLC. Upon completion, the reaction is diluted with EtOAc (200 mL) and extracted with 1M Na₂S₂O₃ (100 mL), NaHCO₃ (100 mL) and brine. The organic phase is dried over MgSO₄, filtered and evaporated to provide 2-(S)-(4-Fluoro-phenyl)-N-(1-(S)-formyl-2-methyl-propyl)-4-morpholin-4-yl-4-oxo-butyramide (1.41 g, 3.89 mmol, 62%) and used directly in the next step. HPLC-MS calcd. for C₁₉H₂₅FN₂O₄ (M+H⁺) 365.2, found 365.4.

Step C: 2-(S)-(4-Fluoro-phenyl)-N-(1-(S)-formyl-2-methyl-propyl)-4-morpholin-4-yl-4-oxo-butyramide (350 mg, 0.96 mmol, 1.0 eq.) and 5-Amino-2-methoxy-pyridine (240 μL, 1.92 mmol, 2.0 eq.) dissolved in MeOH (2.5 mL) and acetic acid (106 mL, 1.92 mmol, 2.0 eq.) added via syringe at room temperature. Sodium cyanoborohydride (121 mg, 1.92 mmol, 2.0 eq.) added in portion and the reaction monitored the reaction by LC/MS. Upon completion, solvents were evaporated and dissolved in EtOAc (100 mL) washed with brine, dried over MgSO₄, filtered and evaporated. Purification by preparative mass-directed HPLC provided the title compound as a white solid after evaporation and lyophilizationn(2.6 mg, 5.6 □mol, 0.6%). HPLC-MS calcd. for C₂₅H₃₃FN₄O₄ (M+H⁺) 473.3, found 473.5.

Example 65 N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(R)-spiro[2.5]oct-6-ylmethyl-butyramide.

Step A: 4-Methylene-cyclohexanecarboxylic acid ethyl ester (9.0 g, 65.1 mmol, 91%) was synthesized, using a procedure from Della, E. W. et al. J. Org. Chem. 1993, 58, 2110, from commercially available 4-oxo-cyclohexanecarboxylic acid ethyl ester and used directly in the next reaction. ¹H NMR (CHCl₃, 400 MHz) δ 1.23-1.27 (m, 3H), 1.55-1.62 (m, 2H), 1.97-2.09 (m, 4H), 2.31-2.46 (m, 3H), 4.09-4.19 (m, 2H), 4.64 (s, 2H).

Step B: The reduction reaction of 4-methylene-cyclohexanecarboxylic acid ethyl ester with LAH is as described in the above reference. The product was purified by distillation to provide (4-methylene-cyclohexyl)-methanol as clear oil (3.67 g, 29.09 mmol, 50% over Step A and B).

Step C: (4-methylene-cyclohexyl)-methanol was cyclopropanated, as described in Boehm, M. F. et al. J. Med. Chem. 1995, 38, 3146, to provide Spiro[2.5]oct-6-yl-methanol (340 mg, 2.42 mmol, 75%) as a pale yellow oil. ¹H NMR (CHCl₃, 400 MHz) δ 0.17-0.27 (m, 4H), 0.88-0.91 (m, 2H), 1.09-1.25 (m, 2H), 1.52-1.54 (m, 2H), 1.69-1.75 (m, 3H), 3.49-3.51 (m, 2H).

Step D: Spiro[2.5]oct-6-yl-methanol (2.57 g, 18.3 mmol, 1.0 eq.) was dissolved in CH₂Cl₂ (30 mL) and placed in a ice-water bath at 0° C. Pyridinium dichromate (PCC) (7.9 g, 36.6 mmol, 2.0 eq.) was added and the reaction warmed to room temperature for 4 hours with stirring. The crude reaction was filteredd through a Celite pad and washed with CH₂Cl₂ and evaporated. The crude material was dissolved in diethyl ether and filtered through Celite, washing with diethyl ether several times. Evaporated solvent to provide Spiro[2.5]octane-6-carbaldehyde as a brown oil (2.98 g, quantitative) that was used directly. ¹H NMR (CHCl₃, 400 MHz) δ 0.17-0.27 (m, 4H), 0.88-0.91 (m, 2H), 1.09-1.25 (m, 2H), 1.52-1.54 (m, 2H), 1.69-1.75 (m, 3H), 9.44 (d, 1H, J=1.2 Hz).

4-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-4-oxo-3-(R)-spiro[2.5]oct-6-ylmethyl-butyric acid tert-butyl ester was prepared according to the procedures described in Example 26. A modified procedure was used for the t-Butyl ester deprotection from Trzeciak, A. et al. Synthesis 1996, 1443. 4-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-4-oxo-3-(R)-spiro[2.5]oct-6-ylmethyl-butyric acid tert-butyl ester (40 mg, 0.09 mmol, 1.0 eq.) was dissolved in dry dioxane (5 mL) under nitrogen followed by addition of Et₃N (200 μL, 1.43 mmol, 16 eq.) and TMS-OTf (200 μL, 1.11 mmol, 12.3 eq.) via syringe. The reaction mixture stirred at room temperature overnight and then heated to 65° C. for 1.5 hours. Upon completion, 2 mL of water was added and volatiles were evaporated. The product was diluted with ethyl ether and extracted with water, and brine. The organic layer was dried over MgSO₄, filtered and evaporated to provide 4-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-4-oxo-3-(R)-spiro[2.5]oct-6-ylmethyl-butyric acid (50 mg, quantitative) as white solid and used directly in the next step. HPLC-MS calcd. for C₂₃H₂₉NO₅ (M+Na⁺) 422.2, found 422.1. 4-(4-(S)-Benzyl-2-oxo-oxazolidin-3-yl)-4-oxo-3-(R)-spiro[2.5]oct-6-ylmethyl-butyric acid was converted to the title compound according to the procedures described in example 21.

N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(R)-spiro[2,5]oct-6-ylmethyl-butyramide: ¹H NMR (CD₃OD, 400 MHz) δ 0.05-0.10(m, 4H), 0.63-1.63(m, 10H), 1.05(d, J=4.0 Hz, 3H), 2.23(dd, J=4.8, 15.6 Hz, 1H), 2.55(dd, J=9.6, 15.6 Hz, 1H), 2.65(m, 1H), 2.92(dd, J=6.0, 12.8 Hz, 1H), 3.02(dd, J=7.2, 12.4 Hz, 1H), 3.32-3.51(m, 8H), 3.92-3.96(m, 1H), 6.50-6.53(m, 2H), 6.81-6.85(m, 2H). HPLC-MS calcd. for C₂₇H₃₈F₃N₃O₄ (M+H⁺) 526.3, found 526.5.

Example 66 N-[2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

HPLC-MS for C₂₈H₃₃F₄N₃O₃ (M+1)=536.4.

Example 67 2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(3-methanesulfonyl-phenylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 64. HPLC-MS calcd. for C₂₆H₃₄FN₃O₅S (M+H⁺) 520.2, found 520.5.

Example 68 N-[1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

Compound is synthesized in accordance with Example 21. HPLC-MS calcd. for C₃₀H₃₇F₄N₃O₃ (M+H⁺) 564.3, found 564.5.

Example 69 N-[1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

¹H NMR (CD₃OD, 400 MHz) δ 0.32-0.44 (m, 2H), 0.45-0.58 (m, 2H), 0.88 (s, 3H), 0.92-1.01 (m, 1H), 1.07 (s, 3H), 1.17-1.30 (m, 1H), 2.64 (dd, 1H, J=8.7, 17.3), 2.74 (d, 1H, J=8.4), 2.91 (d, 1H, J=8.8), 3.02 (dd, 1H, J=3.8, 13.8), 3.15-3.28 (m, 2H), 3.42-3.68 (m, 10H), 4.11 (dd, 1H, J=4.6, 9.8), 6.27 (dd, 1H, J=4.4, 8.0), 6.56-6.63 (m, 2H), 7.44 (d, 2H, J=8.0), 7.50 (d, 2H, J=8.4); HPLC-MS calcd. for C₃₀H₃₅F₄N₃O₃ (M+H⁺) 562.3, found 562.5.

Example 70 N-[1-(S)-Cyclopropyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide

¹H NMR (400 MHz, MeOD) δ 7.43 (d, J=8.5 Hz, 2H), 7.40 (d, J=8.6 Hz, 2H), 6.74-6.79 (m, 2H), 6.30-6.36 (m, 2H), 4.00 (dd, J=10.1, 4.6 Hz, 1H), 3.30-3.57 (m, 8H), 3.17-3.26 (m, 1H), 3.00-3.15 (m, 3H), 2.53 (dd, J=16.2, 4.6 Hz, 1H), 0.80-0.90 (m, 1H), 0.38-0.45 (m, 1H), 0.28-0.36 (m, 1H), 0.13-0.23 (m, 2H); HPLC-MS calcd. for C₂₇H₂₉F₆N₃O₄ (M+H⁺) 574.5, found 574.4.

Example 71 2-(R)-(4-methanesulfonyl-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide

Compound is synthesized in accordance with Example 21 and isolated as a minor diastereomer. HPLC-MS calcd. for C₂₇H₃₄F₃N₃O₆S (M+H⁺) 586.2, found 586.4.

Example 72 (S,S)-Morpholine-4-carboxylic Acid {2-cyclohexyl-1-[2-(4-methoxy-phenylamino)-1-methyl-ethylcarbamoyl]-ethyl}-amide

¹H NMR (CD₃OD, 400 MHz) δ 7.02 (d, J=8.6 Hz, 2H), 6.75 (dt, J=9.1, 3.5 Hz, 2H), 4.2 (dd, J=9, 6.8 Hz, 1H), 4.08 (dt, J=13, 6.8 Hz, 1H), 3.6 (m, 4H), 3.36 (m, 4H), 3.15 (m, 2H), 1.68 (m, 5H), 1.49 (m, 2H), 1.18 (d, J=6.8 Hz, 3H), 1.16 (m, 3H), 0.89 (m, 2H). HPLC-MS for C₂₄H₃₈N₄O₄ (M+1)=447.3.

Example 73 Morpholine-4-carboxylic Acid {2-cyclopentyl-1-(S)-[2-(4-methoxy-phenylamino)-1-(S)-methyl-ethylcarbamoyl]-ethyl}-amide

HPLC-MS for C₂₃H₃₆N₄O₄ (M+1)=433.3.

Example 74 Morpholine-4-carboxylic Acid (2-cyclohexyl-1-(S)-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide

Compound is synthesized in accordance with Example 17. HPLC-MS calcd. for C₂₆H₃₉F₃N₄O₆S (M+H⁺) 593.3, found 593.5.

Example 75 Morpholine-4-carboxylic Acid (2-cyclohexyl-1(S)-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide

Compound is synthesized in accordance with Example 17. HPLC-MS calcd. for C₂₅H₃₇F₃N₄O₄ (M+H⁺) 515.3, found 515.5.

Example 76 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[2-methyl-1-(S)-(pyridin-3-ylaminomethyl)-propylcarbamoyl]-ethyl}-amide

Compound is synthesized in accordance with Example 64. HPLC-MS calcd. for C₂₄H₃₉N₅O₃ (M+H⁺) 446.3, found 446.4.

Example 77 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[1-(S)-methyl-2-(5-methyl-isoxazol-3-ylamino)-ethylcarbamoyl]-ethyl}-amide; C₂₁H₃₅N₅O₄; HPLC-MS: 422.5 (M+H⁺).

Example 78 Morpholine-4-carboxylic Acid {1-(S)-[2-(benzothiazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide; C₂₄H₃₅N⁵O₃S; HPLC-MS: 474.5 (M+H⁺)

Example 79 Morpholine-4-carboxylic Acid {1-(S)-[2-(benzooxazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide

C₂₄H₃₅N₅O₄; HPLC-MS: 458.5 (M+H⁺).

Example 80 (S,S)-Morpholine-4-carboxylic Acid {2-cyclohexyl-1-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethylcarbamoyl]-ethyl}-amide

HPLC-MS for C₂₇H₄₁FN₄O₃ (M+1)=489.3.

Example 81 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide

¹H NMR (400 MHz, MeOD) δ 7.85 (d, J=8.8 Hz, 1H), 6.70 (m, 2H), 6.44 (m, 1H), 6.26 (d, J=8.0 Hz, 1H), 4.27 (m, 1H), 3.62 (m, 3H), 3.53 (m, 1H), 3.26-3.38 (m, 7H), 3.09 (dd, J=8.4, 3.1 Hz, 1H), 3.04 (d, J=8.4 Hz, 1H), 1.57-1.76 (m, 5H), 1.27 (s, 3H), 1.23 (s, 3H), 1.04-1.50 (m, 6H), 0.96 (m, 1H), 0.76-0.89 (m, 2H), 0.56 (m, 1H), 0.45 (m, 1H), 0.34 (m, 2H); HPLC-MS calcd. for C₂₉H₄₃FN₄O₃ (M+H⁺) 515.7, found 515.5.

Example 82 Morpholine-4-carboxylic Acid {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-spirocyclopropyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide

¹H NMR (400 MHz, MeOD) δ 7.85 (d, J=8.7 Hz, 1H), 6.65 (ddd, J=9.3, 9.3, 2.6 Hz, 1H), 6.40 (dd, J=8.5, 4.1 Hz, 1H), 6.31 (dd, J=8.5, 2.6 Hz, 1H), 6.23 (d, J=7.9 Hz, 1H), 4.26 (m, 1H), 3.62 (m, 4H), 3.50 (m, 2H), 3.36 (m, 10H), 3.14 (dd, J=13.8, 4.6 Hz, 1H), 1.66 (m, 4H), 1.43 (m, 1H), 1.34 (m, 1H), 1.15 (m, 2H), 0.819-1.08 (m, 6H), 0.56 (m, 1H), 0.46 (m, 1H), 0.34 (m, 2H); HPLC-MS calcd. for C₂₉H₄₂FN₄O₃ (M+H⁺) 513.7, found 513.5.

Example 83 [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

HPLC-MS calcd. for C₂₄H₃₅F₃N₃O₅ (M+H⁺) 502.3, found 502.4.

Example 84 [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic Acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl Ester

¹H NMR (400 MHz, CDCl₃) δ 7.00 (d, J=8.6 Hz, 2H), 6.56 (d, J=8.7 Hz, 2H), 5.38 (d, J=9.9 Hz, 1H), 4.04-4.20 (m, 1H), 3.87-3.97 (m, 1H), 3.42-3.80 (m, 8H), 3.10-3.20 (m, 1H), 1.81 (dd, J=15.0, 10.1 Hz, 1H), 1.42-1.50 (m, 1H), 1.24 (d, J=6.7 Hz, 3H), 0.95 (s, 9H); HPLC-MS calcd. for C₂₂H₃₃F₃N₃O₅ (M+H⁺) 476.5, found 476.3.

Example 85 [2-(4-Difluoromethoxy-phenylamino)-1-(S)-methyl-ethyl]-carbamic Acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl Ester

¹H NMR (400 MHz, CDCl₃) δ 6.92-6.96 (m, 2H), 6.53-6.58 (m, 2H), 6.358 (dd, J=42.6, 42.6 Hz, 1H), 5.35-5.41 (m, 1H), 3.98-4.13 (m, 1H), 3.84-3.98 (m, 1H), 3.40-3.80 (m, 8H), 3.10-3.20 (m, 1H), 1.81 (dd, J=14.9, 10.2 Hz, 1H), 1.42-1.50 (m, 1H), 1.24 (d, J=6.7 Hz, 3H), 0.95 (s, 9H); HPLC-MS calcd. for C₂₂H₃₄F₂N₃O₅ (M+H⁺) 458.5, found 458.3.

Example 86 {2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

Compound is synthesized as outlined in example 5. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₅ (M+H⁺) 530.3, found 530.5.

Example 87 {3-Phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

Compound is synthesized as outlined in example 5. HPLC-MS calcd. for C₃₁H₄₀F₃N₃O₅ (M+H⁺) 592.3, found 592.6.

Example 88 {1-(S)-[(4-Trifluoromethoxy-phenylamino)-methyl]-butyl}-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

Compound is synthesized as outlined in example 5. HPLC-MS calcd. for C₂₆H₃₈F₃N₃O₅ (M+H⁺) 530.3, found 530.5.

Example 89 [2-(4-Acetylsulfamoyl-phenylamino)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester;

-   -   C₂₅H₃₈N₄O₇S; HPLC-MS: 539.5 (M+H⁺).

Example 90 [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester;

-   -   C₂₇H₄₀FN₃O₄; HPLC-MS: 490.6 (M+H⁺).

Example 91 [1-(S)-Methyl-2-(5-methyl-isoxazol-3-ylamino)-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

-   -   C₂₁H₃₄N₄O₅; HPLC-MS: 423.5 (M+H⁺).

Example 92 (S,S)-[1-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl-methyl)-propyl]-carbamic Acid 1-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

¹H NMR (CDCl₃, 400 MHz) δ 6.74 (m, 2H), 6.38 (s, 1H), 5.33 (d, J=7.86 Hz, 1H), 4.98 (s, 1H), 3.94 (m, 1H), 3.68 (m, 5H), 3.57 (m, 3H), 3.22 (d, J=8.35 Hz, 1H), 3.22 (d, J=8.35 Hz, 1H), 3.1 (m, 2H), 2.99 (dd, J=13.6, 6.8 Hz, 1H), 1.7 (m, 7H), 1.44 (m, 2H), 1.26 (m, 10H), 1.14 (m, 3H), 0.91 (m, 2H).HPLC-MS for C₂₇H₄₁FN₄O₃ (M+1)=504.3. (118636).

Example 93 [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl Ester

C₂₉H₄₄FN₃O₄; HPLC-MS: 518.6 (M+H⁺); ¹H-NMR (400 MHz) □ (DMSO-D₆) 7.23(m, 1H), 6.79(m, 1H), 6.68(m, 1H), 6.35(m, 1H), 5.19(m, 1H), 4.11(m, 1H), 3.66(m, 2H), 2.79(m, 1H), 2.62(m, 4H), 2.19(m, 1H), 1.70(m, 1H), 1.53(m, 4H); 1.24(m, 3H); 1.02(m, 18H), 0.88(m, 2H).

Example 94 (S,S)-[2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethyl]-carbamic Acid 2-(4-acetyl-piperazin-1-yl)-1-cyclohexylmethyl-2-oxo-ethyl Ester; C₂₉H₄₃FN₄O₄; HPLC-MS: 531.7 (M+H⁺)

Example 95 [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl Ester; C₂₈H₄₃FN₄O₅S; HPLC-MS: 567.6 (M+H⁺)

Example 96 [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl Ester; C₂₇H₄₀FN₃O₃S; HPLC-MS: 506.5 (M+H⁺)

Example 97 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl Ester; C₂₉H₄₄FN₃O₄; HPLC-MS: 518.6 (M+H⁺)

Example 98 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 2-(4-acetyl-piperazin-1-yl)-1-(S)-cyclohexylmethyl-2-oxo-ethyl Ester; C₂₉H₄₃FN₄O₄; HPLC-MS: 531.7 (M+H⁺)

Example 99 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl Ester; C₂₈H₄₃FN₄O₅S; HPLC-MS: 567.6 (M+H⁺)

Example 100 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl Ester; C₂₇H₄₀FN₃O₃S; HPLC-MS: 506.5 (M+H⁺).

Example 101 [2-(5-Fluoro-3,3-spiro-cylopropyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

¹H NMR (400 MHz, MeOD) δ 6.66 (m, 1H), 6.41 (dd, J=8.5, 4.1 Hz, 1H), 6.32 (dd, J=8.5, 2.5 Hz, 1H), 5.24 (m, 1H), 3.83 (m, 1H), 3.45-3.77 (m, 10H), 3.41 (d, J=8.6 Hz, 1H), 3.15 (dd, J=13.7, 7.0 Hz, 1H), 3.00 (dd, J=13.7, 5.8 Hz, 1H), 1.59-1.81 (m, 6H), 1.37-1.50 (m, 2H), 1.21 (d, J=6.8 Hz. 3H), 1.12-1.26 (m, 3H), 0.95 (m, 6H); HPLC-MS calcd. for C₂₇H₃₉FN₃O₄ (M+H⁺) 488.6, found 488.4.

Example 102 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-oxo-2-piperidin-1-yl-ethyl Ester; C₂₈H₄₂FN₃O₃; HPLC-MS: 488.5 (M+H⁺)

Example 103 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-oxo-2-pyrrolidin-1-yl-ethyl Ester; C₂₇H₄₀FN₃O₃; HPLC-MS: 474.5 (M+H⁺)

Example 104 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 2-cyclohexyl-1-(S)-dimethylcarbamoyl-ethyl Ester; C₂₅H₃₈FN₃O₃; HPLC-MS: 448.5 (M+H⁺)

Example 105 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-(1,1-dioxo-1 λ⁶-thiomorpholin-4-yl)-2-oxo-ethyl Ester; C₂₇H₄₀FN₃O₅S; HPLC-MS: 538.5 (M+H⁺)

Example 106 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 2-cyclohexyl-1-(S)-[(2-methoxy-ethyl)-methyl-carbamoyl]-ethyl Ester; C₂₇H₄₂FN₃O₄; HPLC-MS: 491.5 (M+H⁺)

Example 107 [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic Acid 2-azetidin-1-yl-1-(S)-cyclohexylmethyl-2-oxo-ethyl Ester; C₂₆H₃₈FN₃O₃; HPLC-MS: 460.5 (M+H⁺)

Example 108 [1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-carbamic Acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl Ester

¹H NMR (400 MHz, CDCl₃) δ 6.67-6.74 (m, 2H), 6.34-6.41(m, 1H), 5.27-5.33 (m, 1H), 5.03-5.09 (m, 1H), 3.40-3.75 (m, 8H), 3.13-3.26 (m, 4H), 1.55-1.81 (m, 6H), 1.30-1.46 (m, 2H), 1.26 (s, 3H), 1.24 (s, 3H), 1.03-1.25 (m, 3H), 0.78-0.99 (m, 3H), 0.43-0.58 (m, 2H), 0.27-0.41 (m, 2H); HPLC-MS calcd. for C₂₉H₄₃FN₃O₄ (M+H⁺) 516.3, found 516.5.

Example 109 [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic Acid 2-morpholin-4-yl-2-oxo-1-(R,S)-phenyl-ethyl Ester

Compound is synthesized as outlined in example 5 using (L)-mandelic acid. The compound is isolated as a mixture of diastereomers. HPLC-MS calcd. for C₂₈H₃₆FN₃O₄ (M+H⁺) 498.3, found 498.3.

Example 110 [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic Acid 2-morpholin-4-yl-2-oxo-1-(R)-phenylmethanesulfonylmethyl-ethyl Ester

Step A: This reaction was performed as previously described by Deechongkit, S.; You, S.-L.; Kelly, J. W. Org. Lett. 2004, 6, 497, using (S)-Methylglycidate 110a and Benzyl mercaptan. (R)-3-Benzylsulfanyl-2-hydroxy-propionic acid methyl ester 110b (7.41 g, 31.41 mmol, 92%) was isolated as a viscous oil: MS calcd. for C₁₁H₁₄O₃S (M+H⁺) 227.1, found 227.3.

Step B: This reaction was performed as previously described by Deechongkit, S.; You, S.-L.; Kelly, J. W. Org. Lett. 2004, 6, 497, using (R)-3-Benzylsulfanyl-2-hydroxy-propionic acid methyl ester 110b and lithium hydroxide. (R)-3-Benzylsulfanyl-2-hydroxy-propionic acid 110c (3.08 g, 14.51 mmol, 46%) was isolated as a viscous oil: MS calcd. for C₁₀H₁₂O₃S (M+Na⁺) 235.1, found 235.3.

Step C: This reaction was performed as previously described in example 5, using (R)-3-Benzylsulfanyl-2-hydroxy-propionic acid 110c. (R)-3-Benzylsulfanyl-2-hydroxy-1-morpholin-4-yl-propan-1-one 110d (3.41 g, 11.87 mmol, 67%) was isolated as a viscous oil: MS calcd. for C₁₄H₁₉NO₃S (M+H⁺) 282.1, found 282.4.

Step D: Oxone (2 KHSO₅.KHSO₄.K₂SO₄) (10.55 g, 17.17 mmol, 3.0 eq.) was dissolved in H₂O (25 mL, 0.7 M) and added to a MeOH (25 mL, 0.3 M) solution of (R)-3-Benzylsulfanyl-2-hydroxy-1-morpholin-4-yl-propan-1-one 110d (1.61 g, 5.73 mmol, 1.0 eq.) at 0° C. over a 30 minute period. The reaction was monitored to completion by LC/MS. After the reaction was judged to be complete (˜12 h), the MeOH was evaporated in vacuo. The resulting solution was diluted with H₂O (30 mL) and extracted with CH₂Cl₂ (3×50 mL). The organic extracts were combined, washed with H₂O (75 mL) and saturated NaCl (50 mL). The organic layer was dried over MgSO₄ and filtered. The organic solvent was removed in vacuo and provided (R)-2-Hydroxy-1-morpholin-4-yl-3-phenylmethanesulfonyl-propan-1-one 110e as a viscous oil (1.60 g, 5.11 mmol, 89%) which was used directly without further purification: MS calcd. for C₁₄H₁₉NO₅S (M+H⁺) 314.1, found 314.3.

Step E: This reaction was performed as previously described example 5, using (R)-2-Hydroxy-1-morpholin-4-yl-3-phenylmethanesulfonyl-propan-1-one 110e. (R)-Carbonic acid 2-morpholin-4-yl-2-oxo-1-phenylmethanesulfonylmethyl-ethyl ester 4-nitro-phenyl ester 110f (1.98 g, 4.14 mmol, 81%) was isolated as a white solid after column chromotography: MS calcd. for C₂₁H₂₂N₂O₉S (M+H⁺) 479.1, found 479.3.

From the mixed carbonate, the title compound is prepared according to the procedures described in example 5 and isolated as a white solid: HPLC-MS calcd. for C₃₀H₄₀FN₃O₆S (M+H⁺) 590.3, found 590.2.

Example 111 [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic Acid 1-(S)-cyclohexyl-2-morpholin-4-yl-2-oxo-ethyl Ester

Compound is synthesized as outlined in example 5 using (L)-tetrahydromandelic acid. HPLC-MS calcd. for C₂₈H₄₂FN₃O₄ (M+H⁺) 504.3, found 504.6.

B. Assays for Cathepsin Inhibitory Activity

Cathepsin S

The optimal substrate for cathepsin S, acetyl-histidine-proline-valine-lysine-amino carbamoyl coumarin, was identified from screening a combinatorial library of fluorogenic peptide substrates (Harris, J. L., B. J. Backes, et al., Proc Natl Acad Sci USA 2000, 97(14), 7754-9). Kinetic measurements are performed in a total reaction volume of 30 μl in 384-well microtiter plates. Cathepsin S, at a final concentration of 0.3-3 nM (active site), is incubated with the compounds at twelve varying concentrations in a buffer containing 100 mM NaAc (pH5.5), 1 mM EDTA, 100 mM NaCl, 0.01% Brij-35 for 20 minutes at room temperature. Control reactions in the absence of inhibitor are performed in replicates of 24. The reactions are initiated by adding the substrate, acetyl-histidine-proline-valine-lysine-amino carbamoyl coumarin, to a final concentration of 50 μM. The rate of substrate hydrolysis is measured by monitoring the increase in fluorescence at an excitation wavelength of 380 nm and an emission wavelength of 450 nm that results from cleavage of the aniline bond in the substrate by the enzyme. The apparent inhibition constants for the compounds are determined from the enzyme progress curves (Kuzmic, P., K. C. Elrod, et al., Anal Biochem 2000, 286(1), 45-50) and are then used to calculate the inhibition constants for competitive inhibitors.

Cathepsin K

The optimal substrate for cathepsin K, acetyl-lysine-histidine-proline-lysine-amino carbamoyl coumarin, was identified from screening a combinatorial library of fluorogenic peptide substrates (Harris, J. L., B. J. Backes, et al., Proc Natl Acad Sci USA 2000, 97(14), 7754-9). Kinetic measurements are performed in a total reaction volume of 30 μl in 384-well microtiter plates. Cathepsin K, at a final concentration of 3.5 nM (active site), is incubated with the compounds at twelve varying concentrations in a buffer containing 100 mM NaAc (pH5.5), 1 mM EDTA, 100 mM NaCl, 0.01% Brij-35 for 20 minutes at room temperature. Control reactions in the absence of inhibitor are performed in replicates of 24. The reactions are initiated by adding the substrate, acetyl-lysine-histidine-proline-lysine-amino carbamoyl coumarin, to a final concentration of 40 μM. The rate of substrate hydrolysis is measured by monitoring the increase in fluorescence at an excitation wavelength of 380 nm and an emission wavelength of 450 nm that results from cleavage of the aniline bond in the substrate by the enzyme. The apparent inhibition constants for the compounds are determined from the enzyme progress curves (Kuzmic, P., K. C. Elrod, et al., Anal Biochem 2000, 286(1), 45-50) and are then used to calculate the inhibition constants for competitive inhibitors.

Cathepsin L

The optimal substrate for cathepsin L, acetyl-histidine-lysine-phenylalanine-lysine-amino carbamoyl coumarin, was identified from screening a combinatorial library of fluorogenic peptide substrates (Harris, J. L., B. J. Backes, et al., Proc Natl Acad Sci USA 2000, 97(14), 7754-9). Kinetic measurements are performed in a total reaction volume of 30 μl in 384-well microtiter plates. Cathepsin L, at a final concentration of 0.1 nM (active site), is incubated with the compounds at twelve varying concentrations in a buffer containing 100 mM NaAc (pH5.5), 1 mM EDTA, 100 mM NaCl, 0.01% Brij-35 for 20 minutes at room temperature. Control reactions in the absence of inhibitor are performed in replicates of 24. The reactions are initiated by adding the substrate, acetyl-histidine-lysine-phenylalanine-lysine-amino carbamoyl coumarin, to a final concentration of 20 μM. The rate of substrate hydrolysis is measured by monitoring the increase in fluorescence at an excitation wavelength of 380 nm and an emission wavelength of 450 nm that results from cleavage of the aniline bond in the substrate by the enzyme. The apparent inhibition constants for the compounds are determined from the enzyme progress curves (Kuzmic, P., K. C. Elrod, et al., Anal Biochem 2000, 286(1), 45-50) and are then used to calculate the inhibition constants for competitive inhibitors.

Cathepsin B

The optimal substrate for cathepsin B, acetyl-histidine-proline-valine-lysine-amino carbamoyl coumarin, was identified from screening a combinatorial library of fluorogenic peptide substrates (Harris, J. L., B. J. Backes, et al., Proc Natl Acad Sci USA 2000, 97(14), 7754-9). Kinetic measurements are performed in a total reaction volume of 30 μl in 384-well microtiter plates. Cathepsin B, at a final concentration of 1.5 nM (active site), is incubated with the compounds at twelve varying concentrations in a buffer containing 100 mM NaAc (pH 5.5), 1 mM EDTA, 100 mM NaCl, 0.01% Brij-35 for 20 minutes at room temperature. Control reactions in the absence of inhibitor are performed in replicates of 24. The reactions are initiated by adding the substrate, acetyl-histidine-proline-valine-lysine-amino carbamoyl coumarin, to a final concentration of 10 μM. The rate of substrate hydrolysis is measured by monitoring the increase in fluorescence at an excitation wavelength of 380 nm and an emission wavelength of 450 nm that results from cleavage of the aniline bond in the substrate by the enzyme. The apparent inhibition constants for the compounds are determined from the enzyme progress curves (Kuzmic, P., K. C. Elrod, et al., Anal Biochem 2000, 286(1), 45-50) and are then used to calculate the inhibition constants for competitive inhibitors.

Preferred cathepsin S inhibition constants for compounds of the present invention are less than 10 μM. More preferred inhibition constants for compounds of the present invention are less than 1.0 μM. Most preferred inhibition constants for compounds of the present invention are less than 0.1 μM.

Selectivity for cathepsin S in the presence of cathepsin isozymes was determined by the ratio of the cathepsin isozyme inhibition constant of a compound of the present invention to the cathepsin S inhibition constant of the same compound. Preferred compounds of the present invention selective for cathepsin S have ratios of greater than 10. More preferred compounds of the present invention selective for cathepsin S have ratios of greater than 100. Most preferred compounds of the present invention selective for cathepsin S have ratios of greater than 1000. TABLE I Assay Data for Inhibitors of Cathepsin S Selectivity Selectivity Selectivity K_(i) for Cat. S for Cat. S for Cat. S Example Cat. S^(a) over Cat. K^(b) over Cat. L^(b) over Cat. B^(b) 1 + − − + 2 +++ ++ + +++ 3 +++ ++ + ++ 4 +++ ++ + +++ 5 + + + + 6 + + + + 7 + + + + 8 +++ ++ ++ +++ 9 + + + + 10 +++ ++ +++ +++ 11 ++ ++ ++ ++ 12 ++ + + ++ 13 ++ ++ ++ ++ 14 +++ ++ ++ +++ 15 +++ ++ ++ +++ 16 ++ + + ++ 17 +++ ++ + ++ 18 +++ +++ ++ ++ 19 ++ ++ ++ ++ 20 +++ + + +++ 21 +++ ++ +++ +++ 22 +++ + + +++ 23 +++ + − ++ 24 +++ − + +++ 25 ++ ++ ++ ++ 26 ++ − ++ ++ 27 +++ + ++ +++ 28 +++ + + +++ 29 +++ + + ++ 30 +++ ++ + ++ 31 +++ + ++ +++ 32 +++ − + +++ 33 +++ +++ + +++ 34 +++ + + +++ 35 +++ + ++ +++ 36 +++ ++ + +++ 37 +++ ++ + ++ 38 +++ − − +++ 39 +++ + ++ +++ 40 +++ + ++ +++ 41 +++ + ++ +++ 42 +++ +++ ++ +++ 43 ++ ++ ++ ++ 44 ++ + ++ ++ 45 +++ ++ ++ +++ 46 +++ +++ ++ +++ 47 ++ − + + 48 ++ + ++ ++ 49 + + + + 50 +++ ++ − +++ 51 +++ ++ + +++ 52 ++ + ++ ++ 53 ++ ++ ++ ++ 54 +++ +++ ++ +++ 55 ++ +++ ++ ++ 56 − − − − 57 ++ ++ ++ ++ 58 ++ ++ + ++ 59 + + + + 60 + + + + 61 + + + + 62 ++ ++ ++ ++ 63 ++ ++ ++ ++ 64 ++ + + ++ 65 ++ ++ ++ ++ 66 + − − + 67 + + + + 68 +++ ++ +++ +++ 69 ++ + ++ ++ 70 ++ ++ ++ ++ 71 + − ++ + 72 +++ ++ + ++ 73 +++ + + ++ 74 +++ + − + 75 +++ + − + 76 ++ + − + 77 ++ + + ++ 78 + + − + 79 + + − + 80 +++ +++ + +++ 81 +++ ++ + +++ 82 +++ ++ − ++ 83 ++ ++ ++ ++ 84 + − + + 85 + − + + 86 +++ ++ ++ +++ 87 +++ ++ ++ +++ 88 +++ + ++ +++ 89 + + + + 90 ++ ++ ++ ++ 91 + + + + 92 +++ +++ +++ +++ 93 + + − + 94 + + + + 95 + + + + 96 + + + + 97 + + − + 98 + + + + 99 + + + + 100 + + + + 101 ++ ++ ++ ++ 102 +++ +++ +++ +++ 103 +++ ++ +++ +++ 104 ++ ++ ++ ++ 105 ++ + + ++ 106 ++ ++ ++ ++ 107 ++ ++ ++ ++ 108 +++ +++ +++ +++ 109 + − + + 110 + + + + 111 +++ + ++ +++ ^(a)Cathepsin S inhibition constant for compounds of Formula I: +, <10 μM; ++, <1.0 μM; +++, <0.1 μM. ^(b)Selectivity of compounds of Formula I for cathepsin S over another cathepsin: +, >10; ++, >100; +++, >1000.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference. 

1. A compound having the Formula I:

or a pharmaceutically acceptable salt or prodrug thereof, wherein: Q is a heterocycle selected from the group consisting of azetidinyl, pyrrolidinyl, piperidyl, morpholinyl, thiomorpholinyl, piperazinyl and indolinyl substituted with 0-2 R^(Q), wherein Q is connected to —C(═O)— via a ring nitrogen atom; and NR²⁵R²⁶; each R^(Q) is independently a member selected from the group consisting of OH, F, Cl, —S(═O)₂CH₃—, acetyl, ═O, C₁-C₆ alkyl, C₁-C₆ alkoxy, CF₃, OCF₃ and NR¹⁰R¹¹; A is a member selected from the group consisting of —O—CR¹R²—, —NH—CR¹R²—, —CR³R⁴—O—, and —CR³R⁴—CR¹R²—; each of R¹ and R³ is independently a member selected from the group consisting of H, a C₁-C₆ alkoxy, a C₁-C₆ alkyl substituted with 0-2 R^(1a), wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)— and —S(═O)₂—; a C₂-C₆ alkenyl, a C₃-C₆ alkynyl, a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heteroaryl is substituted with 0-3 R¹³; each R^(1a) is independently a member selected from the group consisting of a C₆-C₁₀ aryl substituted with 0-3 R¹³, a 5- to 6-membered monocyclic or 8- to 10-membered bicyclic heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heteroaryl is substituted with 0-3 R¹³, a C₃-C₈ cycloalkyl substituted with 0-2 R^(Q), a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q), and a C₁-C₃ perfluoroalkyl; each of R² and R⁴ is independently a member selected from the group consisting of H, F, OH, C₁-C₆ alkyl and C₁-C₆ alkoxy; R⁵ is a member selected from the group consisting of H, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, phenyl substituted with 0-2 R¹³, and a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heteroaryl is substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-2 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and —NR²²—; each of R⁶, R⁷, R⁸ and R⁹ is independently a member selected from the group consisting of H and C₁-C₆ alkyl; alternatively, R⁵ and R⁷ are taken together to form a C₅-C₇ cycloalkyl, wherein a methylene of said C₅-C₇ cycloalkyl may optionally be replaced with a heteroatom selected from the group of —O—, —S—, —S(═O)—, and —S(═O)₂—; each R¹⁰ is independently a member selected from the group consisting of H, C₁-C₄ alkyl, (C₁-C₄ alkyl)-C(═O)— and (C₁-C₄ alkyl)-S(═O)₂—; each R¹¹ is independently a member selected from the group consisting of H and C₁-C₄ alkyl; each R¹² is independently a member selected from the group consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3 R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said 5- to 6-membered heteroaryl is substituted with 0-3 R¹³, and a C₁-C₆ alkyl substituted with 0-1 R¹⁹; each R¹³ is independently a member selected from the group consisting of H, OH, F, Cl, Br, CN, NO₂, COOR¹⁷, C(═O)NR¹⁷R¹⁸, S(═O)₂NR¹⁷R¹⁸, acetyl, —SCH₃, —S(═O)CH₃, —S(═O)₂CH₃, —NR¹⁰R¹¹, C₁-C₆ alkoxy, C₁-C₃ perfluoroalkyl, C₁-C₃ perfluoroalkoxy and a C₁-C₆ alkyl; each R¹⁴ is independently a member selected from the group consisting of H, C₃-C₇ cycloalkyl, C₁-C₄ alkyl substituted with 0-1 R¹⁹, and a phenyl substituted with 0-3 R¹³; each R¹⁵ is independently a member selected from the group consisting of H, C₃-C₈ cycloalkyl, a phenyl substituted with 0-3 R¹³, and a C₁-C₆ alkyl substituted with 0-1 R¹⁹; each R¹⁶ is independently a member selected from the group consisting of H and C₁-C₄ alkyl; alternatively, R¹⁵ and R¹⁶ on the same N atom are taken together to form a C₅-C₇ heterocycle containing 1-2 heteroatoms each independently a member selected from the group consisting of N, O and S; each of R¹⁷ and R¹⁸ is independently a member selected from the group consisting of H, C₁-C₄ alkyl and C₃-C₆ cycloalkyl; each R¹⁹ is independently a member selected from the group consisting of H, C₃-C₇ cycloalkyl, a phenyl substituted with 0-3 R¹³ and a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said 5- to 6-membered heteroaryl is substituted with 0-3 R¹³; Ar is a member selected from the group consisting of phenyl substituted with 0-3 R²⁰, and a 5- to 10-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S; wherein said heteroaryl is substituted with 0-3 R²⁰; each R²⁰ is independently a member selected from the group consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃, S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, —S(═O)₂NH(C═O)CH₃, C(═O)NR¹⁷R¹⁸, CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃ and OCF₂H; alternatively, R²⁰ and R⁹ are taken together to form a 5- to 7-membered heterocyclic ring containing 1-2 heteroatoms each independently a member selected from the group consisting of N, O and S; wherein said 5 to 7 membered heterocyclic ring is ortho-fused to Ar; wherein said 5- to 7-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴; each R²¹ is independently a member selected from the group consisting of H, OH, F, Cl, CN, NO₂, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, C₁-C₃ perfluoroalkoxy, C₁-C₄ alkoxy, C₂-C₄ alkenyl, C₂-C₄ alkynyl, phenyl substituted with 0-3 R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heteroaryl is substituted with 0-3 R¹³, C₃-C₈ heterocycle containing 1 to 2 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heterocycle is substituted with 0-2 R¹³ and is saturated or partially unsaturated, and C₃-C₈ cycloalkyl; R²² is independently a member selected from the group consisting of H, ^(t)BOC, Cbz, C₃-C₈ cycloalkyl, (C₁-C₆ alkyl)-C(═O)—, (C₁-C₆ alkyl)-S(═O)₂—, a C₁-C₆ alkyl substituted with 0-1 R¹⁹, a phenyl substituted with 0-3 R¹³ and a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said 5- to 6-membered heteroaryl is substituted with 0-3 R¹³; each R²³ is independently a member selected from the group consisting of H and C₁-C₄ alkyl. each R²⁴ is independently a member selected from the group consisting of C₁-C₄ alkyl, F, Cl and C₁-C₄ alkoxy, CF₃ and OCF₃; alternatively, two R²⁴ may be combined to form C₃-C₆ cycloalkyl. each of R²⁵ and R²⁶ is independently a member selected from the group consisting of C₁-C₆ alkyl, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)—, —S(═O)₂— and —NR₂₂—.
 2. The compound of claim 1, wherein said compound has the formula:

wherein: R¹ is independently a member selected from the group consisting of H, C₁-C₆ alkyl substituted with 0-1 R^(1a), wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, and —S(═O)₂, a C₂-C₆ alkenyl, a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³, a 5- to 6-membered heteroaryl containing 1 to 4 heteroatoms each independently a member selected from the group consisting of N, O and S, wherein said heteroaryl is substituted with 0-3 R¹³; R⁴ is a member selected from the group consisting of H, F, OH and C₁-C₆ alkyl.
 3. The compound of claim 1, wherein said compound has the formula:

wherein: R¹ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄ alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S— and —S(═O)₂—; R^(1a) is a member selected from the group consisting of a phenyl substituted with 0-3 R¹³, a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q).
 4. The compound of claim 1, wherein said compound has the formula:

wherein: R³ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄ alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S— and —S(═O)₂—; a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³; R^(1a) is a member selected from the group consisting of a phenyl substituted with 0-3 R¹³, a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q).
 5. The compound of claim 1, wherein said compound has the formula:

wherein: R¹ is selected from the group consisting of C₁-C₆ alkyl, C₁-C₄ alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, and —S(═O)₂; R^(1a) is a member selected from the group consisting of a phenyl substituted with 0-3 R¹³, C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁ bicycloalkyl substituted with 0-2 R^(Q); R⁵ is a member selected from the group consisting of H, phenyl substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)₂—; each R²¹ is independently a member selected from the group consisting of H, OH, F, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, phenyl substituted with 0-3 R¹³, and C₃-C₇ cycloalkyl; Ar is a member selected from the group consisting of phenyl substituted with 0-3 R²⁰; each R²⁰ is independently a member selected from the group consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃, S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, C(═O)NR¹⁷R¹⁸, CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃; alternatively, R²⁰ and R⁹ are taken together to form a 5-membered heterocyclic ring containing 1 nitrogen, wherein said 5-membered heterocyclic ring is ortho-fused to Ar; wherein said 5-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴.
 6. The compound of claim 1, wherein said compound has the formula:

wherein: R² is H; R³ are each independently selected from the group consisting of H, C₁-C₆ alkyl, C₁-C₄ alkyl substituted with 1 R^(1a), R^(1a) is a member selected from the group consisting of a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); R⁵ is a member selected from the group consisting of H, phenyl substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)₂—; each R²¹ is independently a member selected from the group consisting of H, OH, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, phenyl substituted with 0-3 R¹³, and C₃-C₈ cycloalkyl; Ar is a member selected from the group consisting of phenyl substituted with 0-3 R²⁰, each R²⁰ is independently a member selected from the group consisting of H, F, Cl, Br, CN, OR¹², SCH₃, S(═O)CH₃, S(═O)₂CH₃, S(═O)₂NR¹⁷R¹⁸, NR¹⁰R¹¹, acetyl, —S(═O)₂NH(C═O)CH₃, C(═O)NR¹⁷R¹⁸, CO₂R¹⁷, C(═NH)NH₂, C₁-C₆ alkyl, CF₃, OCF₃ and OCF₂H; alternatively, R²⁰ and R⁹ are taken together to form a 5-membered heterocyclic ring containing 1 nitrogen; wherein said 5-membered heterocyclic ring is ortho-fused to Ar; wherein said 5-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴.
 7. The compound of claim 2, wherein: R⁵ is a member selected from the group consisting of H, phenyl substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)₂—; each R²¹ is independently a member selected from the group consisting of H, OH, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, phenyl substituted with 0-3 R¹³, and C₃-C₇ cycloalkyl.
 8. The compound of claim 7, wherein: R¹ is independently a member selected from the group consisting of C₁-C₄ alkyl substituted with 1 R^(1a), wherein said C₁-C₄ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, and —S(═O)₂; C₁-C₆ alkyl, a C₂-C₆ alkenyl, a C₃-C₇ cycloalkyl substituted with 0-2 R^(Q), and a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); phenyl substituted with 0-3 R¹³; each R^(1a) is independently a member selected from the group consisting of a phenyl substituted with 0-3 R¹³, a C₃-C₈ cycloalkyl substituted with 0-2 R^(Q), a C₇-C₁₁ bicycloalkyl substituted with 0-2 R^(Q); R⁴ is H, F, OH, methyl, ethyl, propyl or butyl; Ar is a phenyl substituted with 0-3 R²⁰; a 5- to 6-membered heteroaryl containing 1 to 2 heteroatoms each independently a member selected from the group consisting of N, O and S; wherein said heteroaryl is substituted with 0-2 R²⁰.
 9. The compound of claim 8, wherein Q is a heterocycle selected from the group consisting of azetidinyl, pyrrolidinyl, piperidyl, piperazinyl, 4-acetylpiperazinyl, 4-methylsulfonylpiperazinyl, morpholinyl, thiomorpholinyl, 1,1-dioxo-1λ⁶-thiomorpholinyl, 2,6-dimethylmorpholinyl, and 3,5-dimethylmorpholinyl, wherein Q is connected to —C(═O)— via a ring nitrogen atom; R¹ is independently a member selected from the group consisting of cyclohexylmethyl, 4,4-dimethyl-cyclohexylmethyl, spiro[2.5]oct-6-ylmethyl, spiro[3.5]non-7-ylmethyl, cyclohexylethyl, cyclopentylmethyl, cyclopentylethyl, cyclobutylmethyl, cyclobutylethyl, cyclopropylmethyl, cyclopropylethyl, allyl, butenyl, cyclopentyl, cyclohexyl, t-butylmethyl, t-butylethyl, 4-ethyl-4-hydroxyl-cyclohexylmethyl, phenethyl, phenylpropyl, phenyl, 4-fluorophenyl, 4-methylphenyl, 4-trifluoromethylphenyl, 4-chlorophenyl, 4-methoxyphenyl, 4-trifluoromethoxyphenyl and 4-methylsulfonylphenyl; R⁴ is H or methyl; R⁵ is a member selected from the group consisting of H, methyl, ethyl, propyl, i-propyl, butyl, i-butyl, cyclopropyl, cyclopropylmethyl, methylsulfonylmethyl, methylsulfonylethyl, hydroxylmethyl, hydroxyethyl, phenethyl, benzyl, phenyl and benzyloxymethyl; Ar is phenyl substituted with 0-3 R²⁰, isoxazolyl substituted with 0-2 R²⁰, pyridyl substituted with 0-2 R²⁰; each R²⁰ is independently a member selected from the group consisting of H, F, Cl, OCH₃, CH₃, SCH₃, S(═O)₂CH₃, S(═O)₂NH₂, acetyl, C(═O)NH₂, CO₂H, OCF₃ and OCHF₂; alternatively, R²⁰ and R⁹ are taken together to form a 5-membered heterocyclic ring containing 1 nitrogen atom; wherein said 5-membered heterocyclic ring is ortho-fused to Ar; wherein said 5-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴; each R²⁴ is independently a member selected from the group consisting of methyl, and F; alternatively, two R²⁴ may be combined to form cyclopropyl and cyclobutyl.
 10. The compound of claim 3, wherein R⁵ is a member selected from the group consisting of H, phenyl substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)₂—; each R²¹ is independently a member selected from the group consisting of H, OH, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, C₁-C₄ alkoxy, phenyl substituted with 0-3 R¹³, and C₃-C₈ cycloalkyl.
 11. The compound of claim 10, wherein Q is a heterocycle selected from the group consisting of azetidinyl, pyrrolidinyl, piperidyl, piperazinyl, 4-acetylpiperazinyl, 4-methylsulfonylpiperazinyl, morpholinyl, thiomorpholinyl, 1,1-dioxo-1λ⁶-thiomorpholinyl, 2,6-dimethylmorpholinyl, and 3,5-dimethylmorpholinyl, wherein Q is connected to —C(═O)— via a ring nitrogen atom; R¹ is independently a member selected from the group consisting of cyclohexylmethyl, 4,4-dimethyl-cyclohexylmethyl, spiro[2.5]oct-6-ylmethyl, spiro[3.5]non-7-ylmethyl, cyclohexylethyl, cyclopentylmethyl, cyclopentylethyl, cyclobutylmethyl, cyclobutylethyl, cyclopropylmethyl, cyclopropylethyl, t-butylmethyl, t-butylethyl, phenylmethylsulfonylmethyl and benzylsulfanylmethyl; R⁵ is a member selected from the group consisting of H, methyl, ethyl, propyl, i-propyl, butyl, i-butyl, cyclopropyl, cyclopropylmethyl, methylsulfonylmethyl, methylsulfonylethyl, hydroxylmethyl, hydroxyethyl, benzyloxymethyl, phenethyl, benzyl and phenyl; Ar is phenyl substituted with 0-3 R²⁰, pyridinyl substituted with 0-2 R²⁰, and isoxazolyl substituted with 0-2 R²⁰; each R²⁰ is independently a member selected from the group consisting of H, F, Cl, CH₃, OCH₃, SCH₃, S(═O)₂CH₃, S(═O)₂NH₂, acetyl, C(═O)NH₂, CO₂H, OCF₃ and OCHF₂; alternatively, R²⁰ and R⁹ are taken together to form a 5-membered heterocyclic ring containing 1 nitrogen atom; wherein said 5-membered heterocyclic ring is ortho-fused to Ar; wherein said 5-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴; each R²⁴ is independently a member selected from the group consisting of methyl, ethyl and F; alternatively, two R²⁴ may be combined to form cyclopropyl and cyclobutyl.
 12. The compound of claim 4, wherein R⁵ is a member selected from the group consisting of H, phenyl substituted with 0-2 R¹³, C₃-C₇ cycloalkyl, C₁-C₆ alkyl substituted with 0-1 R²¹, wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—, —S—, —S(═O)₂—; each R²¹ is independently a member selected from the group consisting of H, OH, C(═O)OR¹⁴, C(═O)NR¹⁵R¹⁶, NR²²R²³, C₁-C₄ alkoxy, phenyl substituted with 0-3 R¹³, and C₃-C₈ cycloalkyl; R⁷ is H.
 13. The compound of claim 12, wherein Q is a heterocycle selected from the group consisting of azetidinyl, pyrrolidinyl, piperidyl, piperazinyl, 4-acetylpiperazinyl, 4-methylsulfonylpiperazinyl, morpholinyl, thiomorpholinyl, 1,1-dioxo-1λ⁶-thiomorpholinyl, 2,6-dimethylmorpholinyl, and 3,5-dimethylmorpholinyl, wherein Q is connected to —C(═O)— via a ring nitrogen atom; and NR²⁵R²⁶; R³ is independently a member selected from the group consisting of cyclohexylmethyl, 4,4-dimethyl-cyclohexylmethyl, spiro[2.5]oct-6-ylmethyl, spiro[3.5]non-7-ylmethyl, cyclohexylethyl, cyclopentylmethyl, cyclopentylethyl, cyclobutylmethyl, cyclobutylethyl, cyclopropylmethyl, cyclopropylethyl, t-butylmethyl, t-butylethyl, phenyl, cyclohexyl, cyclopentyl, 4,4-dimethylcyclohexyl, phenylmethylsulfonylmethyl and benzylsulfanylmethyl; R⁵ is a member selected from the group consisting of H, methyl, ethyl, propyl, i-propyl, butyl, i-butyl, cyclopropyl, cyclopropylmethyl, methylsulfonylmethyl, methylsulfonylethyl, hydroxylmethyl, hydroxyethyl, phenethyl, benzyl, phenyl and benzyloxymethyl; Ar is phenyl substituted with 0-3 R²⁰, and isoxazolyl substituted with 0-2 R²⁰; each R²⁰ is independently a member selected from the group consisting of H, F, Cl, OCH₃, CH₃, SCH₃, S(═O)₂CH₃, S(═O)₂NH₂, —S(═O)₂NH(C═O)CH₃, acetyl, C(═O)NH₂, CO₂H, OCF₃ and OCHF₂; alternatively, R²⁰ and R⁹ are taken together to form a 5-membered heterocyclic ring containing 1 nitrogen atom; wherein said 5-membered heterocyclic ring is ortho-fused to Ar; wherein said 5-membered heterocyclic ring may be optionally substituted with 0-2 R²⁴; each R²⁴ is independently a member selected from the group consisting of methyl, and F; alternatively, two R²⁴ may be combined to form cyclopropyl and cyclobutyl. each of R²⁵ and R²⁶ is independently a member selected from the group consisting of C₁-C₄ alkyl; wherein said C₁-C₆ alkyl may optionally contain a heteroatom selected from the group consisting of —O—.
 14. The compound of claim 1, wherein said compound is selected from the group consisting of: Morpholine-4-carboxylic acid (S)-2-cyclohexyl-1-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl ester; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[2-(4-fluoro-phenylamino)-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[2-(4-methoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide; [2-(4-Fluoro-phenylamino)-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; 2-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; [4-(R)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-tetrahydro-furan-3-(R)-yl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; 2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 3-(R)-Cyclohexylmethyl-N-[2-(4-fluoro-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; [1-(R)-Benzyloxymethyl-2-(5-fluoro-2,3-dihydro-indol-1-yl)-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; Morpholine-4-carboxylic acid 2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylcarbamoyl]-ethyl ester; Morpholine-4-carboxylic acid 2-cyclohexyl-1-(S)-[2-(5-fluoro-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethylcarbamoyl]-ethyl ester; 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; 2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclopentylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclopentylmethyl-3-(R)-methyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; Morpholine-4-carboxylic acid {2-benzylsulfanyl-1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {1-(R)-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethylcarbamoyl]-2-phenylmethanesulfonyl-ethyl}-amide; (R)-2-Cyclohexylmethyl-N-[2-(4-methoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; 2-(R)-(2-Cyclohexyl-ethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 2-(R)-Cyclohexylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 5,5-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic acid [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide; 4,4-Dimethyl-2-(R)-(2-morpholin-4-yl-2-oxo-ethyl)-pentanoic acid [1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-amide; 2-(R)-Cyclopentylmethyl-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; 2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclohexylmethyl-N-{3-methanesulfonyl-1-(R)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-5-phenyl-pentanoic acid {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide; 2-(R)-Cyclopentylmethyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-phenethyl-butyramide; 2-(R)-(2-Cyclopentyl-ethyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide; 2-(S)-Cyclohexyl-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 4-Morpholin-4-yl-4-oxo-2-(R)-phenethyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-butyl}-butyramide; 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-{3-phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-butyramide; 2-(S)-(4-Fluoro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(S)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-(4-Chloro-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 4-Morpholin-4-yl-4-oxo-2-(S)-phenyl-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; 2-(R)-Cyclohexylmethyl-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; 2-(R)-(2-Cyclopentyl-ethyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; 2-(R)-(2-Morpholin-4-yl-2-oxo-ethyl)-pent-4-enoic acid {2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-amide; 2-(S)-(4-Chloro-phenyl)-4-morpholin-4-yl-4-oxo-N-[1-(S)-phenyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-butyramide; (R)-5,5-Dimethyl-2-(2-morpholin-4-yl-2-oxo-ethyl)-hexanoic acid [2-(5-methyl-isoxazol-3-ylamino)-ethyl]-amide; 2-(R)-Cyclohexylmethyl-4-(cis-2,6-dimethyl-morpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; 2-(R)-Cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-4-thiomorpholin-4-yl-butyramide; 4-(4-Acetyl-piperazin-1-yl)-2-(R)-cyclohexylmethyl-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; 2-(S)-(4-Methoxy-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; 2-(R)-Cyclohexylmethyl-N-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; -{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(R)-(4-trifluoromethyl-phenyl)-butyramide; N-{2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-2-(S)-p-tolyl-butyramide; 2-(R)-Cyclohexylmethyl-4-(1,1-dioxo-thiomorpholin-4-yl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-oxo-butyramide; 2-(R)-(3-Ethyl-3-hydroxy-cyclohexylmethyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-phenyl-butyramide; 2-(S)-(4-Fluoro-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(S)-(4-Methoxy-phenyl)-N-[1-(S)-methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-butyramide; 2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(6-methoxy-pyridin-3-ylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide; N-[1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(R)-spiro[2.5]oct-6-ylmethyl-butyramide; N-[2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; 2-(S)-(4-Fluoro-phenyl)-N-{1-(S)-[(3-methanesulfonyl-phenylamino)-methyl]-2-methyl-propyl}-4-morpholin-4-yl-4-oxo-butyramide; N-[1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; N-[1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; N-[1-(S)-Cyclopropyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-4-morpholin-4-yl-4-oxo-2-(S)-(4-trifluoromethyl-phenyl)-butyramide; 2-(R)-(4-methanesulfonyl-phenyl)-N-{2-methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-4-morpholin-4-yl-4-oxo-butyramide; (S,S)-Morpholine-4-carboxylic acid {2-cyclohexyl-1-[2-(4-methoxy-phenylamino)-1-methyl-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclopentyl-1-(S)-[2-(4-methoxy-phenylamino)-1-(S)-methyl-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid (2-cyclohexyl-1-(S)-{3-methanesulfonyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide; Morpholine-4-carboxylic acid (2-cyclohexyl-1(S)-{1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propylcarbamoyl}-ethyl)-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[2-methyl-1-(S)-(pyridin-3-ylaminomethyl)-propylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[1-(S)-methyl-2-(5-methyl-isoxazol-3-ylamino)-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {1-(S)-[2-(benzothiazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide; Morpholine-4-carboxylic acid {1-(S)-[2-(benzooxazol-2-ylamino)-1-(S)-methyl-ethylcarbamoyl]-2-cyclohexyl-ethyl}-amide; (S,S)-Morpholine-4-carboxylic acid {2-cyclohexyl-1-[2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; Morpholine-4-carboxylic acid {2-cyclohexyl-1-(S)-[1-(S)-cyclopropyl-2-(5-fluoro-3,3-spirocyclopropyl-2,3-dihydro-indol-1-yl)-ethylcarbamoyl]-ethyl}-amide; [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [1-(S)-Methyl-2-(4-trifluoromethoxy-phenylamino)-ethyl]-carbamic acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl ester; [2-(4-Difluoromethoxy-phenylamino)-1-(S)-methyl-ethyl]-carbamic acid 3,3-dimethyl-1-(S)-(morpholine-4-carbonyl)-butyl ester; {2-Methyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; {3-Phenyl-1-(S)-[(4-trifluoromethoxy-phenylamino)-methyl]-propyl}-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; {1-(S)-[(4-Trifluoromethoxy-phenylamino)-methyl]-butyl}-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [2-(4-Acetylsulfamoyl-phenylamino)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [1-(S)-Methyl-2-(5-methyl-isoxazol-3-ylamino)-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; (S,S)-[1-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl-methyl)-propyl]-carbamic acid 1-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl ester; (S,S)-[2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-methyl-ethyl]-carbamic acid 2-(4-acetyl-piperazin-1-yl)-1-cyclohexylmethyl-2-oxo-ethyl ester; [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl ester; [2-(5-Fluoro-2,2-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-(2,6-cis-dimethyl-morpholin-4-yl)-2-oxo-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 2-(4-acetyl-piperazin-1-yl)-1-(S)-cyclohexylmethyl-2-oxo-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-(4-methanesulfonyl-piperazin-1-yl)-2-oxo-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-oxo-2-thiomorpholin-4-yl-ethyl ester; [2-(5-Fluoro-3,3-spiro-cylopropyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-oxo-2-piperidin-1-yl-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-oxo-2-pyrrolidin-1-yl-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 2-cyclohexyl-1-(S)-dimethylcarbamoyl-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-(1,1-dioxo-1λ⁶-thiomorpholin-4-yl)-2-oxo-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 2-cyclohexyl-1-(S)-[(2-methoxy-ethyl)-methyl-carbamoyl]-ethyl ester; [2-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-1-(S)-methyl-ethyl]-carbamic acid 2-azetidin-1-yl-1-(S)-cyclohexylmethyl-2-oxo-ethyl ester; [1-(S)-Cyclopropyl-2-(5-fluoro-3,3-dimethyl-2,3-dihydro-indol-1-yl)-ethyl]-carbamic acid 1-(S)-cyclohexylmethyl-2-morpholin-4-yl-2-oxo-ethyl ester; [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic acid 2-morpholin-4-yl-2-oxo-1-(R,S)-phenyl-ethyl ester; [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic acid 2-morpholin-4-yl-2-oxo-1-(R)-phenylmethanesulfonylmethyl-ethyl ester; and [1-(S)-(5-Fluoro-3,3-dimethyl-2,3-dihydro-indol-1-ylmethyl)-2-methyl-propyl]-carbamic acid 1-(S)-cyclohexyl-2-morpholin-4-yl-2-oxo-ethyl ester.
 15. A pharmaceutical composition, said composition comprising a compound of claim 1 and an excepient.
 16. A method of inhibiting cathepsin S activity in a mammal in need thereof, said method comprising: administering to said mammal a therapeutically effective amount of a compound of claim
 1. 17. The method of claim 16, wherein the cathepsin S inhibition constant for said compound of Formula I is less than 10 μM.
 18. The method of claim 17, wherein the cathepsin S inhibition constant for said compound of Formula I is less than 1.0 μM.
 19. The method of claim 18, wherein the cathepsin S inhibition constant for said compound of Formula I is less than 0.1 μM.
 20. The method of claim 16, wherein cathepsin S is selectively inhibited in the presence of at least one other cathepsin.
 21. The method of claim 20, wherein the inhibition constant of said compound of Formula I for said at least one other cathepsin is at least 10 times greater than the cathepsin S inhibition constant of said compound of Formula I.
 22. The method of claim 21, wherein the inhibition constant of said compound of Formula I for said at least one other cathepsin is at least 100 times greater than the cathepsin S inhibition constant of said compound of Formula I.
 23. The method of claim 22, wherein the inhibition constant of said compound of Formula I for said at least one other cathepsin is at least 1000 times greater than said cathepsin S inhibition constant of said compound of Formula I.
 24. The method of claim 16, wherein said compound is selected from the compounds of claim
 14. 